Midkine Antibodies

Midkine (MDK)

Developmentally controlled, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation.

Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland growth (By similarity).

Gene Information

Human
Gene Name:	MDK
Uniprot:	P21741
Entrez:	4192

Family

Belongs to:
pleiotrophin family

Alternative Names

Amphiregulin-associated protein; ARAP; MDK; MEK; Midgestation and kidney protein; midkine (neurite growth-promoting factor 2); Midkine; MK1; MKARAP; NEGF2; NEGF2FLJ27379; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

Molecular Weight

Mass (kDA):
15.585 kDA

Genomic Context

Human
Location:	11p11.2
Sequence:	11; NC_000011.10 (46380784..46383837)

Tissue Specificity

Expressed in various tumor cell lines. In insulinoma tissue predominantly expressed in precancerous lesions.

Subcellular Localizations

Secreted.

Diseases

• Malignant Neoplasms
• Neoplasms
• Carcinoma
• Malignant Paraganglionic Neoplasm
• Dysplasia
• Tumor Angiogenesis
• Inflammation
• Kidney Diseases
• Neoplasm Metastasis
• Multicystic Dysplastic Kidney
• Mental Disorders
• Tissue Adhesions

• Acute Lymphocytic Leukemia
• Ischemia
• Cell Transformation, Neoplastic
• Pathologic Neovascularization
• Renal Dysplasia
• Malignant Neoplasm Of Stomach

Pathways

• Angiogenesis
• Pathogenesis
• Cell Proliferation
• Cell Growth
• Cell Migration
• Localization
• Neurogenesis
• Cell Cycle
• Regulation Of Growth
• Regeneration
• Drug Resistance
• Lung Development
• Cell Division

• Dna Methylation
• Chemotaxis
• Methylation
• Cell Death
• Viral Replication
• Cell Adhesion
• Osteoblast Differentiation

PTMs

• Phosphorylation

• Methylation

• Ribosylation

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MDK

Boster Bio - Best Uses Of The MDK Marker

Boster scientists have access to various tools for scientific analysis. In addition to submitting data for species, applications, and specific samples, scientists also receive product credits. This benefits scientists around the globe. However, there are things to be aware of. Read on to learn about these features and how you can benefit from these features. Also, find out the ways Boster scientists use this device. Also, keep reading for some helpful tips and tricks to maximising the MDK Marker.

Optimization

Methods of molecular biology are widely used in the scientific community to manipulate and study of genetic material. Boster offers a broad range of technical information, including blogs, disease information , and web-based digital tools. Additionally, their top-quality buffers for lysis are designed to limit the amount of cross-linking to guarantee high-quality results. Boster's top-quality lysis buffers reduce the intensity of cross-linking to guarantee superior results.

Benefits

The MDK marker is a urine protein that is single, is highly specific for Bca. This marker is a good alternative for detecting the cancer in patients who do not have macrohematuria. Multiplex tests are more expensive, however MDK is one urine protein that is sensitive against Bca. MDK provides many advantages. It is not a diagnostic test, but can be used to detect Bca.

One study revealed that the MDK marker was highly expressed in Bca tissues and was correlated with Ki67. This was not seen in low-grade Bca tissues, or tumors with a higher grade. The same researchers also observed a positive correlation between the MDK protein and Ki67, suggesting that the MDK marker may be an indicator for this disease. This study is ongoing and warrants further study.

Another study showed mice deficient in the MDK marker had a lower loss of brain tissue and improved neurological deficits. The ratio of Iba1-immunoreactive microglia macrophages and glial fibrillary acidic proteins were comparable in Mdk-/ and+ mice. The M1 marker for phenotypes, CD16/32, was lower in Mdk+/+ mice.

Refer to

One method to test MDK levels in human blood is through an immunoadsorption assay. This test can identify the human version of MDK. Another test is based on the tumor-driven MDK effects. This latter test is especially useful in studies involving tumors because it can distinguish both effects of tumors that are caused by MDK and also the MDK that originates from nontumor-related cells.

Several references to the MDK marker are available. They are available in many formats and include protein-protein interactions, kinase assays, and other analytical techniques. Boster has a variety of primary antibodies that can be used in research. They are high-affinity, standardized, and widely-used. These antibodies can also be used in immunohistochemistry, Western Blotting, and ELISA.