LRTOMT Antibodies

Transmembrane O-methyltransferase (LRTOMT)

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones (By similarity). Required for auditory function (PubMed:18794526).

Component of the cochlear hair cell's mechanotransduction (MET) machinery. Involved in the meeting of the asymmetric tip-link MET complicated.

Gene Information

Human
Gene Name:	LRTOMT
Uniprot:	Q8WZ04
Entrez:	220074

Family

Belongs to:
class I-like SAM-binding methyltransferase superfamily

Alternative Names

leucine rich transmembrane and 0-methyltransferase domain containing; TOMT

Molecular Weight

Mass (kDA):
32.155 kDA

Genomic Context

Human
Location:	11q13.4
Sequence:	11; NC_000011.10 (72080342..72110782)

Subcellular Localizations

[Isoform 1]: Membrane; Single-pass membrane protein.; [Isoform 2]: Cytoplasm. Endoplasmic reticulum. Localized to the cell body of the cochlear hair cells, but is not present in the stereocilia (PubMed:28504928). Present but not restricted to the apical cistern, Hensen's body and the subsurface cistern (By similarity).

Diseases

• Complete Hearing Loss
• Deafness Congenital
• Sensorineural Hearing Loss (disorder)
• Deaf Mutism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LRTOMT

Best Uses Of The LRTOMT Marker

This is the place to go if your search for a new anti-body for your research is over. Boster Bio has developed a new antibody for the LRTOMT molecule that is specific and binds to the protein. Each product is also validated with positive and/or negative samples to ensure its high affinity and specificity. Product credits are awarded to the Boster team's first reviewers. The company rewards scientists from around the world for their early reviews.

LRTOMT Marker: Best Uses of the LRTOMTMarker

A boster bio LRTOMT antibody can be used to determine if a protein is a receptor. It reacts with Human, Mouse, and Rat. The antibody is stable if stored at 4degC for a month. It is made from trehalose and NaCl. You can also buy blocking peptides if you don't have access to boster-bio antibodies.

CCMCs, which are proteins that regulate potassium-dependent channels, such as Kv1.5 and Kv2.1, are proteins. Researchers created a CellELISA protocol that monitored the immune response to infected cells. This method showed that tumor cells had a high level of humoral responses against these transfected cells. The findings were published as Cell. The research team now plans to investigate LRTOMT more in depth.

The laboratory's main research theme is gene diversity and its role in disease. It aims to establish a multidisciplinary network of rare diseases in the region. It recommends that biobanks be established and that biostatistical skills and biotechnology are developed. Group studies on sequencing ADNs from North Africa population samples were also part of the symposium. They hope that others will follow their lead and study genetic diversity and causes of disease.

LRTOMT stands to mean leucinerich transmembrane or O-methyltransferase. The gene is located on 11q13.4 and evolved by merging two ancestral neighboring genes, Lrrc51 and Tomt. Lrrc51, Tomt, and Tomt are separate genes that encode distinct proteins in rodents. These two proteins can be encoded by either short or long transcript forms.

How to record the test results using autoradiography film

Autoradiography films use labels that emit chemiluminescent light to detect samples. Autoradiography films provide maximum clarity and signal-to-noise ratios. Autoradiography film is also equipped with a lightblue dye, which enhances the image's appearance if it is lit in a viewbox. The active component in autoradiography film is the emulsion. This is made of small silver halide crystals suspended with gelatin. Emulsions typically have a thickness of 10 um.

Two exposures are made to the tissue section. The first exposure is mostly "*/". The second exposure is mostly "C". The 18lI decay results in a darkened film. The second exposure takes several times as long as the first. The tests are labeled with mI and 14C. These labels are used to measure cerebral blood flow as well as deoxyglucose levels. The resulting images of the two exposures are used to record the test result.

How to validate IHC and WB antibodies

Validation of antibodies requires optimization of assay conditions including concentration, antigen retrieval buffer and primary antibody incubation conditions. To fully assess the specificity of an antibody's sensitivity and specificity, its dynamic range must be maximized. In some cases, antibodies raised against target antigens may not be able to bind to it. In these cases, it is necessary to use antibody-based assays even if antibodies were previously validated by Western blotting. Additionally, cross-reactive epitopes may be present in different samples, which can lead to varying patterns of nonspecific binding.

To avoid these potential pitfalls, ensure that your antibody has been approved for use in a variety of applications. WB, IHC and ELISA all have their place in validation of antibodies. While WB is considered the gold standard of immunohistochemistry, the process is time consuming and expensive for research laboratories. You should validate an antibody on multiple applications before you use it in a WB, IHC or immunohistochemistry analysis.

Other than WB, immunofluorescence or ICC, there are several other methods to validate your antibody. WB is a useful initial antibody screen. However immunoblot results may not represent a positive antibody during the final analysis. If a single band is detected at the expected molecular weight, it is considered a positive result. Multiple bands indicate a negative reaction.

Standardizing antibody validation is a collaborative effort by the vendor, researcher, as well as the publisher. In addition to validating antibody performance, the user must design well-designed experiments. Vendors should provide high-quality antibodies as well as detailed disclosure of their methods. Publishers can help create and enforce guidelines for antibody validity validation. Working together, we can develop uniform standards and improve antibody quality in the biomedical world.

An effective antibody validation method is genetic manipulation of the target protein. This allows researchers to link the genetic base of a target protein with its protein product and provides evidence that the antibody binds to the target. There are several methods to manipulate the expression of the target protein. CRISPR/Cas9 technology, which uses genetic manipulation of cell lines to create custom single gene knockouts, is an example of such a technique.

A Western blot with the primary and second antibodies is done separately to verify the specificity. Cross-reactive bands must be avoided during the test. This will compromise data interpretation. Before you proceed with the IHC assay, make sure to verify the specificity. However, in this case, RNA-seq is the most elegant validation method.