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MLH1 Antibodies
AND MLH1 ELISA kits, MLH1 Proteins
Many biological assays use antibodies to detect MLH1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with MLH1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop MLH1 antibodies...
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MLH1 Infographic by Boster Bio
All MLH1 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # M00126
100 μl
Cat # M00126
100 μl
MLH1 Overview
Facts about DNA mismatch repair protein Mlh1.
DNA mismatch repair protein Mlh1 (MLH1)
Heterodimerizes using PMS2 to form MutL alpha, a part of this post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex.
Meeting of this MutL-MutS- heteroduplex ternary complex in existence of RFC and PCNA is enough to trigger endonuclease activity of PMS2. It introduces single-strand breaks close to the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.
Gene Information
| Human | |
|---|---|
| Gene Name: | MLH1 |
| Uniprot: | P40692 |
| Entrez: | 4292 |
Family
| Belongs to: |
|---|
| DNA mismatch repair MutL/HexB family |
Alternative Names
COCA2FCC2; DNA mismatch repair protein Mlh1; hMLH1; HNPCC; HNPCC2MGC5172; mutL (E. coli) homolog 1 (colon cancer, nonpolyposis type 2); mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli); MutL protein homolog 1
Molecular Weight
Mass (kDA):
84.601 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3p22.2 |
| Sequence: | 3; NC_000003.12 (36993350..37050846) |
Tissue Specificity
Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
Subcellular Localizations
Nucleus. Chromosome. Recruited to chromatin in a MCM9-dependent manner.
Diseases
- Malignant Neoplasms
- Neoplasms
- Colorectal Cancer
- Microsatellite Instability
- Hereditary Nonpolyposis Colorectal Cancer
- Colorectal Neoplasms
- Carcinoma
- Malignant Tumor Of Colon
- Adenocarcinoma
- Endometrial Polyp
- Endometrial Carcinoma
- Carcinogenesis
Interacting Proteins
- ACTG1
- ANXA2
- BRIP1
- CTSB
- DES
- FAM208B
- FSCN1
- KPNA2
- MAGEA8
- MSH3
Pathways
- Mismatch Repair
- Methylation
- Dna Repair
- Dna Methylation
- Pathogenesis
- Cell Cycle
- Meiosis
- Dna Replication
- Localization
- Pachytene
- Prophase
- Cell Death
- Gene Silencing
PTMs
- Methylation
- Demethylation
- Phosphorylation
- Acetylation
- Cleavage
- Deamination
Research Areas
- Apoptosis
- Breast Cancer
- Cancer
- Cell Cycle and Replication
- Checkpoint signaling
- DNA Double Strand Break Repair
- DNA Repair
- Mismatch Repair
- Tumor Suppressors
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/MLH1
bosterbio.com/bosterbio-gene-info-cards/MLH1
References
PMID: 8145827 by Bronner C.E., et al. Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer.
PMID: 8128251 by Papadopoulos N., et al. Mutation of a mutL homolog in hereditary colon cancer.