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- Table of Contents
Facts about ATP-sensitive inward rectifier potassium channel 1.
Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Human | |
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Gene Name: | KCNJ1 |
Uniprot: | P48048 |
Entrez: | 3758 |
Belongs to: |
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inward rectifier-type potassium channel (TC 1.A.2.1) family |
ATP-regulated potassium channel ROM-K; ATP-sensitive inward rectifier potassium channel 1; inwardly rectifying K+ channel; KIR1.1; potassium inwardly-rectifying channel, subfamily J, member 1; ROMK1inwardly rectifying subfamily J member 1
Mass (kDA):
44.795 kDA
Human | |
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Location: | 11q24.3 |
Sequence: | 11; NC_000011.10 (128838020..128867296, complement) |
In the kidney and pancreatic islets. Lower levels in skeletal muscle, pancreas, spleen, brain, heart and liver.
Cell membrane; Multi-pass membrane protein. Phosphorylation at Ser-44 by SGK1 is necessary for its expression at the cell membrane.
Boster offers high affinity primary antibodies that have been extensively validated in ELISA, Immunohistochemistry, and Western Blotting. Boster antibodies perform as well as the original manufacturers promise. Boster antibodies were also cited over the last 25 years. Therefore, you can trust Boster products. Continue reading to learn more about Boster Bio and KCNJ1 Marker.
KCNJ1 is a gene that encodes a protein which allows potassium to flow into cells. It is found in kidneys and is associated to Bartter syndrome, an autosomal recessive disease that causes hypercalciuria, hypokalaemia and hyporenininemic hypoaldosteronism. Boster Bio developed antibodies using mouse and rabbit to combat this gene.
Voltage-gated sodium channel (VGSCs), regulate the influx Na+ into excitable cell and mediate its proliferation, producing an action potential. VGSCs can also be found in non-excitable cell types and play a variety of biological roles. They are composed pore-forming subunits. Nine genes make up the Nav1 subunits. Each subunit is composed of four homologous domains as well as six transmembrane segments.
A hybrid model that combines both the ligand/receptor model with the mechanical lever model is the basis of the mechanism for NaV channels. Both models predict that S4 and S5L peptides will bind to S6T, leading to a high-affinity open condition. The S4 - S5L peptides have amino-acids that are key to stabilizing the channel in the open state.
The a subunit is made up of four repeat domains as well as six membrane-spanning segments. The voltage sensor function is performed by the highly conserved S4 segments. Positive amino acids are found at every third position in the pore. As the positive amino acid move towards the extracellular membrane, they create a pore which conducts ions. The pore is divided into two regions. The P-loops are on the more external side.
Normally, the axonal membrane has a resting potential around -70mV in most neurons. Negative ions, which are not allowed to enter the pore, are prevented from entering by activation gates. On the other hand, positive ions flow into the narrowed portion of the pore. This results in an increase in voltage across the neuronal membrane.
Metastatic cell behavior may be affected by voltage-gated Na+ channels activity. Functional VGSC activity can influence invasion and endocytosis. In vitro experiments showed that tetrodotoxin (10 mmol/L), significantly inhibits the activity of VGSCs. The cells' proliferation was not affected by a higher concentration of tetrodotoxin. Additionally, transwell migration was not affected even though the tetrodotoxin concentrations were lower.
Future studies should investigate the role played by ion channels within the regulation of Warburg's effect. This will make the Warburg phenomenon more clinically applicable. This will enable clinical application of the evidence. It is possible that pharmacological interventions targeting tumor development and cancer metabolism remodeling might prove to be effective. The findings of this study are encouraging. This knowledge has the potential to be applied in a major way. The discovery of a drug to target Nav1.5 could have a significant effect on cancer treatments.
In the case of eukaryotic NaV channel, however, the peptide S4–S5L–peptide strengthens the binding between S6T and S4–S5L–H. These channels may be enhanced by the interaction of the disulfide-bridge between the two subunits. In fact, if the peptide S4-S5L-peptide enhances S6T-mediated channel opening, it can be the basis for the development of a new class of NaV-based drugs.
Boster Bio stocks antibodies to the potassium channel protein KCNJ1. These antibodies have been tested and validated for use in ELISA, Immunohistochemistry, and WB. These antibodies react to human, mouse, or rat cell lines. They contain Trehalose. Boster Bio has the right antibody for you, whether you need potassium channel antibodies for your protein of choice.
PMID: 7929082 by Shuck M.E., et al. Cloning and characterization of multiple forms of the human kidney ROM-K potassium channel.
PMID: 8190102 by Yano H., et al. Alternative splicing of human inwardly rectifying K+ channel ROMK1 mRNA.