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- Table of Contents
Facts about Homeobox protein Hox-B9.
Human | |
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Gene Name: | HOXB9 |
Uniprot: | P17482 |
Entrez: | 3219 |
Belongs to: |
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Abd-B homeobox family |
homeo box 2E; homeo box B9; homeobox B9; Homeobox protein Hox-2.5; Homeobox protein Hox-2E; homeobox protein Hox-B9; HOX-2.5; HOX2EHOX2
Mass (kDA):
28.059 kDA
Human | |
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Location: | 17q21.32 |
Sequence: | 17; NC_000017.11 (48621156..48626358, complement) |
Nucleus.
You may be wondering how to make the most of the HOXB9 cell-based ELISA kit from Boster Bio. The information provided here is intended for scientists and researchers. Boster scientists can submit their results for special samples, species, and applications, and receive product credits. All scientists can benefit from this information, including amateur scientists. You can find more information about Boster Bio at its website.
The HOXB9 marker is a unique biomarker that allows researchers to identify the expression profile of HOXB9 proteins. The HOXB9 cell-based ELISA kit is a highly efficient and convenient way to monitor the relative levels of HOXB9 in cells. Researchers can use the ELISA kit to screen for the effect of various treatment or activator molecules.
The HOXB9 Cell-Based ELIISA Kit from Boster Bio allows you to detect the protein HOXB9 and determine the effect of various stimulation conditions on HOXB9 expression in a variety of cell lines. Using Anti-HOXB9 antibodies, this kit captures HOXB9 and detects it with HRP-conjugated secondary antibodies. These secondary antibodies contain an enzyme called HRP that catalyzes a colorimetric reaction upon addition of substrate. This kit also allows you to perform multiple normalizations.
HOXB9 Cell-Based ELIISA Kit is an excellent choice for high-throughput screening. These kits provide quick results and are convenient for laboratory use. They are suitable for detecting changes in phosphorylation levels of HOXB9. LSBio ELISA kits are not finished goods; they are assembled and tested upon order. During this testing process, small changes may occur in the Range, Sensitivity, and Precision. However, significant changes will be communicated to the customer before shipping. LSBio ELISA kits are marked with a lot number that corresponds to the date of final assembly and testing.
This ELISA kit is useful for screening HOXB9 gene expression in tumor cells. Cancer cells respond to chemotherapy by regulating several mechanisms, including transforming growth factor-b2, which modulates the tumor stroma and promotes tumor growth and metastasis. HOXA9 also induces cancer-associated fibroblasts (CAMs), which mediate chemoresistance in cancer cells. It is also involved in maintaining NAC1-induced chemoresistance.
ELISA kits come with all the reagents needed to perform the experiment. This technique uses enzymes linked to antibodies to detect and quantify the presence of molecules in a sample. There are many formats of ELISA, and the principle of antigen-antibody interaction differs. Boster's Picokine(tm) ELISA kits utilize high-affinity antibodies that recognize native form proteins. Moreover, Boster's QC department validates its ELISA kits against relevant superfamilies and similar immunogenic proteins.
High-affinity primary antibodies targeting HOXB9, HOXD13, or HOXB9 were produced with boster bio's proprietary ELISA platform PicoKine(tm). These kits were tested for their sensitivity and specificity in IP and IHC. They also react with Human cells. They are supplied in a 100ul Liquid form.
The HOXB9 marker has been identified in a study involving 168 LSCC patients. In the study, HOXB9 expression was significantly associated with a poor prognosis and correlated with histological grade. This marker may serve as a new, more accurate, and more contextual marker of disease progression. Furthermore, it is highly compatible with various secondary antibodies.
These Boster high-affinity antibodies are highly specific. Using multiple controls, the researchers were able to validate their findings. If they were able to see only one band, it indicates that the antibody was specifically directed against the targeted protein. The results obtained from these experiments can be compared with the results from other tests and the results can be analyzed using IHC or Flow cytometry. Using negative controls in an IHC experiment is crucial, since it can reveal whether the secondary antibody bound non-specifically and thereby detect false-positives.
The HOX gene is a member of the HOX family of genes. It has been implicated in various solid tumors, including leukemia and prostate. Its expression level is highly dependent on the cell type. In this case, it is crucial to identify whether a particular protein is expressed in a tumor before starting treatment. These researchers have also discovered that HOXD13 is expressed in a broad range of tumor types, including a variety of squamous cell carcinomas.
Polyclonal antibodies are generated from the immune systems of their hosts. They contain an epitope, referred to as a complementarity determining region. Complementarity determining regions are complicated concepts. For more information, see Wikipedia's page on antibody complementarity determining regions. The method of producing polyclonal primary antibodies requires scientists to immunize host animals with antigens and then isolate the antibodies from the sera or eggs.
PMID: 11857506 by Kosaki K., et al. Complete mutation analysis panel of the 39 human HOX genes.
PMID: 2570724 by Giampaolo A., et al. Differential expression of human HOX-2 genes along the anterior- posterior axis in embryonic central nervous system.