tRNA pseudouridine synthase A (Pus1)

Converts specific uridines to PSI in several of tRNA substrates. Acts on places 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed:10094309).

Involved in regulation of nuclear receptor action possibly through pseudouridylation of SRA1 RNA (PubMed:15327771). Isoforms 3 and 4 don't form pseudouridine when expressed in vitro (PubMed:11085940).