KIF20B Antibodies

Kinesin-like protein KIF20B (KIF20B)

Plus-end-directed motor enzyme that's required for completion of cytokinesis (PubMed:11470801, PubMed:12740395). Required for proper midbody organization and abscission in polarized cortical stem cells.

Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons at a tubulin and actin-dependent method.

Gene Information

Human
Gene Name:	KIF20B
Uniprot:	Q96Q89
Entrez:	9585

Family

Belongs to:
TRAFAC class myosin-kinesin ATPase superfamily

Alternative Names

Cancer/testis antigen 90; CT90MPP-1; DKFZp434B0435; EC 6.3.5.5; kinesin family member 20B; Kinesin-related motor interacting with PIN1; KRMP1kinesin-like protein KIF20B; M-phase phosphoprotein 1mitotic kinesin-like protein; MPHOSPH1EC 2.1.1; MPP1DKFZp434P0810

Molecular Weight

Mass (kDA):
210.629 kDA

Genomic Context

Human
Location:	10q23.31
Sequence:	10; NC_000010.11 (89701590..89774943)

Tissue Specificity

Brain, ovary, kidney and testis (at protein level) (PubMed:12740395). Overexpressed in bladder cancer cells (at protein level) (PubMed:17409436). Expressed in testis. Overexpressed in bladder cancer cells (PubMed:17409436).

Subcellular Localizations

Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Midbody. Cell projection, axon. Cell projection, growth cone. Localizes mainly in the nucleus during interphase although it is also detected in the cytoplasm without clear association with microtubules (PubMed:12740395). Localized to the central spindle during cytokinetic furrowing and with the midbody during abscission (PubMed:12740395, PubMed:17409436). A 2-3 fold expression increase is seen as cells pr

Diseases

• Malignant Neoplasms
• Neoplasms
• Ataxia
• Malignant Neoplasm Of Urinary Bladder
• Adenocarcinoma
• Bladder Neoplasm
• Sclerosis
• Adenoma
• Primary Lateral Sclerosis
• Polyneuropathy
• Malignant Neoplasm Of Breast
• Glioma

• Hypoxia
• Amyotrophic Lateral Sclerosis
• Nodular Goiter
• Infertility
• Carcinoma
• Paroxysmal Nocturnal Hemoglobinuria
• Goiter

Pathways

• Mitosis
• Interphase
• Cell Death
• M Phase
• Cytokinesis
• Cell Cycle
• Pathogenesis
• Centromere Separation
• Programmed Cell Death
• Induction Of Apoptosis
• Fertilization
• Metaphase
• Insemination

• Cell Division
• Translation
• Prophase
• Cell Growth
• Mitotic Recombination
• Microtubule Polymerization
• Rna Interference

PTMs

• Phosphorylation

• Reduction

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KIF20B

Best Uses Of The KIF20B Marker

The Boster Bio KIF20B is a molecular marker with high specificity and potency that targets a protein. This article will cover the clinical applications of the marker as well as the indications. In addition, we will discuss the pipeline for generating a molecular signature. These are just a few of the many benefits of this marker.

Indications

The maturation and maturation of middlebodies is affected by the KIF20B gene marker. The midbody comprises a set of proteins that act as middle bodies and prepare them for abscission. The complex midbody region is characterised by a series of events that includes the recruitment of proteins to bulges and formation of rings, thinnening of flanks, shedding membrane, and establishment constriction sites. These processes may be coordinated by KIF20B.

During early anaphase the KIF20B microtubule marker associates with central spindle Microtubules. The marker accumulates at microtubule Plus ends. During abscission, KIF20B remains concentrated at constriction sites. KIF20B loss is associated with abscission delays. However, the markers do not have to be present in order to encourage the formation constriction sites.

The average area in midbodies of the CEP55 sign after siLUC removal is similar to that in cells treated using siKIF. However, siKIF-treated cell have a significantly longer median time to disintegrate. The median time for abscission in siKIF cells was significantly longer when compared to siLUC treated cells.

KIF20B is enriched for the mTOR signaling pathway and JAK-STAT pathway. KIF21B mediates immune suppression by being involved in the T cells receptor signaling pathway. It may therefore be a promising target for immunological research. However, more research is needed before KIF20B can be considered a useful tool in the diagnosis and treatment PDAC.

KIF20B markers are not specific genes for cancer patients. It is associated with cancer progression. Its expression correlates well with the size and shape of the tumor. The correlation coefficient is positively and the p value is 0.68. Similar to the above, downregulation of KIF20B results in the arrest of the mitotic process in gastric carcinoma cells. It is also implicated in breast and pancreatic cancer.

While KIF20B has been associated with cancer, it was initially identified as a plus-end directed kinesin-associated protein. It is phosphorylated at the G2/M transition and has the ability to influence microtubule bundles. It is necessary for cytokinesis. Nevertheless, it is not yet understood exactly how KIF20B regulates cell cycle progression.

While KIF20B is a potential biomarker for cancer, it has yet to be fully understood. In addition to its association with cancer progression, it is also associated with poor patient survival. This gene is involved with cell proliferation and targets the p53 protein. Further research is needed to determine its role in cell proliferation and its mechanisms. KIF20B, a candidate cancer-testis biomarker for oral squamous carcinoma, remains an option.

The KIF20B gene was previously associated with poor prognosis in tongue cancer. It is believed to play a significant role in tumor development. KIF20B expression in tongue cancer patients was linked with lymph node metastasis, tumor stage, and gene expression. It has been associated with poor prognosis for many types of cancer. Poor prognosis in hepatocellular and other cancers has been linked to KIF20B.

Clinical applications

Researchers recently reported that KIF20B may play a part in cancer. This gene promotes cell proliferation in vitro and high levels have been associated with poor prognosis in bladder and breast cancer. The gene could be knocked down to reduce cell proliferation in pancreatic carcinoma cell lines. This marker could help doctors detect this type of cancer early. However, clinical applications of this gene are still limited.

Several autoantigens have been identified. For example, CENP-F is a high-molecular-weight protein that has similar IIF staining patterns. NuMA, a coiled coil protein located at th mitotic spindle pole, is another autoantigen. This group also includes Nonmusclemyosin (cytoskeletal protein). KIF20B's extensive coiled coil region results in a higher sensitivity to serum samples from humans.

By immunoprecipitation, the KIF20B gene first was isolated from keyhole limpet cell cells. Immunoglobulin G (IgG) antibodies were raised against the peptide. These antibodies showed high immunoreactivity to purified KIF20B. 10C7 antibody outperformed index human anti-KIF20B five-fold. These results suggest the KIF20B gene is an important marker in the diagnosis of many diseases and conditions.

The KIF20B gene is a member of the kinesin-6 family. The protein is a critical regulator of cell-cycle cytokinesis. A contractile ring drives ingression of the cleavage furrow. The midbody anchors abscission machinery and acts as a platform for the cleavagefurrow. KIF20B (Kirsten Inferiority) is required for effective midbody maturation.

The KIF20B genes plays an important part in cytokinesis, and is crucial for cell division. KIF20B is knocked down, which results in the accumulation of multinucleated cell that eventually undergo apoptosis. It is important to remember that the gene regulates by two proteins: FBXO38 (USP7). These proteins can be inhibited to significantly increase the number of multinucleated cellular cells in knockdown studies. This indicates that they play a key role in KIF20B's cytokinesis.

Previously, researchers have found that silencing the USP7 protein reduces KIF20B levels in midbodies. Although the effect was modest, it was still significant. These results suggest that silencing KIF20B may affect the timing of abscission. This new marker could play a role during cytokinesis. This novel gene will be useful for many types of cancer patients.

It was found that the KIF20B gene is overexpressed in cancer cells. KIF20B expression was increased in cells treated using siKIF, despite Skp1 being absent. KIF20B was expressed more in cells treated by siKIF than cells without Skp1. This suggests KIF20B could play an important part in central spindle organization. It may also promote furrow expansion. It can also facilitate cell division in cancer.

Molecular signature generation pipeline

A biological(bio)-molecular inspired electronic architecture (BIA) defines nanoscale sensor platforms. Biological elements are used to establish high-level functions and enable communication within nanoelectronic devices. BIA can also establish hybrid Electro-THzOptical (ETO), communication channels. The bio-molecules embedded in the architecture are directly related to THz-frequency characteristics, which allow enhanced sensing THz biosignatures.