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- Table of Contents
Facts about Helicase-like transcription factor.
These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein may bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
Human | |
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Gene Name: | HLTF |
Uniprot: | Q14527 |
Entrez: | 6596 |
Belongs to: |
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SNF2/RAD54 helicase family |
DNA-binding protein/plasminogen activator inhibitor 1 regulator; EC 3.6.1; EC 3.6.4.-; EC 6.3.2.-; helicase-like transcription factor; HIP116ARING finger protein 80; HIP116matrix associated, actin dependent regulator of chromatin; RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3; subfamily a, member 3; Sucrose nonfermenting protein 2-like 3; ZBU1
Mass (kDA):
113.929 kDA
Human | |
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Location: | 3q24 |
Sequence: | 3; NC_000003.12 (149030063..149086554, complement) |
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone.
Boster is a great option if you're looking for primary antibodies with high affinity to various applications. These antibodies are produced using proprietary technology and are available both for research and commercial use. Read on to learn more about Steven Boster and his biography. Boster Bio: Steven Boster is a well-known scientist and researcher. He was also referred to as the "science conversion" in the lavatory. He is well-known for creating more than 300 primary antibodies and has created one of the most efficient ELISA platforms.
Boster Bio offers high-affinity primary antisera that have been well verified and referenced in the research community. They are extremely specific and are suitable to perform immunoprecipitation as well as Western Blotting as well as ELISA assays. The products have been tested on a wide range of applications and they are backed by a guarantee of high quality is provided. Are you interested in a sample? Contact us now for more details.
In addition to providing high-affinity primary antibodies, Boster Bio offers custom antibody development services. Their expert team is highly skilled in developing custom antibodies and can create antibodies from chickens and rabbits. Other services they offer include humanization, antibody optimization, and custom Fc and Fab fragments. If you require a custom-made antibody, Biogenes will develop a monoclonal cell line for you. The process is about 0.2 mg protein and can take up to three months. Additionally, they offer contract services, including the purification of antibodies.
Boster Bio's anti-SARS CoV-2 monoclonal anti SARS CoV-2 antibody, VHH-72 is highly affine to the SARS-CoV-2RBD. The antibody binding test assessed their affinity to biotinylated RBD that is immobilized on magnetic beads. Based on three independent experiments the IC50 values were determined. These results are averages of three experiments.
Human samples were used to isolate monoclonal high-affinity anti-SARS-CoV-2 monoclonal monoclonal antibodies. These antibodies possess high affinity and might be worthy of further study in urgent COVID-19 applications. Single-B cell screenings can also be used to identify powerful neutralizing antibodies. They are extremely efficient in the production of monoclonal anti HEL-1 mAbs as well as high-affinity antibodies.
The globulins HEL-Fc and anti-HEL-1 can be used in a variety of ways. They are able to be used as key elements in experiments or in assays. Immunoassays depend on antibodies' ability to bind specific antigens. Some examples include Western Blotting, immunohistochemistry, and histopathology. Other uses of high-affinity primaries antibodies include flow cytometry which measures the amount of specific cell types.
Boster Bio has a reputation for producing high-quality antibodies ELISA kits, and reagents. Their products have received over 29,000 citations , and are rigorously tested. The range of products offered by Boster includes neuroscience, biomedical, and forensics. Every product comes with the Boster quality guarantee, which ensures optimal performance of ELISA/WB.
A panel of high-affinity primary antibodies is produced by the immune system and continues to develop as part of the ongoing immune response. Researchers have discovered a genetic limit to affinity maturation and the expansion of repertoire. They found that two adult volunteers had matured independently after three consecutive tetanus-toxoid booster vaccinations. The third vaccination did not significantly affect the distribution of somatic mutations, nor the binding rate constants of fully matured Abs. The variations remained the same in size, size, and dynamic and genetically diverse.
The second objective was to develop an mAb that was high-affinity hybridoma-derived against the hPCSK9. CDR-grafting was employed for humanization to reduce the immunogenicity. Then, the most important residues in the murine FRs were back-mutated so as to restore full affinity. Optimized scFvs were then fused to a human IgG1 constant region.
The Ab used in this study was raised to the target antigen and incubated at 37°C with PBST. After the incubation process, TMB substrate was added to the sample, which was allowed to develop for 15 minutes at temperatures at room temperature. Microplate readers were used to measure the absorbance at 450nm. All other reagents used were analytical grade. Bovine serum albumin is accessible on the website of the company.
The m5E12scFv was created using the CDRs of m5E12 and grafted onto human antibody chains using an GenBank accession number. After that, back-mutation took place on the m5E12scFv resulting in a highly specific antibody with longer lifespan than the parental antibody. In contrast the h5E12scFv was much more powerful than the parent antibody.
The anti-B strain antibody was able to interact with the new B strain with the same intensity and even higher affinity. Adaptation was not limited by the presence of previous exposure to B/Malaysia/2506/2004; however, pre-vaccination serum titers were below background levels. These results indicate that Boster Bio high affinity primary antibodies are highly specific against the latest strains of influenza.
The MBP-Linker scFv antibody showed high specificity for IFN-g and was completely incompatible with other antigens. The antibody has an affinity coefficient of 2.6x109 L/mol for IFN-g. The affinity constant is calculated based on the measured data. This antibody is extremely specific for IFN-g and thus providing effective results in diagnostic and research applications.
Steve Boster, who died June 6, 2022 Will be remembered for his generosity and kindness. Steve Boster, who was an Joliet, IL native, was a retail sales manager who was a lover of life and was proud to be a Concordia Hall member in Staunton, VA. He is survived by his two daughters Natosha and Crystal Boster, as well as his six grandchildren. He also leaves behind his mother, Frances, and siblings Jack, Sandra, and Tammy along with his nephews and nieces.
Steve Boster's 1993 IHC kit was his first product. After a successful development of hundreds of primary antibodies, Boster was known as "he who turns science into the toilet." Boster was China's leading supplier of antibodies in the late 1990s. Boster's innovations led to PicoKine(tm) which is a patent-pending ELISA platform. The proprietary trade secrets enabled Boster to create ELISA kits that have high sensitivity and high repeatability.
PMID: 7876228 by Sheridan P.L., et al. Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter.
PMID: 8672239 by Ding H., et al. Characterization of a helicase-like transcription factor involved in the expression of the human plasminogen activator inhibitor-1 gene.