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- Table of Contents
Facts about Ras GTPase-activating protein 2.
Inhibitory regulator of This Ras-cyclic AMP pathway.
Binds inositol tetrakisphosphate (IP4)..
Human | |
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Gene Name: | RASA2 |
Uniprot: | Q15283 |
Entrez: | 5922 |
Belongs to: |
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No superfamily |
RASA2 RAS p21 protein activator 2; RASA2
Mass (kDA):
96.614 kDA
Human | |
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Location: | 3q23 |
Sequence: | 3; NC_000003.12 (141487027..141615356) |
Cytoplasm. Cytoplasm, perinuclear region.
The Boster Bio Anti–RASA2 marker is available for purchase. Read on for more information about this Biological reagent, how to use it, and customer reviews. You'll also find out about its biological uses. This will help you understand how this reagent works, and how it can benefit your research. Here are some of our favorite uses of RASA2 markers.
Boster Bio Anti-RASA2 Markers can be used in research studies on this protein. These antibodies react with human, mouse and rat tissues. Boster antibodies are highly specific and are frequently cited in scientific literature. They are validated using Immunohistochemistry, Western Blotting, and ELISA. They are used in numerous research studies to determine levels of protein.
Two potential substrate proteins for RASA2's palmitoylation have been identified through molecular biology research. The RASA2 protein's localization can be affected by palmitoylation or proteolysis. Palmitoylation gives Ras2 a greater hydrophobicity, which drives it towards the plasma membrane. Rce1A, and CppB are potential homologs for the RCE1 proteins. They were both named after fungi. The CppB is most likely a homolog for Ste24p.
These mutations affect RasA activity and localization. These deletions lead to changes in RasA activation, morphogenesis, and germination. The deletions cppB/A can also affect the expression and localization RASA2 in cells. These effects indicate that RasA can affect the localization and activation RASA2.
RASA2 mutation C207 causes an increase in the accumulation of the protein in cell-periphery. This is further exacerbated by mutant RasA's loss of CAAX proteolysis. The molecular biology studies of the RASA2 protein marker are now underway. We need to first understand how RASA2 proteins are made in cells before we can start looking at those applications. How can we assess the quality of RASA2 protein?
The RASA2 marker in mammalian cells is a versatile molecular tool. Proper localization is essential for its function in fungal growth and virulence. Proper localization of Ras2p depends on a combination of post-translational modifications that target the RASA2 protein's C-terminal CAAX box. Due to the presence uncleaved-AAX amino acids, DcppB/DcppA is less apt to attach to the membrane.
A 10X solution of 40% sucrose with 0.17% bromophenol Blue and xylene Cyanol is a biological reagent for the RASE2 marker. As an ultra pure molecular biologist grade powder, a 25X buffer for TAE (40 mM Tris Acetate, 1 mM N2EDTA) is another reagent.
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PMID: 8917095 by Kobayashi M., et al. Human rasGTPase-activating protein (human counterpart of GAP1m): sequence of the cDNA, primary structure of the protein, production and chromosomal localization.
PMID: 8812506 by Li S., et al. cDNA cloning and chromosomal mapping of a novel human GAP (GAP1M), a GTPase-activating protein of Ras.