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Facts about Neuronal PAS domain-containing protein 2.
It is derived from the Latin roots 'circa' (about) and 'diem' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system.
Human | |
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Gene Name: | NPAS2 |
Uniprot: | Q99743 |
Entrez: | 4862 |
Belongs to: |
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No superfamily |
Basic-helix-loop-helix-PAS protein MOP4; BHLHE9; bHLHe9MGC71151; Class E basic helix-loop-helix protein 9; Member of PAS protein 4; member of PAS superfamily 4; MOP4PAS domain-containing protein 4; neuronal PAS domain protein 2; neuronal PAS domain-containing protein 2; Neuronal PAS2; PASD4FLJ23138
Mass (kDA):
91.791 kDA
Human | |
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Location: | 2q11.2 |
Sequence: | 2; NC_000002.12 (100820139..100996829) |
Nucleus.
Boster Bio can assist with antibodies that are used to detect NPAS2. It offers high-affinity primaries. Before you start, there are a few things you should consider. These factors can impact the results of your experiment. Listed below are some optimization tips for Boster Bio. You can also read Steven Boster's story. He's an entrepreneur, with a proven track record.
It can be difficult to optimize IHC experiments using NPAS2 marker. There are so many variables, so it is helpful to know the ins and outs of flow cytometry. Boster Bio provides useful tips and information about the NPAS2 IHC marker. Flow cytometry uses laser-based technology to profile cells within a mixture of heterogeneous liquids. FACS is another term for this technique. Boster Bio offers primers and protocols to help users navigate this process. Flow cytometry is another popular method. Download the FACS product datasheet NPAS2 marker.
Here are some facts to help you understand the life of Steve Boster. Steve loved the southern gospel music and sang in a lower register in public. He enjoyed auto racing, sports, and other activities. He was the one who called when his car broke down at two o'clock in the morning. He also showed up on time, even in below freezing conditions. Steve was a true family man and treated everyone as family.
He was born in Joliet in Illinois and he died in Madison in WI after a long battle for COVID-19. He was the father to Donald, Sr., David Boster, as well as Nina Mae Hall. His two daughters, Crystal Boster, and Natosha, are his surviving siblings. He also has 6 grandchildren and 4 brothers, Jack Boster, Sandra Blanton, and 6 great-grandchildren. His sister Tammy and her son Jonathan are among his other relatives.
We used the NPAS2 mark to develop a new method of determining high-affinity primary antibody. This method can identify and distinguish new types of antibody. High-affinity primaries have a high sensitivity to insulin. The NPAS2 marker is found in many tissues including blood. This marker has been shown to be a reliable and useful tool in the identification of novel antibodies.
To generate these high-affinity antibodies, the NPAS2 gene was first identified. It is an important gene that is found in all human cells. It is responsible bind to foreign proteins including antigens. The NPAS2 genome encodes a polyvalent peptide. Both polypeptides can be binded by antibodies that are able to recognize NP-derived polypeptides.
The NPAS2 gene is a basic-helix-loop-helix transcription factor that functions as the functional ortholog of CLOCK. The hypothalamic circadian rhythm is governed by the CLOCK-BMAL1 dimer. Fibrosis in mice with Clock null or Bmal1 mutations is associated with abnormal bone metabolism. It has been shown to be associated with increased NPAS2 and abnormal scar formation.
The mouse model was used for studying the relationship between IgD2 and NPAS2. IgD deficiency in mice inhibits the immune response to autoantigens, and induces affinity maturation. IgD-deficient mice have insulin-reactive IgM levels that are higher in WT mice than in IgD-deficient ones. The mice developed diabetes in both the WT and IgD-deficient groups. They were able to produce insulin-specific antibodies that showed high affinity for insulin.
After analysing HTS data set obtained from two assays and evaluating the NPAS2 indicator, We also investigated the NPAS2-LacZ gene expression. Dwn1 is a candidate compound to be used in further experiments. These results highlight the importance and value of the NPAS2 marker for high-affinity primary antibody.
We used the InsA derived from mice for the insulin specific IgM (IgM). We then loaded these antibodies onto Streptavidin-based biosensors and measured the binding affinities in nm. The separations were then done using a protocol using SDS–PAGE and Coomassie stained. After separation, we used ELISA to verify the purity and specificity of the insulin-specific antibodies.
PMID: 9012850 by Zhou Y.-D., et al. Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system.
PMID: 9079689 by Hogenesch J.B., et al. Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway.