NFAT5 Antibodies

Nuclear factor of activated T-cells 5 (NFAT5)

Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed:10051678).

Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the existence of DDX5/DDX17 (PubMed:22266867). Binds the DNA consensus sequence 5'- [ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed:10377394).

Gene Information

Human
Gene Name:	NFAT5
Uniprot:	O94916
Entrez:	10725

Family

Belongs to:
No superfamily

Alternative Names

EC 3.6.1; EC 3.6.3.14; KIAA0827glutamine rich protein H65; NF-AT5NFAT-like protein 1; NFATL1; NFATZ; nuclear factor of activated T-cells 5, tonicity-responsive; OREBP; osmotic response element-binding protein; T-cell transcription factor NFAT5; TonE-binding protein; TonEBP; TONEBPnuclear factor of activated T-cells 5; Tonicity-responsive enhancer-binding protein

Molecular Weight

Mass (kDA):
165.763 kDA

Genomic Context

Human
Location:	16q22.1
Sequence:	16; NC_000016.10 (69565952..69704654)

Tissue Specificity

Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes.

Subcellular Localizations

Nucleus. Cytoplasm. Nuclear distribution increases under hypertonic conditions.

Diseases

• Disease Of Adrenal Medulla
• Malignant Neoplasms
• Physiological Stress
• Dehydration
• Neoplasms
• Carcinoma
• Cell Invasion
• Hypoxia
• Neoplasm Metastasis
• Deprivation Of Water
• Mammary Neoplasms
• Diabetes Mellitus

• Inflammation
• Hypernatremia
• Edema
• Anti-diuresis (finding)
• Hyperosmolality
• Immunologic Deficiency Syndromes
• Liver Carcinoma

Interacting Proteins

• DDX17
• DDX5
• PTPN6

Pathways

• Localization
• Cell Death
• Transport
• Response To Osmotic Stress
• Cell Proliferation
• Rna Interference
• Cell Migration
• Nuclear Export
• Cell Cycle
• Nuclear Import
• Cell Growth
• Cell Motility
• Immune Response

• Methylation
• Cytokine Production
• Water Homeostasis
• Secretion
• Cell Activation
• T Cell Activation
• Cell Differentiation

PTMs

• Phosphorylation
• Acetylation
• Dephosphorylation

• Methylation
• Demethylation
• Nitrosylation

• Glycosylation

Research Areas

• Wnt Signaling Pathway

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NFAT5

Best Uses Of The NFAT5 Marker

This Boster Bio article will cover the NFAT5 Marker, Steven's history, and the different interactions the NFAT5 marker has. It will also cover the most common uses for this marker. You can also find tips and guides for optimizing your experiment. There are many options and choices for flow procedures. If you're not sure what to choose, check out our optimization guides and FAQs for more information.

NFAT5 Marker

Antibodies to the NFAT5 marker are used in many biological assays. They are polyclonal or monoclonal. Boster Bio develops antibodies against NFAT5 using rabbit and mouse as models. NFAT5 is a nuclear transcription factor that positively regulates the expression of S100A4 and LCN2. DDX5 and DDX17 are required for optimal transactivation of these genes.

The NFAT5 gene encodes a protein called Tonicity-Response Enhancer Binding Protein (TREBP), which stimulates transcription in response to hypertonicity. It is a subunit of the NFAT family of proteins that are found in every cell type. It is a nuclear NFAT protein that does not cooperate with Fos or Jun.

Steven Boster's history

Steve Boster's history is dotted with historical landmarks, including his current home address, past addresses, email addresses, and known relatives. The information can be filtered based on Steve's age and state of residence. If you are curious about Steve's history, you can read about the many ways he made a name for himself. Among his many contributions are the creation of the PicoKine(tm) ELISA platform. Using this proprietary platform, Steve developed ELISA kits that are highly sensitive and accurate.

Steve Boster was born on June 6, 1952 in Joliet, IL. He had spent many years in retail sales, and was a veteran of the U.S. Army. He was also a member of Concordia Hall in Staunton, VA. His family includes two Daughters, Natosha Peck and Crystal Boster, six grandchildren, and 4 brothers, Jack Boster, Sandra Blanton, and Tammy Boster. His nieces and nephews include Aaron Boster, Andrew Boster, and Joshua Peck.

Interactions with NFAT5 Marker

Antibodies to NFAT5 are used in many biological assays. They can be either polyclonal or monoclonal. Boster Bio develops antibodies to NFAT5 in mice and rabbits. This NFAT5 molecule is responsible for positively regulating the transcription of two genes, LCN2 and S100A4. It also interacts with DDX5/DDX17, a nuclear-localized kinase that facilitates transactivation.

NF-kB p65 binds to a site upstream of the Nfat5 gene. The binding site is located 3000 to +1 base pairs from the Nfat5 exon. Mutant DNA oligonucleotides and double-stranded oligonucleotides contain this binding site. Using KRN2 as an inhibitor of NF-kB p65 reduced the binding capacity of the NF-kB p65 to the Nfat5 promoter.

Nuclear factor of activated T cells 5 (NFAT5) is implicated in various diseases. Currently, there are no therapeutic agents that block the activity of NFAT5. A large library of chemicals was screened using a cell-based assay to identify NFAT5 inhibitors. One such inhibitor, KRN2, inhibits NFAT5 upregulation in macrophages stimulated with lipopolysaccharide, and suppresses the formation of NF-kB p65-DNA complexes in the NFAT5 promoter region.

The NFAT5 protein is an inducible nuclear factor that binds to antigen-receptor response element-2 (ARRE-2) of human T cells. It is involved in cell proliferation, survival, invasion, migration, and angiogenesis. Some cancers express constitutive levels of NFAT5, and its crystal structure is conserved. For this reason, it is an essential part of the immune system.