Nidogen-1 Antibodies

Nidogen-1 (NID1)

Sulfated glycoprotein widely distributed in basement membranes and tightly connected with laminin. Also binds to collagen IV and perlecan.

It probably has a role in cell- extracellular matrix interactions. .

Gene Information

Human
Gene Name:	NID1
Uniprot:	P14543
Entrez:	4811

Family

Belongs to:
No superfamily

Alternative Names

enactin; Entactin; Entactin-1; NID1; NID-1; NIDentactin; nidogen (enactin); nidogen 1; Nidogen1; Nidogen-1

Molecular Weight

Mass (kDA):
136.377 kDA

Genomic Context

Human
Location:	1q42.3
Sequence:	1; NC_000001.11 (235975830..236065092, complement)

Subcellular Localizations

Secreted, extracellular space, extracellular matrix, basement membrane.

Diseases

• Malignant Neoplasms
• Neoplasms
• Cell Invasion
• Neoplasm Metastasis
• Malignant Neoplasm Of Ovary
• Malignant Paraganglionic Neoplasm
• Tissue Adhesions
• Cholangiocarcinoma
• Melanoma
• Malignant Tumor Of Colon
• Gastrointestinal Neoplasms
• Neoplasm Invasiveness
• Malignant Neoplasm Of Lung

• Tumor Angiogenesis
• Lupus Erythematosus, Systemic
• Malignant Disease (disorder)
• Epithelial Ovarian Cancer
• Ovarian Neoplasm
• Malignant Neoplasm Of Gastrointestinal Tract
• Cell Transformation, Neoplastic

Pathways

• Cell Migration
• Cell Adhesion
• Long-term Synaptic Potentiation
• Vasculature Development
• Proteolysis
• Regulation Of Gene Expression
• Demethylation
• Cell Maturation
• Methylation
• Cell Division

• Gene Conversion
• Chemotaxis
• Cell Motility
• Protein Folding
• Angiogenesis

PTMs

• Demethylation

• Cleavage

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NID1

Boster Bio - Best Uses Of The NID1 Marker

In this article, we will look at the informationgraphics Boster has created for the NID1 marker, how to validate an ELISA kit and the sample type for tissue homogenates. These guidelines can help optimize the quality of NID1 gene analyses. Please contact us with any questions. We'd be happy to help with determining the best type of sample and protocol for your research.

ELISA kit validation

An ELISA is an enzyme-linked immunosorbent assay, a highly versatile method of detecting peptides, proteins, antibodies, and hormones. The technique can yield quantitative data and is extremely convenient to perform. However, not all ELISA kits can be used in the same way. Here are a few factors to consider when purchasing an ELISA kit. There are 4 types of ELISA kit: one for each type, the sandwich, the custom, and the quantitative.

A validated ELISA kit has undergone extensive cross-validation. It can detect antibodies from serum and plasma, cell lysate and tissue. It is important to have a thorough validation procedure in place in order to avoid false positives caused by interference from unrelated molecules or cross-reactivity. Before an ELISA can be used, it must first be tested against the specimen. Boster Bio NID1 ELISA kits validation can be requested.

Tissue homogenates used as a representative sample type

Tissue homogenates is prepared by mechanically destroying fresh tissue. The cells are stripped from blood and the cell membranes of the permeabilized. Homogenized tissue has several advantages over other preparations. Tissue homogenates are particularly useful for studying the dynamics mt-respiration. Tissue homogenates also have a lower heterogeneity than other types of samples, which minimizes problems associated with oxygen limitation.

In mice, tissue homogenates were used to detect NID1 mRNA in interstitial cells and the outer layer of theca cells. Expression of Aspn/PLAP-1 could only be found in the outer layer, and not in the inner. This indicates that Aspn/PLAP-1 expression is only found in these cells, and not in other tissues.