IGSF8 Antibodies

Immunoglobulin superfamily member 8 (IGSF8)

May play an integral role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus operate. May regulate proliferation and differentiation of keratinocytes.

May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to curb prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as crucial linkers. May also play a role on integrin- dependent morphology and motility functions.

Gene Information

Human
Gene Name:	IGSF8
Uniprot:	Q969P0
Entrez:	93185

Family

Belongs to:
No superfamily

Alternative Names

CD316 antigen; CD316; CD81P3; CD81P3LIR-D1; EWI2; EWI-2; EWI2CD81 partner 3; Glu-Trp-Ile EWI motif-containing protein 2; IGSF8; immunoglobulin superfamily, member 8; KCT4; KCT-4; Keratinocytes-associated transmembrane protein 4; PGRL; PGRLimmunoglobulin superfamily member 8

Molecular Weight

Mass (kDA):
65.034 kDA

Genomic Context

Human
Location:	1q23.2
Sequence:	1; NC_000001.11 (160091339..160099442, complement)

Tissue Specificity

Expressed in brain, kidney, testis, liver and placenta with moderate expression in all other tissues. Detected on a majority of B-cells, T-cells, and natural killer cells but not on monocytes, polynuclear cells and platelets.

Subcellular Localizations

Cell membrane; Single-pass membrane protein.

Diseases

• Neoplasms
• Tissue Adhesions
• Nervousness
• Cell Invasion
• Hepatitis
• Hepatitis C
• Carcinoma
• Malignant Neoplasms
• Malaria
• Malignant Paraganglionic Neoplasm
• Virus Diseases
• Neoplasm Metastasis

• Epithelioma
• Tumor Progression
• Leukemia, T-cell
• Glioma
• Hypoxia
• Malignant Neoplasm Of Liver
• Infertility

Interacting Proteins

• KRTAP10-4
• KRTAP10-7
• KRTAP10-8
• KRTAP10-9

Pathways

• Cell Migration
• Localization
• Cell Adhesion
• Cell Motility
• Cell Activation
• Cell Proliferation
• Protein Palmitoylation
• Entry Into Host
• Fertilization
• T Cell Activation

• Regeneration
• Wound Healing
• Cell Recognition
• Cell-cell Adhesion
• Secretion

PTMs

• Palmitoylation
• Phosphorylation

• Depalmitoylation
• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/IGSF8

Best Uses of the IGSF8 Marker

The IGSF8 indicator is a powerful tool in cancer diagnosis. Its use in tumor research is increasing rapidly. It has been found in tumor stem cells, astrocytes, oligodendrocytes, and a variety of other cells. Learn more about this unique protein. It can react to multiple species. Find out its best uses. This article will give you an overview of the IGSF8 Marker as well as its many uses.

IGSF8 Marker

IGSF8, a member of EWI's subfamily of proteins, has a distinct Glu-Trp/Ile extracellular pattern. It interacts extensively with many other proteins, but is associated with CD81 and CD82 tetraspanins. These proteins are small extracellular domains that are not cell-surface receptors, but are involved in organizing proteins within membranes.

OSNs express IgSF8 via their axon terminations. OSNs express IgSF8 through terminals and distal Axons. These findings suggest that IgSF8 plays an important role in developing the olfactory sense. However, further studies are needed to confirm the importance of this marker in olfactory development.

IGSF8 Marker in Tumor Stem Cells

The IGSF8Marker is a transcription element that regulates several biological functions. Tumor stem cells that lack Igsf8 in leukemia have a higher number of pro-apoptosis genetics and a lower number of anti-apoptosis genetics. They also express less b-catenin. Therefore, the IGSF8 Marker in tumor stem cells may contribute to cancer progression and resistance.

The CD133 marker is a promising candidate for identifying tumor stem cells in brain cancer. Although its biological significance is not known, this marker has been shown to be associated with glioblastoma. Its identification will prove to be a powerful tool in the investigation of tumorigenesis and development of new treatments. This marker could be used in chemotherapy to target cancer stem cell cells.

IgSF8 is a member the Immunoglobulin family and interacts with the immune system. Its primary function is to inhibit T-cell movement and immunosuppress. It also interacts avec the integrin apha-3/beta-1. It has been linked with poor prognosis for cancer and other types. It is unclear what exact role IgSF8 has in cancer.

It is now possible that a number markers can be identified as being related to cancer stem cell. Cancer stem cells express SHH (Bmi-1), Oct4, PSP (and Snail) These markers are involved in cancer stem cell maintenance. Cancer cells are often dysregulated in the JAK-STAT signaling pathway, which suggests a role in stem cell maintenance. In addition, JAK/STAT is required for stem cell self-renewal and niche anchoring.

The human IgSF8 full gene was subcloned in an expression vector, pLTC. It contains a CMV promor and a select marker. The vectors can be expressed either transiently in mammalian cells or permanently. A lentivirus vector that contains multiple antibiotic selection markers is now available for research. However, it is not recommended for human patients. This study found that OGT knockdown led to decreased tumor cell growth and an increase in CCSC number.

CD44, another CSC marker, has also been identified. This protein is highly expressed in certain types of hematological tumors. It is also an effective therapeutic target in non-small-cell lung carcinoma. More research is needed to determine whether CD44 is a viable target for cancer. CD44 is an important marker for non-small cell lung and ovarian cancers.

IGSF8 Marker in tumor astrocytes

Numerous studies have shown astrocytes are important in the progression of various types and types of cancer, such as gliomas. Some of these studies indicate that astrocytes may respond differently to oncogenic mutations, particularly if the tumor is large or is composed of multiple subpopulations. These tumors can also be heterogeneous depending on the cell origin.

Using a combination of mouse and human astrocytoma tissue, researchers detected the IGSF8 Marker, also known as EWI-2, in several tumor cell lines. However, tumors expressing EWI-2 were mostly negative. EWI-2-expressing tumors had a significantly lower chance of growing than normal astrocytes. Therefore, it is possible that the astrocytoma may be inhibited in vivo by EWI-2. However, this observation may not be fully conclusive until more studies are conducted.

One study found that GFAP astrocytes grew at a different rate than GLAST astrocytes. While GLAST astrocytes grew at a faster rate than GFAP astrocytes, the rate was slower in GS compared to OFB. This study also showed regional differences of proliferation rates between these two astrocyte types. It is important for you to know that tumor gliomas could differ in the expression of these markers as well as their tumor burden.

Interestingly, tdTomato astrocytes showed higher levels of GLAST expression in tdTomato than GFAP astrocytes. This implies that GLAST specifically targets these cells. Furthermore, tdTomato animals had higher targeting efficiency than NeuN+ neuron mice, suggesting that GLAST targets better astrocytes than it does gliomas. This study also suggests that GLAST may be an important target in the treatment for gliomas.

IGSF8 Marker in tumor oligodendrocytes

The expression of IGSF8 Marker is a sign of a cellular source for oligodendroglioma. This cell type is closely related to oligodendrocytes, a subtype of glial cell tumors. Oligodendroglial cancers are formed from precursor cells of oligodendrocytes. This cell lineage can differentiate tumors from those that aren't. To be successful in diagnosing tumor glioma cells, IGSF8 is a critical step.

Igsf8 is a transcription factor that is expressed by leukemia stem cells. It regulates Wnt/b/catenin signals and increases expression of pro-apoptosis proteins. It also modulates cell adhesion. This marker is associated in prognosis but its role is not known for a variety of cancers.

The oligodendrocytes of rodents are able to express the OLIG gene by using single-molecule multiplex mRNA hybridization to detect IGSF8 from tumor oligodendrocytes. OLIG cells work in concert with other cell types in order to express the oligodendrocyte marker OLIG1.

OLIG gene, in addition to being expressed by human oligodendrocytes is also highly expressed in glial cells tumors. GFAP expression was absent in oligodendrogliomas that expressed high levels of OLIG. Moreover, both OLIG expression and GFAP expression was absent in oligodendrogliomas.