Periplakin Antibodies

Periplakin (PPL)

Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments.

May act as a localization signal in PKB/AKT-mediated signaling. .

Gene Information

Human
Gene Name:	PPL
Uniprot:	O60437
Entrez:	5493

Family

Belongs to:
plakin or cytolinker family

Alternative Names

190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein; KIAA0568; MGC134872; periplakin

Molecular Weight

Mass (kDA):
204.747 kDA

Genomic Context

Human
Location:	16p13.3
Sequence:	16; NC_000016.10 (4882507..4937148, complement)

Tissue Specificity

Expressed in stratified squamous epithelia and in some other epithelia.

Subcellular Localizations

Cell junction, desmosome. Cytoplasm, cytoskeleton. Cell membrane. Nucleus. Mitochondrion. Associated with desmosomes and intermediate filaments.

Diseases

• Allergy
• Lung Diseases
• Lung Diseases, Obstructive
• Malignant Neoplasms
• Penicillin Allergy
• Chronic Obstructive Airway Disease
• Neoplasms
• Lymphoma
• Lung Neoplasms
• Cardiovascular Diseases
• Hypertensive Disease

• Polyendocrinopathies, Autoimmune
• Paraneoplastic Syndromes
• Tissue Adhesions
• Diabetes Mellitus
• Lung Injury
• Inflammation

Interacting Proteins

• AHCYL1
• AKT1
• ALAS1
• ANXA9
• BTN3A1

• RAB3IP
• RALBP1
• TRAF2
• TRIM27
• TSC22D4

• VIM
• ZSCAN1

Pathways

• Hypersensitivity
• Transport
• Localization
• Pathogenesis
• Secretion
• Coagulation
• Sensitization
• Cell Adhesion
• Reflex
• Anaphylaxis
• Innervation
• Muscle Contraction
• Inflammatory Response

• Cell Migration
• Lymphocyte Proliferation
• Regeneration
• Excretion
• Immune Response
• Cell Death
• Killer Activity

PTMs

• Phosphorylation
• Oxidation
• Cleavage
• Acylation
• Deacetylation
• Amination
• Acetylation

• Carboxylation
• Demethylation
• Dephosphorylation
• Iodination
• Reduction
• Glycosylation
• Palmitoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PPL

Best Uses Of The PPL Marker

A high-affinity primary antibody is one of the most important tools used in the research process, and Boster scientists can use this tool to submit their results. Boster scientists can submit results related to species, applications, or special samples and receive credits. These credits are available to all scientists worldwide. If you have been researching high-affinity primary antibodies but aren't sure where to begin, read on.

High-affinity primary antibodies

Boster Bio manufactures a variety of high-affinity primary antibodies for various applications in immunology, microbiology, molecular biology, and more. The company's antibodies are highly specific for the target protein and have been used in more than 25 years. These high-affinity antibodies have been tested for accuracy and sensitivity, and can detect hormones, small molecules, oxidative stress, inflammatory cytokines, and DNA damage. These reagents contain sufficient amounts of antibody for 96 tests and are available as a lyophilized preparation.

The ELISA technique requires the use of specific primary antibodies for the target antigen. These antibodies bind to the target antigen, and then a detection system signals that the antibody has bound to the antigen. To maximize the sensitivity of the assay, Boster Bio specializes in coating plates with high-affinity antibodies. Their reagents are used by scientists around the world to test their hypotheses.

Moreover, Boster offers a variety of rabbit mAbs with high affinity. The company uses its PCD platform to develop the best clones for the Diagnostics & Therapeutics market. This technology combines proprietary chemistry with flow cytometry to ensure that antibodies are retained on the membranes of B cells. The immune system of rabbits has historically generated a diverse range of high-affinity antibodies against non-immune antigens.

The primary-secondary antibody system offers dual labeling for specimens. Dual-labeling also allows researchers to ask more questions and get better contextual data. These advantages make it possible to perform many more applications with high-affinity primary antibodies. The versatility of these high-affinity antibodies makes them a perfect choice for biomedical research. With these products, researchers can make multiple experiments without sacrificing quality.

Using the correct controls is essential to ensuring the high-affinity of these antibodies. These controls help identify false positives and detect non-specific binding of the antibodies. For instance, an antibody should be validated by WB prior to use. Flow cytometry and IHC can be used to validate the specificity of the antibodies. As with all biochemical tests, the negative control is helpful in determining whether secondary antibodies are binding to the target protein unintentionally.

PPL Marker

In the past, markers were a niche tool used by graphic designers and manga artists. Because markers don't dry as fast as other media, they were largely avoided by serious artists. But since the rise of the internet, more artists have turned to markers as a cost-effective, convenient way to apply bold color quickly. Now, there are markers for every budget, style, and technique. Listed below are some of the most popular uses of the PPL Marker.

Redaction: A redaction marker pen works by applying pressure to the tip. Once the pen is pressed on the document, the ink will flow and appear as a red mark. Since the ink doesn't dry immediately, it takes several seconds for the ink to fully dry before it can be used again. The PPL Marker can also be used on shiny or glossy paper, such as prescription bottle stickers.

Multi-purpose: The PPL Marker's multiple-tip design allows you to use multiple tips at the same time. This versatility allows you to cover multiple labels with one marker and use many at once. A variety of sizes, shapes, and colors are available. And, because the PPL Marker has an ink color that doesn't run, it's great for labeling large areas. It is even compatible with a variety of different surfaces, such as glass and metal.

As for the PPL Marker, it's possible to use it as a screening tool for the general population. After all, the goal of any screening test is to detect cancer as early as possible, when it's still treatable. But, it's important to know that the PPL Marker doesn't eliminate all concerns. A positive result means a patient will have better treatment options, which can ultimately lead to a shorter life span and greater quality of life.

Artists' markers are another way to express their creativity. They're often referred to as art markers. Besides being versatile, artist alcohol markers are popular for calligraphy and other art forms. They don't bleed through the paper like other markers, but they don't blend as well as the PPL Marker. For these reasons, many artists and crafters prefer the alcohol-based markers.

Despite the low sensitivity of this inflammatory marker test, it's helpful for doctors to use it in certain situations. For example, if the patient has diabetes or hypertension, a positive result may indicate that the patient is infected with a specific disease. Likewise, an elevated level might indicate that someone is suffering from diabetes. The PPL Marker may also be an indicator of a more serious underlying condition, such as colon cancer.