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- Table of Contents
Facts about Rab3 GTPase-activating protein non-catalytic subunit.
Rab3 GTPase-activating complex especially converts active Rab3-GTP into the inactive form Rab3- GDP. Required for normal eye and brain development.
Human | |
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Gene Name: | RAB3GAP2 |
Uniprot: | Q9H2M9 |
Entrez: | 25782 |
Belongs to: |
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Rab3-GAP regulatory subunit family |
DKFZP434D245; FLJ14579; KIAA0839p150; RAB3 GTPase activating protein subunit 2 (non-catalytic); Rab3 GTPase-activating protein 150 kDa subunit; rab3 GTPase-activating protein non-catalytic subunit; Rab3-GAP p150; Rab3-GAP regulatory subunit; RAB3GAP150; RAB3-GAP150; RGAP-iso
Mass (kDA):
155.985 kDA
Human | |
---|---|
Location: | 1q41 |
Sequence: | 1; NC_000001.11 (220148293..220272453, complement) |
Ubiquitous.
Cytoplasm. In neurons, it is enriched in the synaptic soluble fraction.
This article will explain the history and primary antibodies of Steven Boster. It will also show how to use His RAB3GAP2 antibody for Western blots. The RAB3GAP2 marker is a highly-respected target for flow cytometry. It is a small, soluble protein. It has many applications in science, from cancer research to vascular diseases.
Steven Boster died June 26, 2022. He was born in Joliet Illinois. Evelyn Meier Boster, James Meier Boster, were his parents. He was a veteran retail manager. He served in the U.S. Army and was a member of Concordia Hall in Staunton, VA. Steve is survived by 2 Daughters - Natosha Peck and Crystal Boster - and 6 Grandchildren. He is also survived and cared for by his brothers Jack Boster and Sandra Blanton, as well as his sisters Tammy & Lisa Milton. Jonathan and Cory are his children. Many nieces and nephews have been blessed by his presence.
Steve was proudest of his family. He loved to sing in his lower register in front his family. He was also a passionate fan of auto racing and sports. He would often rescue his car when it broke down at 2 am. He was a faithful friend and showed up to appointments at track in below freezing temperatures. His generosity extended to all of his friends. He treated everyone as a member of his family.
The RAB3GAP2 marker, an immunoglobulin that has high affinity for actin, is known as an immunoglobulin. Steven Boster developed this monoclonal antibody. This product is well-validated for ELISA, immunohistochemistry, and Western blotting. Boster can also combine this antibody using a variety of conjugates. This will allow you to achieve the highest signal-to noise ratio.
The RAB3GAP2 gene provides instructions to make a protein, RAB3GAP2. This protein regulates GTPases, an enzyme that controls hormones and other brain chemicals. The RAB3GAP complex includes the RAB3GAP1 proteins. RAB3GAP2 & RAB3GAP1 work together to regulate many cellular processes.
Boster Bio is a market leader in Western blot antigen detection. Its products are used by biochemical researchers to find and identify specific proteins in biological samples. Antigens are foreign substances that cause antibodies to be produced by the body. Antibodies can recognize target proteins that are immobilized on a membrane in a variety of ways. They can target specific parts or modified forms of the protein.
Steven Xia was the founder of the company. He specializes on developing high-specificity antibodies and ELISA reagents. Boster Bio has published over 18,000 papers since its inception. The products have been transparently tested for multiple applications such as Western blot or flow cytometry. Boster Bio has a dedicated team that works diligently to ensure its products meet high quality standards and are compatible for many protocols. This is in order to provide outstanding customer service. Boster Bio's products are continually expanded to meet changing customer needs.
The primary antibody is attached to a tag to determine whether a protein is present. The type of secondary antibody used in western blotting depends on how well they can bind to the target proteins. Two of the most popular methods for western blot detection include fluorescent and enzymatic chemical chemiluminescence. These methods require the use a reagent with a fluorescent substance. They can be used to detect a particular protein in biological samples.
The process for western blot antibody detection is very much the same as that used in immunoassays. Gel electrophoresis is the first step, which separates soluble protein into discrete bands. The sample proteins are then transferred onto a membrane matrix (nitrocellulose or PVDF). These proteins are then stained by an antibody probe specific towards the target protein antigens. The secondary antibody reacts with the enzyme to produce a detectable signal.
PMID: 9733780 by Nagano F., et al. Molecular cloning and characterization of the noncatalytic subunit of the Rab3 subfamily-specific GTPase-activating protein.
PMID: 22337587 by Haines D.S., et al. Protein interaction profiling of the p97 adaptor UBXD1 points to a role for the complex in modulating ERGIC-53 trafficking.