JAML Antibodies

Junctional adhesion molecule-like (JAML)

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair.

Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.

Gene Information

Human
Gene Name:	JAML
Uniprot:	Q86YT9
Entrez:	120425

Family

Belongs to:
immunoglobulin superfamily

Alternative Names

adhesion molecule, interacts with CXADR antigen 1; AMICA; AMICA1; CREA7-1; Gm638; JAML

Molecular Weight

Mass (kDA):
44.339 kDA

Genomic Context

Human
Location:	11q23.3
Sequence:	11; NC_000011.10 (118193725..118225089, complement)

Tissue Specificity

Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes (at protein level).

Subcellular Localizations

Cell membrane; Single-pass type I membrane protein. Cell junction. Localized at the plasma membrane and enriched in areas of cell-cell contacts (PubMed:12869515).

Diseases

• Leukemia
• Tissue Adhesions
• Myeloid Leukemia
• Neoplasms
• Acute Promyelocytic Leukemia
• L1 Acute Lymphoblastic Leukemia

• Acute Lymphocytic Leukemia

Pathways

• Cell Adhesion
• Methylation
• Integrin Activation
• Dna Methylation
• T Cell Costimulation
• Cell Recognition

• Localization

PTMs

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/JAML

Best Uses Of The JAML Marker From Steven Boster

Steven Boster products are a great option for those who have been reluctant to use high-affinity primary antibodies. Boster's antibodies are highly cited and have been thoroughly validated for use in Western Blotting, Immunohistochemistry, and ELISA. Here's why. Continue reading to learn more about Steven Boster’s JAML marker.

JAML Marker

JAM Oil Based Premium Paint Marker is a permanent ink that dries quickly and can be used on any surface. Its pigment is waterproof and fade-resistant. It can be used to decorate windows and walls, as well creating beautiful and colorful drawings. This marker is also resistant towards abrasions and corrosion, so you can use it on many surfaces.

The JAML ligand, a functional protein found in the epidermis is able to bind to soluble adenovirus fibre protein. This protein can also bind to keratinocytes and its soluble form binds on the cell's surface. In this study, soluble JAML only bound to keratinocytes and binding to F5 displaced or blocked JAML.

Primary antibodies of high-affinity

The JAML marker can be used to detect a specific antigen within a tissue section. High-affinity prima antibodies can be used for immunohistochemistry as well as biomarker research. These antibodies are made to bind to a specific antigen and have high specificity and affinity. Primary antibodies are produced using animals as hosts. JAML is the acronym for joanneaml antigen membrane ligand.

Monoclonal bD1 monoclonal antibody is the basis of high-affinity primaries. This monoclonal antibody can be expressed in HEK cells as a full-length, human IgG. Although the bD1 was not originally mentioned in this paper, it has been shown that it has a greater HER2 binding power than the monoclonal antibody. The authors also discovered that the bD1 antibody contains a cysteine at the 100c position, which might interfere with the binding of the heavy chain to HER2.

Flowcytometry is not well-suited for antigen concentration determination, as mammalian cell cultures are often heterogeneous. Flowcytometry is often used to identify several variants of antibodies in engineering projects. Each variant shows a slight increase in affinity relative the clone that preceded it. The Fab structure of the full length IgGs is preserved by the Fab scaffold. This avoids the bivalent nature that the protein has. The JAML markers reduce avidity effects.

Boster offers a range of high-affinity primary antibody against a variety o antigens. Most of these antibodies are recommended by the research world after they have been rigorously validated in multiple research projects. Many of these antibodies have been tested against knockouts and for protein arrays. For the JAML marker, Boster has also validated its antibodies using knockouts and phage display. There are many benefits to using primary antibodies.

The JAML marker is a good candidate for identifying HER2-Fc-expressing cells. The JAML markers can be used in vitro. The human antibodies have been purified using CHO–T cells. This kit allows scientists to test potential antibodies' RNA binding properties. These antibodies are available at R&D Systems.

The binding to JAML was also tested on the purified antibody variants. The non-reduced bD1 (and hu4D5) were both tested for binding to JAML. This antibody binds to a light chain with an overlapping band near the elution volume. The light chain of bD1 antibodies is glycosylated. However, the non-glycosylated bands migrate at the highest volume.

These two companies also use animal vaccination as a method to identify antibody candidates. During the immunization process B-cells can be harvested and hybridomas made. The hybridomas are screened for antibodies that can replicate the original antigen-binding capability. In vitro polarization antigen-binding-B-cells can be achieved, which results in smaller mammalian display collections with high affinity and functionalities.