GRK4 Antibodies

AND GRK4 ELISA kits, GRK4 Proteins

Many biological assays use antibodies to detect GRK4, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with GRK4 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop GRK4 antibodies...

We validate the specificity of these antibodies to GRK4 by testing them on tissues known to express GRK4 positively and negatively. Browse below to find the GRK4 antibody that suites your experiment. We have 4 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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GRK4 Infographic by Boster Bio

All GRK4 Products

Product

Figures

Specificity

Applications

Price

Anti-GRK 4 Antibody

Human

ELISA, IHC

$380
Cat # A04238-1
100ul

GRK4 (NM_182982) Human Recombinant Protein

$1249
Cat # PROTP32298
20 µg

Anti-GRK4 Mouse Monoclonal Antibody [Clone ID: OTI4F5]

Human

$523
Cat # M04238
100 µl

GRK4 (NM_182982) Human Over-expression Lysate

Human

$960
Cat # LS002868
100 µg

GRK4 Overview

Facts about G protein-coupled receptor kinase 4.

GRK4 3D structure. Source: alphafold

G protein-coupled receptor kinase 4 (GRK4)

Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; another three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin.

GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.

Gene Information

Human
Gene Name:	GRK4
Uniprot:	P32298
Entrez:	2868

Family

Belongs to:

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.16; G protein-coupled receptor kinase 2-like (Drosophila); G protein-coupled receptor kinase 4; G protein-coupled receptor kinase GRK4; GPRK2LIT11; GPRK4GRK4a; G-protein coupled receptor kinase 4; ITI1

Molecular Weight

Mass (kDA):
66.583 kDA

Genomic Context


Human

Location:	4p16.3
Sequence:	4; NC_000004.12 (2963504..3040760)

Tissue Specificity

Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium.

Subcellular Localizations

Cytoplasm. Cytoplasm, cell cortex. Both at the cell surface and dispersed in the cytoplasm under basal conditions. Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells.

Diseases

Hypertensive Disease
Essential Hypertension
Familial Hypertension
Malignant Neoplasms
Cardiovascular Diseases
Cholangiocarcinoma
Neoplasms
Heart Failure
Dysequilibrium Syndrome
Microsatellite Instability
Colorectal Neoplasms
Infarction

Carcinogenesis
Myocardial Infarction
Cerebrovascular Accident
Colorectal Cancer
Renal Hypertension
Obesity
Kidney Diseases

Pathways

Excretion
Transport
Localization
Receptor Internalization
Natriuresis
Pathogenesis
Hyperphosphorylation
Renal Sodium Excretion
Endocytosis
Mismatch Repair
Rna Interference
Granulocyte Differentiation
Gene Silencing

Fertilization
Diuresis
Nuclear Export
Reverse Transcription
Chemotaxis
Lymphocyte Chemotaxis
Lipid Binding

PTMs

Phosphorylation
Palmitoylation

Prenylation
Methylation

Research Areas

Protein Kinase

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/GRK4

References

PMID: 1338872 by Ambrose C., et al. A novel G protein-coupled receptor kinase gene cloned from 4p16.3.

PMID: 9092566 by Sallese M., et al. G protein-coupled receptor kinase GRK4. Molecular analysis of the four isoforms and ultrastructural localization in spermatozoa and germinal cells.