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- Table of Contents
Facts about DNA polymerase alpha catalytic subunit.
The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are originally extended by the polymerase alpha catalytic subunit and then transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively.
Human | |
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Gene Name: | POLA1 |
Uniprot: | P09884 |
Entrez: | 5422 |
Belongs to: |
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DNA polymerase type-B family |
DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha catalytic subunit; DNA polymerase alpha p180 subunit; EC 2.7.7.7; p180; POLADKFZp686K1672; polymerase (DNA directed), alpha 1; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA directed), alpha; polymerase (DNA-directed), alpha (70kD)
Mass (kDA):
165.913 kDA
Human | |
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Location: | Xp22.11-p21.3 |
Sequence: | X; NC_000023.11 (24693877..24996986) |
Nucleus. Cytoplasm, cytosol. In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern.
If you're looking for the most sensitive POLA1 ELISA kits, look no further. These kits are based on the Picokine (tm) platform, which significantly increases sensitivity down to the picogram level. Additionally, these kits have been validated against a broad range of samples and are available for purchase. You can request a validation procedure and image to verify the results of the kit. Additionally, Picoband uses insights in immunogen design and technical support from BeNeLux distributor Sanbio.
The POLA1 gene is found on mouse X chromosome. Molecular and functional studies suggest that POLA1 has multiple roles in the cell. It was discovered that POLA1 colocalizes with DNA and RNA in a speckled pattern, which suggests that the gene is involved in DNA replication. However, this finding is only true in certain mouse strains.
Despite the numerous applications of this gene, the best way to utilize it is in the diagnosis of cancer. The POLA1 marker has been used in several studies, including a study by Starokadomskyy et al. in which this marker was found to colocalize with RNA and DNA in a speckled pattern. However, despite this finding, the POLA1 marker does not cause cancer cells to multiply abnormally.
Secondary antibodies can be used to determine which biomarker is the target of the antibody. Primary antibodies should be produced in a consistent manner and should not cross-react with other antibodies. They are also suitable for experiments involving denatured proteins, and are available in both purified and crude forms. Here are a few tips for selecting a primary antibody:
The 19711-1 AP antibody is a specific target of POLA1. The protein belongs to the DNA polymerase family, which plays an important role in initiation of DNA replication. POLA1 is a component of the DNA polymerase alpha complex, which consists of the catalytic subunit POLA1/p180 and the regulatory subunit POLA2/p70. POLA1 recruits itself to replication forks through interactions with the protein MCM10.
Dual secondary visualization of the same sample using mouse anti-GFAP and rat anti-NeuN showed less cross-species staining. Both primary and secondary antibodies competed for binding to the primary antibody, resulting in less astrocytic staining. Furthermore, sequential staining with rat anti-GFAP and mouse anti-NeuN antibodies revealed cross-species binding.
In order to find the right primary and secondary antibodies, it is necessary to know the type of antigens. Antibodies are produced by the immune system in response to a foreign substance. They have specificity for their cognate antigen and neutralize the toxins produced by microorganisms during infection. Antibodies are present in body fluids and make up about 20% of the protein in plasma. They are Y-shaped molecules and are made up of two identical heavy and light chains. They are joined by disulfide bonds.
PMID: 3359994 by Wong S.W., et al. Human DNA polymerase alpha gene expression is cell proliferation dependent and its primary structure is similar to both prokaryotic and eukaryotic replicative DNA polymerases.
PMID: 2005899 by Pearson B.E., et al. Human DNA polymerase alpha gene: sequences controlling expression in cycling and serum-stimulated cells.