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- Table of Contents
Facts about 26S proteasome regulatory subunit 6A.
Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC3 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are simultaneously translocated into a proteolytic room and degraded into peptides.
Human | |
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Gene Name: | PSMC3 |
Uniprot: | P17980 |
Entrez: | 5702 |
Belongs to: |
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AAA ATPase family |
human immunodeficiency virus tat transactivator binding protein-1; MGC8487; proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5; Proteasome 26S subunit ATPase 3; Proteasome subunit P50; TBP-126S protease regulatory subunit 6A; TBP1Tat-binding protein 1
Mass (kDA):
49.204 kDA
Human | |
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Location: | 11p11.2 |
Sequence: | 11; NC_000011.10 (47418769..47426473, complement) |
Cytoplasm. Nucleus. Colocalizes with TRIM5 in the cytoplasmic bodies.
This one is the best choice for boster biomarker and other tests. Boster's high-affinity primary antibodies and chemiluminescent detection devices are excellent options for this test. This article will cover how to use these products incubation and recording results on autoradiography films. You can also read our previous articles about the Boster products.
Boster's chemiluminescence detection system for PSMC3 was developed to identify PSMC3 in samples. PSMC3 was detected in both patient cells and control cells. The mRNA also appeared in a reduced amount. Although the protein does not exist in a stable form it is important to detect it with Boster's PSMC3-chemiluminescent detection systems.
This test uses antibodies that have been validated on multiple platforms with known positive and false samples. Boster also awards product credits to scientists that review products early. These awards are given to scientists who discover new uses for existing products and develop new antibodies. The Boster Chemiluminescent Detection Systems for PSMC3 have the highest sensitivity and specificity for this protein detection.
The PSMC3 gene encodes the protein with an AAA domain at the C terminus. It is a member of the heterohexameric ring of AAA proteins. It plays an important role in maintaining cellular protein homeostasis by removing misfolded, damaged, or no longer required proteins. This enzyme also plays a role in cell cycle progression, apoptosis and DNA damage repair.
The PSMC3 mass assay resulted in the discovery that the pathogenic variant impacted the ubiquitin–proteasome pathway. The PSMC3 was able to hydrolyze Suc LLVY fluorogenic Peptide, allowing the detection and identification of proteasome-related bands. The two bands visible in x-axis corresponded with the 20S and the 26S proteasome complexes. The two bands below corresponded the respective 19S, 20S precursor intermediates.
The PSMC3 is an effective determinant for cell identity and is a phosphorylated, polypeptide. This marker is used in a number of diagnostic applications and can be used in immunoprecipitation, flow cytometry, and other research applications. Primary antibodies are the most frequently used in diagnostic and fundamental research. They are essential in identifying disease-causing organisms in the body.
The PSMC3 markers are a good candidate for high-affinity primary antibody production. They can detect the protein of particular interest. This marker is also highly resistant against heat. It is stable at higher concentrations and can withstand high temperatures. In addition, the antibodies can withstand a wide range of temperatures. The PSMC3 marker can be used to identify a variety of antigens in immunohistochemistry.
These high-affinity primary antibody use additional markers than the PSMC3 marker. There are also goat and rabbit anti-llama antibody options. Capralogics and Cayman Chemical Company both manufacture antibodies and assays that can be used in various research applications. Cedarlane Laboratories sells monoclonal, and polyclonal, antibodies. The company also offers secondary and primary reagents derived from avian cells. The company also offers Isotype Controls and Isotype Control Kits.
The PSMC3 marker is used to validate the high-affinity primary antibodies of Boster. The PSMC3 marker has been successfully used to detect high-affinity primaries antibodies in the past and is highly specific. Boster can also use this marker to perform high-affinity immunopaque testing. The PSMC3 marker allows you to identify many disease-causing proteins.
PSMC3 (Proteasome Synthase), is a gene that helps with lens development and inner ear development. There are two types of the PSMC3 protein, one of which can be found in high numbers in patients. The mutation causes an increase of 20S or 26S proteasome structures in patient cells. Patients with this mutation have higher levels for ubiquitin and protein conjugates.
This gene is responsible for the unfolding of globular domains in the semicircular canal. It is a member from the AAA family. It consists of six distinct units. PSMC3 encodes Rpt5, which is an important component of substrate unfolding, and translocation. PSMC3 is implicated in the pathogenesis and progression of many diseases.
Mass spectrometry results for the co-immunoprecipitation of PSMC3 reveal that PSMC3 recruits the proteasome to the inner membrane. This recruitment leads to stabilization of HBx in an ubiquitin-independent manner. This mechanism could be used to develop future DUB inhibitors. PSMC3 can recruit subunits in many other ways.
The PSMC3 mutant is a noncoding and pathogenic variant. It is associated among other features with severe congenital and early cataracts. The PSMC3 gene encodes the 26S regulatory subunit 6A (AAA-ATPase). The proteasome-complex is a multiprotein protein complex that maintains cell protein homeostasis. It regulates enzyme activity in cells, including the ubiquitination proteins.
Autoradiography uses radioactive substances to take photographs of organs and tissues. The film will show the dark areas of the irradiated area, which corresponds to the distributions a tracer. It is a great method to imaging organs, tissue, and cells. The physician can monitor health problems before they become life-threatening. It is easy to record the test result with autoradiography film, but it requires special equipment. Also, the technical expertise of a skilled technician is required.
The amount of radioactivity in a tissue or organ determines the resolution of autoradiography. The detection system is a photomultiplier, which measures the intensity emitted light. Imaging technology has advanced to allow you to record the test result in real-time. Instead of imaging plates, gas detectors can detect a/b-particle fragmentation. These newer imaging techniques allow for high-speed, digital autoradiograms.
The film used in autoradiography is a combination of photographic and digital images. An emulsion of radiation sensitive crystals coated on transparent base material is the active component of a film. This material converts radiation from visible light into visible images in a fraction-of-a second. The amount of exposure required varies depending on the sensitivity, design and processing of autoradiography apparatus.
PMID: 8419915 by Ohana B., et al. The type 1 human immunodeficiency virus Tat binding protein is a transcriptional activator belonging to an additional family of evolutionarily conserved genes.
PMID: 2194290 by Nelbock P., et al. A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator.