RAB6A Antibodies

Ras-related protein Rab-6A (RAB6A)

Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER).

Has a very low GTPase activity. Involved in COPI-independent retrograde transport from the Golgi to the ER (PubMed:25962623).

Gene Information

Human
Gene Name:	RAB6A
Uniprot:	P20340
Entrez:	5870

Family

Belongs to:
small GTPase superfamily

Alternative Names

GTP-binding protein RAB6A'; GTP-binding protein RAB6B; Oncogene RAB6; Rab GTPase; Rab-6; RAB6, member RAS oncogene family; Rab6A variant 3; RAB6A'; RAB6A, member RAS oncogene family; RAB6B; RAB6rab-6; ras-related protein Rab-6A

Molecular Weight

Mass (kDA):
23.593 kDA

Genomic Context

Human
Location:	11q13.4
Sequence:	11; NC_000011.10 (73675638..73761074, complement)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Golgi apparatus membrane; Lipid-anchor. BICD2 facilitates its targeting to Golgi apparatus membrane.; [Isoform 1]: Golgi apparatus membrane; Lipid-anchor.; [Isoform 2]: Golgi apparatus membrane; Lipid-anchor.

Diseases

• Neuroblastoma
• Hypopigmentation-immunodeficiency Disease
• Lymphohistiocytosis, Hemophagocytic
• Legionnaires' Disease
• Prion Diseases
• Adenocarcinoma
• Cell Transformation, Neoplastic
• Melanoma
• Neurodegenerative Disorders
• Endometrial Adenocarcinoma
• Endometrial Neoplasms
• Oculocerebrorenal Syndrome

• Endometrial Polyp
• Cholera
• Stomatitis
• Vesicular Stomatitis
• Malignant Neoplasms
• Nervousness
• Endometrial Carcinoma

Interacting Proteins

• Dennd5a
• GCC2
• OCRL
• PMM1

Pathways

• Transport
• Intracellular Transport
• Mitosis
• Metaphase
• Endocytosis
• Cell Cycle
• Cell Differentiation
• Cell Growth
• Secretion
• Sulfation
• Secretory Pathway
• Response To Stress
• Glycosylation

• Ion Transport
• Protein Transport
• Localization
• Anaphase
• Melanosome Transport
• Regulation Of Intracellular Transport
• Exocytosis

PTMs

• Glycosylation

• Geranylgeranylation

• Ubiquitination

Research Areas

• Signal Transduction

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RAB6A

Best Uses Of The RAB6A Marker

The RAB6A protein marker has many uses in biomedical science. It can be used in Autoradiography, QuantitativePCR and staining astrocytes. These markers can be used by scientists all over the world and they will receive product credits. In this article, we will explore some of the most common uses of this biomarker. Let's get started.

Autoradiography

The RAB6A antibody marker reacts with human, mouse and rat. This antigen is selective for astrocytes, and is not present in neurons, microglia, oligodendrocytes, NG2 cells, or other glial cell types. Researchers can submit data for species and applications studies, and receive product credit.

The GS and Aldh2L1 markers stain astrocytes better than GFAP or Aldh2L1 cells (GFAP and Rab6A, respectively). They stain 100% GFAP+/Rab6A+ cell lines, and GS/Aldh2L1 stain astrocytes across all brain regions. These data support the use of Rab6A in a pan-astrocytic manner.

Quantitative PCR

RTPCR amplified a portion of the Rab6A' cDNA from human endothelial and used the resulting sequence as a probe for a cDNA collection. This study resulted in 8 x104 independent Clones. Positive clones were sequenced using a Sanger method and a T7 sequencing kit. The sequence data to human Rab6A have been submitted at GenBank and DDBJ databases.

Rab6A is an essential component in chromatin. A single copy of the DNA encoding it could represent thousands of copies a particular gene. The RAB6A marker can be used to analyze gene expression in human cells. It can detect a wide range in genetic mutations in human cells. Rab6A is a conserved, human-specific tRNA. It has a high level of identity among human cells. Its isoforms exhibit functional differences between mouse and human cells.

The Rab6A gene can be found on two chromosomes. The Rab6A gene contains two exons, the first being a duplicate. This indicates that Rab6A is encoded in distinct genes. Rab6A' isoforms are generated through alternative use of a homologous exon. Multiple chromosomes have been linked to Rab6A.

It has been proven that Rab6A's protein is a significant regulator in the activity transmembrane secretory enzymes and luminal secretory ones. Rab6A'sQ72L reduces SEAP secretion in half. Rab6A’s siRNA is a significant inhibitor of EE/RE/TGN transport. Rab6A's knockdown results in cell secretion inhibition and important implications for cancer research.

Staining astrocytes

This staining was performed in neonatal mice to identify the subtypes. We used a method where brain cortex was digested by trypsin and DNase. The brain cortex was then placed on PDL-coated flasks for four hours at 37°C. The subtypes were further investigated using flow cytometry, EdU staining and the CCK-8 assay.

While Rab6A can be found in neuronal tissues and microglia, it was not detected within oligodendrocytes and NG2 cell. Rab6A is found in GFAP+ astrocytes. The SOX9 and Aldh2L1 chemicals label astrocytes. It is striking that Rab6A+ structures can vary greatly from one astrocyte to the next, suggesting that there is a dynamic regulation of glial GGN.

When synapses activate, gliotransmitters are released by astrocytes. In this model, astrocytes release glutamate in response an electric stimulus. This transmitter is released by nearby synapses. The researchers measured the frequency of the mEPSC and found that both TeNTLC as well as BAPTA blocked this release. Non-functional TeNTLC was not able to block the astrocyte-evoked release glutamate. The time required for this process was brief, and the astrocyte evoked a synaptic effect was mediated via both SNAREs as well as Ca2+.

SNAPs are involved in synaptic function and regulation. In neurons, regulated exocytosis is triggered by action potentials (APs) at the nerve terminals. This fusion is made possible by the interaction of synaptotagmin 1, Syntaxin 1, VAMP2, and synaptotagmin 2. Although it has been suggested that astrocytes may be involved in regulated exocytosis (the mechanism that triggers this process is not yet known),

It is important that astrocytes have low levels of glutamate, which could cause problems with gliotransmission. GABAA-receptor expression can also be affected if there is insufficient glutamate. It is important to research the role of glutamate inside astrocytes. So, it is clear that astrocytes are a key component of gliotransmission.

Blot in the West

A Western blot using RAB6a markers should be specific for the protein being analysed. While Rabphilin-3A is very similar in terms expression and molecular mass, they differ in their amino-acid sequences. These antibodies generally produce similar results on Western blots. This could be because Rabphilin-3A may have a different structure from Rab6A but they share similar functions.

In a sandwich experiment we used rabbit polyclonal and mouse monoclonal myc antibodies (Bethyl) to test for GFP. Secondary antibodies were either conjugated to IRDye 680, or IRDye800 and blots were scanned with the LI-COR Odyssey scanner. To identify the protein targets, we used the following immunoblots.

HEK293T cells were transiently co-transfected with either myc-tagged Rab6a or GFP-dynactin-6. GFP was transfected to a negative control. After transfection, E.coliDH5a cells were grown at 37°C to an OD600 value of 0.3-0.5. After incubation with 0.1 mM isopropyl-1-galactopyranoside for 18h at 30degC, cells were lysed by sonication. The samples were then incubated with 0.1 mM isopropyl-1-galactopyranoside for 18 hours at 30degC. Cells were then lysed by sonication.

Although Rab6A is a common MOLECULAR SWITCH between the ER & Golgi molecular switches, it is important for you to know that its cloned cousin, Rab6A, is specific to the Golgi as well as anterograde transport. Rab6a Q72L induces significant Oglycosylation in its invariant chain. Rab6a's dominant negative form hinders the continuous recycling of resident proteins from Golgi into ER.

Methods of detection

The RAB6A protein belongs to the small GTPase group. It has been implicated in the regulation of membrane traffic from the Golgi apparatus to the ER. It is expressed in astrocytes of various organisms and has low GTPase activity. This recombinant protein from human RAB6A was created in E.coli, and purified using standard chromatography techniques. A 3 month warranty is provided for clones of molecular origin.

Multiple detection methods have been developed to detect the presence or absence of this protein in different cell types. Recently, a study by Chen et al. (16) showed that Rab7 and the Lamp1 protein are co-localized in vesicles escaping the TGN. Although the study did not look for Rab7, it revealed a heterogeneous population TfR-containing Vesicles that contained either Lamp1 and Rab7. M6PR can also be used as a marker for the Golgi to-endosome route.

RA patients can be identified using molecular genetic analysis. The RAB6A genetic locus is on chromosome 13.4. Multiple genes found in this region have been linked to RA, including FLJ11848 and MPRL48. Further analysis of the region will be required using higher density SNP marks. The present invention has many advantages and is applicable to other complicated diseases. This discovery presents a unique opportunity to detect RA accurately and in a novel way.

Rab6A monoclonal antibodies have been developed for detection of this protein. This antibody is raised against the C-terminus of the Rab6A protein. It has been used before to replicate perinuclear stains in CHO cells. It is not clear that Rab6A monoclonal antibodies are specific for Rab7 in blood. However, the RAB6A antibody marker may be specific to the same molecule in two separate cell lines.