DNA ligase 3 Antibodies

DNA ligase 3 (LIG3)

Isoform 3 acts as heterodimer with DNA-repair protein XRCC1 in the nucleus and may fix defective DNA strand- break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it acts as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627).

.

Gene Information

Human
Gene Name:	LIG3
Uniprot:	P49916
Entrez:	3980

Family

Belongs to:
ATP-dependent DNA ligase family

Alternative Names

DNA ligase 3; DNA ligase III; EC 6.5.1.1; LIG2; ligase II, DNA, ATP-dependent; ligase III, DNA, ATP-dependent; Polydeoxyribonucleotide synthase [ATP] 3

Molecular Weight

Mass (kDA):
112.907 kDA

Genomic Context

Human
Location:	17q12
Sequence:	17; NC_000017.11 (34980498..35009743)

Tissue Specificity

Testis, thymus, prostate and heart.

Subcellular Localizations

[Isoform 1]: Mitochondrion. Contains an N-terminal mitochondrial transit peptide.; [Isoform 2]: Mitochondrion. Contains an N-terminal mitochondrial transit peptide.; [Isoform 3]: Nucleus. Lacks the N-terminal mitochondrial transit peptide.; [Isoform 4]: Nucleus. Lacks the N-terminal mitochondrial transit peptide.

Diseases

• Malignant Neoplasms
• Carcinoma
• Neoplasms
• Malignant Neoplasm Of Breast
• Malignant Neoplasm Of Urinary Bladder
• Mammary Neoplasms
• Bladder Neoplasm
• Radiation Injury
• Adenocarcinoma
• Genomic Instability
• Diabetes Mellitus
• Cancer Of Nasopharynx
• Chromosomal Translocation

• Carcinoma, Transitional Cell
• Tumor Progression
• Malignant Neoplasm Of Lung
• Carcinogenesis
• Nervousness
• Lung Neoplasms
• Tissue Adhesions

Interacting Proteins

• MAX

Pathways

• Dna Repair
• Dna Replication
• Cell Cycle
• Cell Adhesion
• Base-excision Repair
• Dna Alkylation
• Developmental Process
• Cellular Homeostasis
• M Phase
• V(d)j Recombination
• Spermatogenesis
• Double-strand Break Repair
• Cytokinesis

• Cell Division
• Female Meiosis
• Cell Differentiation
• Chromatin Modification
• Meiosis
• Gastrulation
• Dna Methylation

PTMs

• Phosphorylation

• Dephosphorylation

Research Areas

• Base Excision Repair
• Cancer
• DNA Repair

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LIG3

Boster Bio: Best Uses Of The LIG3 Marker

This article will cover the many benefits and costs of the Boster Bio LIG3Marker. It also explains some of its most common uses. Continue reading to learn more about this marker and make an informed decision about its suitability for your research. Here are some examples of the benefits and common uses of this marker. You can also learn more about the application options, and answer common questions about this marker. Let's get started!

The LIG3 Marker has many benefits

The LIG3 marker is a genetic marker that detects the presence of the gene LIG3. It is a protein essential for the replication and maintenance of DNA outside of Mitochondria. It is important in the diagnosis and treatment of cancer. Its presence in cells can be used to help determine the treatment options for patients with Leukemia. LIG3 can be found in all human tissues.

Common uses

The LIG3 gene has been used in various applications in biology. The gene can be found in many branches and ancestors of the metazoan tree. However, it is rare in model organisms. Lig3 homologs can also be found in slime mold and fruit flies. Lig1 and Lig3 share 60% of their sequence identity. However, there is only 20% sequence identity between Lig3 and Lig4.

The 1960s saw the first isolation of LIG3 and it has since been widely used as a molecular genetic tool. It is found within all three major life forms. Lig3 is found in almost all cells in the human genome as well in most of the ancestral eukaryotes. However, it has been questioned as to whether it is located in mitochondria. The LIG3 genes are expressed in multiple cell types. Therefore, the exact function of this gene is not known.

The LIG3 gene encodes many DNA ligases, including nuclear LIG3 as well as mitochondrial LIG3. They are ATP-dependent. They seal breaks in duplex genetic material. They are an essential component of the excision process. However, LIG3 is also associated with mitochondrial DNA metabolic. It also contains a specialized Nterminal ZnF. This plays a critical role for sensing and intermolecularly ligating DNA molecules.

Cost

LIG3 expression in KD cells is 90% reduced. The LIG3 gene marker is not associated with mtDNA copy, but it is believed that 10% of its levels are sufficient for HeLa cells to maintain a normal mtDNA copy. The LIG3 marker is not cheap, despite its low price. So how much does the LIG3 markers cost?