LAMP3 Antibodies

Lysosome-associated membrane glycoprotein 3 (LAMP3)

May play a role in dendritic cell function and in adaptive immunity. .

Gene Information

Human
Gene Name:	LAMP3
Uniprot:	Q9UQV4
Entrez:	27074

Family

Belongs to:
LAMP family

Alternative Names

CD208 antigen; CD208; DCLAMP; DC-LAMP; DCLAMPDC-lysosome-associated membrane glycoprotein; LAMP; LAMP3; LAMP-3; lysosomal-associated membrane protein 3DC LAMP; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403; TSC403CD208

Molecular Weight

Mass (kDA):
44.346 kDA

Genomic Context

Human
Location:	3q27.1
Sequence:	3; NC_000003.12 (183122215..183162761, complement)

Tissue Specificity

Detected in tonsil interdigitating dendritic cells, in spleen, lymph node, Peyer's patches in the small instestine, in thymus medulla and in B-cells (at protein level). Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.

Subcellular Localizations

Lysosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3 (PubMed:9768752). Detected on lysosomes in mature dendritic cells (PubMed:9768752).

Diseases

• Malignant Neoplasms
• Neoplasms
• Neoplasm Metastasis
• Parkinson Disease
• Mammary Neoplasms
• Adenocarcinoma
• Malignant Neoplasm Of Breast
• Lung Neoplasms
• Pulmonary Fibrosis
• Autoimmunity
• Hypoxia
• Lung Diseases
• Malignant Tumor Of Cervix

• Carcinoma
• Psoriasis
• Uterine Cervical Neoplasm
• Cell Invasion
• Malignant Squamous Cell Neoplasm
• Lymphatic Metastasis
• Albinism

Pathways

• Localization
• Transport
• Pathogenesis
• Secretion
• Translation
• Cell Cycle
• Cytokine Production
• Cell Adhesion
• Aging
• Immune Response
• Platelet Activation
• Transcytosis
• Tropism

• Platelet Aggregation
• Mrna Stabilization
• Cell Differentiation
• Exocytosis
• Dna Methylation
• Glycosylation
• Synaptic Transmission

PTMs

• Phosphorylation
• Cleavage

• Glycosylation
• Methylation

• Deglycosylation

Research Areas

• Dendritic Cell Markers

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/LAMP3

Best Uses of the LAMP3 Marker in Boster Bio

Among the best uses of the LAMP3 marker is to detect the expression of specific genes, such as LAMP3 (leucine-macropeptide 3). The LAMP3 gene encodes a polypeptide that is highly expressed in many organisms. Boster Bio is able to facilitate its production. This gene is found in many species, including humans. This gene, which is found in the boster, can be of benefit to scientists all over the world.

Anti-DC-LAMP LAMP3 Marker

Boster Bio's Anti-DC-LAMP LAMP3 Marker is a ligand to the UNC5B and DCC1 receptors. Both molecules can have an impact on the activity of p53, and could be involved in tumor invasion. Both DCLAMP (and Sox9) are lysosomal membrane related proteins. These proteins are essential in dendritic cell proliferation and in tumor invasion. These markers are also associated with cell proliferation and tumor formation.

The DC–LAMP antibody is a member of the LAMP protein family. It is highly glycosylated and contains 496 amino acids. It is expressed by mature dendritic cells within T-cell areas, also known as interdigitating dendritic cells. Its expression has also been linked to CD86 expression. Therefore, the Anti-DC-LAMP LAMP3 marker in Boster Bio is an excellent tool for immunodiagnostic studies.

Storage

Different cell types express the LAMP3 marker. The mechanism that regulates its expression has been studied. The expression of fluorescent-tagged proteins can detect LAMP3 in different cell types. Real-time PCR was also used to measure LAMP3's mRNA levels. This study highlights the important role of LAMP3 in Salmonella induction. Its expression is dependent not only on the presence of Salmonella but also on its proximity to the pathogen.

Several studies have linked LAMP3 expression to autophagy and drug sensitivity. However, the process by which Salmonella infects humans is not as well understood and studied as other LAMP proteins. Because of this, researchers have studied LAMP3 to determine if it plays a role in the pathogenesis of Salmonella infection. Although the exact role of this gene in cancer development is unknown, it has been shown to be associated with several types of cancer.

The LAMP3 genes can be found in many organs including the liver. Autophagy is significantly influenced by LAMP3 transcription. Its suppression results in an inhibition of autophagy and ultimately, apoptosis. LAMP3 is also involved with autophagic death. Cathepsin-mediated permeation is believed to be the primary mechanism for lysosomal-associated cell death.

The LAMP3 genome belongs to the LAMP3 family. High glycosylation is characteristic for LAMP3. It is associated with cancer metastasis. It has been identified as a molecular marker in mature dendritic tissues. However, epithelial expression of the gene has not yet been studied in esophageal Squamous Cell Carcinoma. The gene was detected using immunohistochemistry. To check for possible correlations with gene expression, the DNA copy number was also examined.

Usage

LAMP3 is one of the lysosomal glycoproteins. It is an integral membrane proteins that is expressed on cellular surface. It was first discovered in human adendritic cells during their maturation. It transiently interacts with MHC class I molecules at the limit membrane of intracellular spaces. LAMP3 was independenty characterized as a lung-specific gene. The gene was then designated TSC403 transcript.

LAMP3 is highly expressed in human tumors. LAMP3 expression is high in primary cancers. It may play a role both in tumor metastasis as well as resistance to certain treatments. It could be the next molecular sign of cancer. Many primary tumors express elevated LAMP3 expression. LAMP3 levels are associated with hypoxia regulation. Epithelial LAMP3 levels have been identified as a prognostic biomarker of esophageal carcinomas. In addition, it has been associated with several tumor metastases.

LAMP3 can be expressed in many cell types. Researchers have looked at how it is inducible in various cell types. They also studied mechanisms that regulate LAMP3 induction. It is not a good idea just to rely on LAMP3, but it is a useful marker to study the cell's quality control. For more information, please see the paper published in Experimental Biology 2019 Meeting.

Type II pneumocytes are high in LAMP3. These cells are responsible for multiple functions including surfactant synthesis and secretion as well as recycling. It is also found on human DCs. It is also found in the lysosomes, but not on the plasma membrane.

Applications

The LAMP3 marker may have potential applications in cardiac remodeling. It is a member or the transmembrane 4 Superfamily, also known under the tetraspanin name. This superfamily includes four hydrophobic domains which control cell activation as well as development. One function of this protein is to interact with Integrins, which are proteins that regulate cell adhesion. There are many reports that the LAMP3 gene is associated with different types of tumors.

Mutations in this gene can lead to similar clinical symptoms and pathology in animal and human models of lung disease. Airedale Terrier dogs, which have a severe neonatal sifactant deficiency, are an example. The gene is critical in lung biology and surfactant biology. LAMP3 plays a crucial role in lung physiology and surfactant homeostasis.

This study used CRISPR/Cas9 genetic editing technology to create null Lamp3-producing mice. The CRISPR system allowed us to combine Cas9 with two single-guide RNAs (sgRNAs), that target the Lamp3 exon. We injected Lamp3-/ mice into fertilized zygotes. The resulting founder mice had normal lung function and no increase of prenatal mortality. PCR amplification of BAL fluid from the Lamp3-/ mice showed an increase in pro-SP-C and an altered lipid composition.

LAMP3 can be used to detect tumors. It aids in the detection and treatment of tumors with high levels of this gene. Its overexpression has been linked with increased invasion and migration to lymph-vascular spaces. These discrepancies need to be clarified. The LAMP3 marker, however, is a promising new tool to evaluate tumor progression and prognosis. Its popularity is rising rapidly.