RAD51AP1 Antibodies

RAD51-associated protein 1 (RAD51AP1)

May participate in a common DNA damage response pathway linked to the activation of homologous recombination and double-strand fracture repair. Functionally cooperates with PALB2 in boosting of D-loop creation by RAD51.

Binds to single and double stranded DNA, and is capable of aggregating DNA. Also binds RNA.

Gene Information

Human
Gene Name:	RAD51AP1
Uniprot:	Q96B01
Entrez:	10635

Family

Belongs to:
No superfamily

Alternative Names

PIR51RAD51-interacting protein; RAD51 associated protein 1; RAD51-associated protein 1

Molecular Weight

Mass (kDA):
38.457 kDA

Genomic Context

Human
Location:	12p13.32
Sequence:	12; NC_000012.12 (4538890..4560047)

Subcellular Localizations

Nucleus. Colocalizes with RAD51 to multiple nuclear foci.

Diseases

• Neoplasms
• Malignant Neoplasms
• Carcinoma
• Mammary Neoplasms
• Fanconi Anemia
• Malignant Neoplasm Of Breast
• Lymphoma
• Cell Transformation, Neoplastic
• Anemia
• Cholangiocarcinoma
• Oropharyngeal Disorders
• Fracture Of First Cervical Vertebra
• Bile Duct Neoplasms

• Radiation Injuries, Experimental
• Chromosome Break
• Neoplasms, Experimental
• Head And Neck Carcinoma
• Inflammatory Response
• Fibrosis
• Oropharyngeal Neoplasms

Interacting Proteins

• DMC1
• PALB2
• RAD51
• SIAH1

Pathways

• Dna Repair
• Cell Cycle
• Cell Proliferation
• Meiosis
• Recombinational Repair
• Male Meiosis
• Proteolysis
• Metaphase
• Chromosome Segregation
• Inflammatory Response
• Dna Replication
• Reverse Transcription
• Response To Ionizing Radiation

• Translation
• Meiotic Chromosome Segregation
• Prophase
• Cell Differentiation
• Histone Acetylation
• Zygotene
• Rna Processing

PTMs

• Phosphorylation

• Acetylation

• Methylation

Research Areas

• DNA Repair
• Homologous Recombination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RAD51AP1

Boster Bio: Best Uses Of The RAD51AP1 Marker

The RAD51AP1 marker is a highly versatile and potent biological marker. It can be used in conjunction with other treatments to detect DNA damage or enhance the function DNA repair pathways. Boster scientists are able to submit their results for different species/applications and receive product credits. Its use is not limited to scientists in the United States, but is applicable to scientists around the world.

RAD51AP1 Signer

The RAD51AP1 gene is a transcription factor involved in homologous recombination in cells, a process essential for maintaining genomic stability and suppressing tumor development. It plays a crucial role in maintaining mitochondrial copy number. This gene, also known RAD51 is found in high levels in the tissues from cancer patients, including liver carcinoma. It is also involved in the regulation of apoptosis within cancer cells.

Tachon et.al. carried out a large-scale analysis on RAD51 proteins. Tachon et.al. found that RAD51 protein expression was significantly associated with Ku70, which is associated to poor chemotherapy response. These results suggest that RAD51 is an emerging therapeutic target. They also discovered that RAD51AP1 gene expression was associated RAD51AP1 expression with disease-free survivability in patients with nonsmall-cell lung carcinoma (NSCLC). They also discovered that RAD51AP1 could be knocked down to decrease radioresistance in NSCLC cell lines.

The expression of RAD54B in liver cancer patients may be associated with their prognosis. However, there are still many unanswered questions regarding how RAD54B may relate to liver cancer. There are no reports that the RAD54B gene has been found in liver cancer cells. This gene might be present in the tumors of patients with liver cancer.

Activation of DNA damage and repair pathways

The RAD51AP1 protein encoded by the gene encodes a protein critical for the HR pathway. This protein is enriched by chromatin fractions, and binds DNA with reduced affinity in cancer cells. Depletion or loss of RAD51AP1 results a decrease in recruitment of HR-proteins to chromatin. Activating DNA damage repair pathways using the RAD51AP1 marker may help identify new cancer treatment strategies that target the RAD51AP1 genes.

This gene has several important applications for activating DNA damage repair mechanisms. Activation RAD51 recombinase has been a popular therapeutic target for cancer therapy. However, it also has several compensatory pathways that could be targeted by HR inhibiters. It is therefore crucial to understand the functions of RAD51AP1 in order to design effective therapies against this disease.

Understanding DNA repair pathways can help in the development of new inhibitors. Certain DNA damaging substances can have an anti-cancer impact by activating DNA repair pathways. Clinical trials are currently underway for inhibitors to target these pathways. However, there are some potential drawbacks to this treatment. RAD51AP1 inhibitors may have adverse effects in normal tissues.

Fanconi anemia may be represented by activation DNA damage repair pathway with RAD51AP1. This gene is located on chromosome 12p13.1-13.2 and shares high sequence similarity with the mouse Rad51ap1 genes. It is highly conserved in vertebrates. Although RAD51AP1 has not been found in model organisms, it can be found in many invertebrates.

The behavior of tumor cells may also be affected by activating DNA damage repair pathways in cancer cell lines through RAD51AP1 gene knockdown. This pathway has been linked to cancer metastasis, and is enriched by tumor cells. Furthermore, tumor cells that overexpress the RAD51AP1 gene have a high risk of metastasis. You may be looking for a new method to monitor your cancer cell growth. The activation of DNA repair pathways with RAD51AP1 could be the answer.

Combination therapy

A new gene called RAD51AP1 has been used to create a novel treatment for metastatic breast cancer. This gene is part of RAD51, which also has a few other functions. Combination therapy can be used with the RAD51AP1 marker. This therapy can be used to target specific areas of the tumor that are sensitive to a particular agent.

In this study, OC cells were transfected with siRNAs containing the RAD51AP1 target gene. The cells were cultured in RPMI-1640 medium without FBS for 24 hours, and transfected with siRNAs containing either the RAD51AP1 target gene or the control gene. TGFb was used to reverse the siRNA-inhibited effects of siRAD51AP1.

The tumor cell-specific expression of the RAD51AP1 genes is high. It is epistatic to XRCC3 which is essential for DNA damage repair in the S phase cells. However, the exact mechanism of its action is still unknown. To determine whether this gene is useful for developing cancer treatments, researchers will continue to examine it. While there are no known clinical trials yet to test the efficacy of the treatment, it could become a valuable tool for cancer researchers.

The study examined the relationship between gene mutations, tumour microenvironment, and other factors. It also established a RAD51AP1-related nomogram. The findings also suggested that patients with higher levels of the RAD51AP1 genes had lower survival rates. These findings suggest that OC progression could be facilitated by upregulating this gene. This gene could also play a role in the development of the disease.

Primary antibodies of high-affinity

High-affinity primary antibodies are required for detection of RAD51AP1 via immunohistochemistry. Molecular weight standards are preferably less than 200 kDa. Vectashield and mCherry GFP-LC3II expression plasmids are suitable reagents. These products are supplied with 2 Ug/mL DAPI. The RAD51AP1 marker may be detected in a variety different ways.

Your immunohistochemistry studies can be reduced by using a high-affinity antibody to detect RAD51AP1. It is important to use this marker in immunohistochemistry, as RAD51AP1 can be found in the nucleus cancer cells. This marker could be involved in cell proliferation and DNA damage regulation. However, it isn't known if it plays a role as a cancer marker.

24 h post-irradiation, cells expressing RAD51AP1 were able to detect the protein. The protein colocalized in cells with gH2AX. Positive is defined as the presence of five or more foci. Randomly selected cells at each timepoint were used to evaluate the expressions of RAD51AP1 gH2AX. High-affinity primary antibodies are the best uses of the RAD51AP1 marker

The RAD51AP1 marker is a highly effective biomarker. It is an effective marker for studying meiotic processes. Various studies have shown that RAD51 is essential for meiotic repair. Shinohara et al. studied RAD51 in 1992 and Rinaldo et al. It was studied in 2002. Later, RAD51 was identified. It is also associated to a signaling protein called DMC1 or gH2Ax.

The analysis of autophagic fluid is another application for the RAD51AP1 markers. In this study, researchers found that RAD51AP1 KO cell lines exhibited increased accumulation of light chain 3 protein A, which is essential for autophagosome biogenesis. Moreover, RAD51AP1 KO ALT+ cell lines showed a moderate rise in LAMP1, which is indicative of the autophagic flux. Interestingly, bafilomycin A1 reversed this reporter system.

Manufacturers

A new glioma-gene, RAD51AP1, was identified as an oncogene. It activates the formation a protein known as joint molecule. This is required for cellular protection against DSB inducing agents. It has been previously identified in lung cancer and melanoma, but not in glioma. This study could be the first one to identify RAD51AP1 an oncogene for glioma.

Several methods were used to identify RAD51AP1 in the genome. One method involved mixing purified GST-NUCKS1 with 32P-labeled DNA substrats. Protein-DNA complexes were resolved by 8% native polyacrylamide gel electrophoresis in TAE buffer (40 mM Tris pH, 20 mM acetic acid).

The purified RAD51AP1 marker is compatible with the RAD51 gene. It interacts avidly with RAD51. This marker was not compatible before with RAD51. Its purification process is described in (6). This protein can be used as a paralog for RAD51. Its C terminal domain facilitates interaction to RAD51. Mutational analysis has identified critical residues in the domain. Below are the boxes that list the residues.