Netrin-1 Antibodies

Netrin-1 (NTN1)

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with DCC or any UNC5 receptors will lead to axon attraction or repulsion, respectively.

It also serve as a survival factor via its association with its receptors that prevent the initiation of apoptosis. Involved in tumorigenesis by regulating apoptosis.

Gene Information

Human
Gene Name:	NTN1
Uniprot:	O95631
Entrez:	9423

Family

Belongs to:
No superfamily

Alternative Names

netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

Molecular Weight

Mass (kDA):
67.748 kDA

Genomic Context

Human
Location:	17p13.1
Sequence:	17; NC_000017.11 (9021510..9244000)

Subcellular Localizations

Secreted. Cytoplasm. Mainly secreted.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Colorectal Cancer
• Neoplasm Metastasis
• Hepatitis B
• Neoplasm Invasiveness
• Adenocarcinoma
• Liver Carcinoma
• Hepatitis C
• Prostatic Neoplasms
• Inflammation
• Nervousness

• Tissue Adhesions
• Malignant Neoplasm Of Breast
• Ataxia
• Hypoxia
• Dysplasia
• Tumor Angiogenesis
• Acute-phase Reaction

Interacting Proteins

• APP
• DCC
• DRAXIN

Pathways

• Cell Migration
• Axon Guidance
• Signal Transduction By P53 Class Mediator
• Cell Adhesion
• Negative Regulation Of Centriole Replication
• Activation Of Mapk Activity
• Cell Division
• Cell Motility
• Acute-phase Response
• Cell Differentiation
• Spinal Cord Development
• Regulation Of Wnt Receptor Signaling Pathway
• Cell Proliferation

• Neutrophil Chemotaxis
• Interleukin-8 Production
• Humoral Immune Response
• Regulation Of Peptidase Activity
• Dna Replication
• Cell Recognition
• Regulation Of Centriole Replication

PTMs

• Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NTN1

Best Uses of the NTN1 Marker

You must be aware of the best uses and limitations of Anti-Netrin 1/NTN1 antibodies before you purchase one. Here are some of our favorites:

Anti-Netrin 1/NTN1 Antibody

This Rabbit Polyclonal antibody recognises the Netrin-1 antigen in human, mouse, and rats. Boster Bio has validated the Western Blot use of this antibody. Netrin-1 can be used in many biological assays to measure gene expression in different cell types. It can inhibit apoptosis, and control cell guidance.

Netrin-1 levels have been found to be elevated in human senescent senescent cells. This is in line with the fact that SFs can be produced by senescent cell secretion of netrin-1. A qRTPCR experiment also demonstrated that netrin-1 blocking in MEF fibroblasts inhibited the formation of SFs in bleomycin induced premature senescents.

Stimulation of MST1 (MST1 proteins) increases the neutralization trap of NTN1. MST1 activates the UNC5B residue T428. This in turn phosphorylates UNC5B increasing cell death. NTN1 deprivation also increases UNC5B levels, possibly leading to apoptosis.

NTN1 decreases in PD brains. This is due to increased expressions of the UNC5B receptor and the Hippo pathway. These proteins regulate cell growth and control the development the nervous system. It is unclear how these proteins regulate Netrin1. The reduction of NTN1 stimulates MST1 Phosphoration of UNC5B. This is a netrin-receptor. By blocking MST1 activity, the expression of Netrin1 increases and neuronal apoptosis is suppressed.

In this study, netrin-1 expression was negatively regulated by EZH2 in human diploid fibroblasts. EZH2 binds to NTN1 promoters with greater efficiency in young 2BS cell lines, whereas EZH2 expression in senescent fibroblasts is lower. Similarly, Netrin-1 levels are negatively affected by EZH2 expression in human colon adenoma tissues.

Commercial ELISA kits

NTN1 ELISA kits commercially available are sandwich enzyme immuneassays (ELISAs), which can be used for quantitative measurement in human serum, plasma and tissue homogenates. Unlike other ELISA kits, which have a limited shelf life, LSBio ELISA kits are assembled on demand and tested before shipping. There may be minor changes in the range, sensitivity or precision. These are usually reported to the customer before shipping. Lot numbers will show the date of final assembly and testing as well as shipping.

Ntn1 ELISA kits are generally designed to detect a specific concentration of Netrin1 in biological samples. These kits are sensitive, specific, and should only be used under the appropriate storage conditions. It is crucial to use the exact same assay protocol for the sample to ensure high accuracy. A diluted sample may give results that are slightly lower or higher than you expected. This protein can be measured in biological samples using commercial NTN1 ELISA kit. They are convenient and inexpensive.

Trinity Biotech's ELISA kit commercial was deemed to be the best for epidemiological uses. Its OD values in endemic settings were highest for the Trinity Biotech kit. All commercial kits were able to detect all four human malaria species, though the sensitivity of some of them was questioned. However, the performance of commercial kits using DBS samples is still to be determined. Future work should concentrate on technological refinements, as well as outstanding programmatic issues.

To use a commercial NTN1 ELISA kit, first determine your sample concentrations. To get an accurate result, ensure you use a proper sample dilution. Make sure to do duplicate tests and compare the results. Then determine the optimum concentrations. Once you have determined optimum dilutions of the samples, you can then perform an assay. The percentage will be reported.

IHC-optimized polyclonal antibodies

The development of IHC-optimized polyclonals antibodies using the NTN1 marker is an exciting new way to detect a variety of proteins and antigens in tissue samples. This technique relies on specific antigens and antibodies to recognize different cell types and pathological circumstances. IHC staining takes place by incubating the antibodies with targeted tissue at a particular temperature and duration. Antibodies with lower concentrations will be more likely to bind the same epitopes. The type of target tissue will determine the incubation time. However, prolonged incubation times can result in non-specific signals.

IHC is widely used to diagnose disease, conduct biological research, develop drugs, and detect tumors. Monoclonal and polyclonal antibodies are less stable than polyclonal, which can be affected due to protein conformation, interactions, pH, fixation, salt concentration, and other proteins. These characteristics of polyclonal antibodies make them better for IHC studies, but monoclonal antibodies are still a preferred choice for certain applications.

IHC-optimized monoclonals against the NTN1 marker detect P21 in FFPE tissue samples. P21 is an antibody that is found in a human colon carcinoma section using a FFPE dye. This method has been used over decades and has resulted in the development of a reliable, IHC-optimized, polyclonal antibody that is p21-based.

The most important step in the entire process is choosing a primary antibody to perform an IHC/ICC experiment. Primary antibodies must be chosen based upon their specificity for an epitope. All steps of an experiment should be optimised to minimize background signals and visualize specific staining. The working dilution of polyclonal antibodies is generally lower than that of monoclonal antibodies, but must be determined empirically. High-quality antibodies must have minimal cross-reactivity.

Picogram sensitivity ELISA Kits

The use of Picokine ELISA kits for the quantification of low levels of human C-peptide in plasma and serum has numerous benefits. These kits can be used for research purposes because the biomarker has an insulin-like half-life. It serves as a good measure for insulin production in beta-cells. In addition, the Picokine ELISA kits are highly sensitive and specific.