RFC1 Antibodies

Replication factor C subunit 1 (RFC1)

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.

Binds the PO-B transcription component in addition to other GA rich DNA sequences. Could play a role in DNA transcription regulation in addition to DNA replication and/or repair.

Gene Information

Human
Gene Name:	RFC1
Uniprot:	P35251
Entrez:	5981

Family

Belongs to:
activator 1 large subunit family

Alternative Names

A1 140 kDa subunit; A1; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; MGC51786; MHC binding factor, beta; MHCBFB; PO-GA; RECC1; replication factor C (activator 1) 1 (145kD); replication factor C (activator 1) 1, 145kDa; Replication factor C 140 kDa subunit; Replication factor C large subunit; replication factor C subunit 1; replication factor C1; RF-C 140 kDa subunit; RFC; RFC1; RFC140; RFC140DNA-binding protein PO-GA

Molecular Weight

Mass (kDA):
128.255 kDA

Genomic Context

Human
Location:	4p14
Sequence:	4; NC_000004.12 (39287456..39366381, complement)

Tissue Specificity

Wide tissue distribution. Undetectable in placental tissue.

Subcellular Localizations

Nucleus.

Diseases

• 5,10-methylenetetrahydrofolate Reductase Deficiency
• Malignant Neoplasms
• Leukemia
• Acute Lymphocytic Leukemia
• Neoplasms
• Malignant Neoplasm Of Breast
• L1 Acute Lymphoblastic Leukemia
• Leukemia L1210
• Folic Acid Deficiency
• Congenital Abnormality
• Mammary Neoplasms
• Carcinoma
• Cleft Palate

• Cleft Lip
• Down Syndrome
• Hyperhomocysteinemia
• Cardiovascular Diseases
• Lymphoma
• Dysequilibrium Syndrome
• Lung Neoplasms

Interacting Proteins

• RFC2

Pathways

• Transport
• Dna Replication
• Dna Repair
• Cell Cycle
• Methylation
• Dna Damage Checkpoint
• Dna Methylation
• S Phase
• Localization
• Drug Resistance
• Cell Proliferation
• Mismatch Repair
• Sister Chromatid Cohesion

• Methotrexate Transport
• Cell Cycle Arrest
• Cell Growth
• Cell Division
• Drug Transport
• Translesion Synthesis
• Mitotic Recombination

PTMs

• Methylation
• Phosphorylation
• Reduction
• Ubiquitination

• Demethylation
• Cleavage
• Deacetylation
• Acetylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RFC1

Best Uses of the RFC1 Marker From Boster Bio

This article will talk about the best uses and benefits of the Anti-Replication factor Csubunit 1 marker. This marker is important in many research fields, including immunofluorescence. There are many flow procedures to choose from. This article will provide guidance on how to optimize your experiment using this marker. You can also find information about Boster Bio from Immunofluorescence and ICC.

Boster Bio

There are many options to choose from when it comes time to determine the best flow process for an experiment. Boster Bio's RFC1 indicator is one of them. This antibody reacts with Rat, Mouse, and Human. Scientists can use this antibody to analyze species-specific gene expression or applications, and receive product credits. This antibody is available worldwide and can be used in a variety research applications. Learn more about RFC1's uses and benefits here.

Anti-Replication factor C subunit 1

The Anti Replication factor C subunit 1 marker (ARF-11) can be used for many purposes. This antibody was tested against Human DNA using IHC and WB. It is also available in blocking-peptide form. Boster Bio's Catalog Number PA1716 has more information. Boster Bio is the authorized seller of this antibody.

ICC

Based on a recent study that demonstrated that the RFC1 marker distinguishes bladder cancer from non-cancer cells, the ICC Best Usages of the RFC1 markers are based. This supports the notion that the RFC1 or ENT1 markers are associated with malignancy and progression in certain kinds of cancer. These findings are consistent in the same way as those obtained from immunostained-tissue specimens.

75 cytological sample were used to test for RFC1/ENT1 expression. 57 of these were evaluated using immunocytochemistry. The remaining samples did not have cancer cells or had poor stainability. The majority of cytological specimens showed high expression of the ENT1 marker and RFC1. Additionally, high expression levels of the RFC1-ENT1 markers were associated with a high histological score and a higher MIB-1 index.

The expression levels of the transporters were nearly identical in bladder cancers that were both non-invasive and invasive. Multiple logistic regression analysis found that RFC1 (and ENT1) markers could accurately predict progression of invasive bladder tumors. RFC1 markers and ENT1 markers were associated with aggressive tumor development. If you're looking for more information on ICC Best Uses of The RFC1 Marker, then look no further.

Immunofluorescence

Boster Bio has validated RFC1 antibody on a wide variety of platforms including cell lines, tissues, tissues, and tissues. They are available in different concentrations and each antibody has specific properties that enable it to perform specific tasks. Boster Bio’s antibody to SLC19A1 works with Rats, Monkeys, Rats, Mouses, Rats, and Rats. Flow procedures vary depending on the sample type, but the results are typically the same.

The antibody can be used in either direct staining or indirect staining. Direct immunofluorescence stains use antibodies directly conjugated fluorescence. The benefits of this method include eliminating non-specific binding, which is a major issue when using secondary antibodies. Secondary antibodies can fail to enter cells because of non-specific binding. This makes indirect staining a great choice for targeting specific proteins.

Optimization

The RFC1 marker is an antibody produced by the Boster Bio company. This marker allows scientists to test the specificity and compatibility of applications and species. Researchers can submit their results to receive product credits. Boster bio has a variety of products and flow procedures for scientists. The following articles will help you learn more about the RFC1 marker and how it can benefit your experiments. These articles are meant for scientists all over the world.