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- Table of Contents
Facts about Gap junction alpha-3 protein.
Human | |
---|---|
Gene Name: | GJA3 |
Uniprot: | Q9Y6H8 |
Entrez: | 2700 |
Belongs to: |
---|
connexin family |
Gap junction alpha-3 protein
Mass (kDA):
47.41 kDA
Human | |
---|---|
Location: | 13q12.11 |
Sequence: | 13; NC_000013.11 (20138252..20161327, complement) |
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction.
This article describes the advantages of using the GJA3 Marker, high-affinity primary antibodies from Boster Bio, and Steven Boster's history. Read on to discover how you can make the most of these antibodies and how they benefit you. If you are a scientist and want the best out of these antibodies, keep reading! These are the best uses of the GJA3 marker.
Four primer pairs are required for the pcr amplification of the GJA3 genetic gene. The point mutation is located at 427th base of the 1st exon gene. This mutation is needed to detect a potential transgene. GJA3 markers offer many benefits. Read on to find out more. The GJA3 marker was first discovered in 2003 and is available for purchase at various genetic testing laboratories.
Flowcytometry is a method that analyzes cell populations and particle communities. This is possible only if you have high-affinity prim antibodies. Boster stocks both monoclonal as well as polyclonal antibodies in its extensive catalog. These antibodies have been highly cited for more than 25 years and are a great choice for many scientific applications. Boster Bio's high-affinity primary antibodies are highly effective in a wide variety of applications, including tissue engineering and cancer research.
Boster Bio provides high-affinity, primary antibodies that are suitable for use with mouse or human samples. Boster Bio's extensive antibody collection includes over 12,000 antibodies, which have been validated for WB and ELISA as well as FC. Boster Bio's antibodies are quantitatively validated by testing against known amounts of recombinant protein, tissues, or untransfected cell line.
Antibodies with high affinity allow you to accurately measure the concentration and wash away non-specific binding materials. Antibodies that have high affinity are so specific they can be used to measure specific substances in crude preparations. Moreover, they are highly versatile, allowing you to conduct multiple tests with a single antibody. High-affinity antibodies can also be used to measure the concentration of target antigens in a short time.
Single-cell screening allows for the isolation of high-affinity antibodies that are specific against the target antigen in a matter of weeks. This method isolates viable cells from convalescent animals and immunized humans. The isolated cells are then sorted by single-cell sorting at high throughput to isolate VH/VL antigen genes. Next, they will be screened and evaluated for their neutralizing potential.
When your immune system detects a small amount of antigen, it triggers B cells to respond. High-affinity receptors are more able to compete against preexisting antibodies and present fragments of peptide to armed T cells. Both helper T cells as well as B cells will eventually multiply rapidly. During the secondary immune reaction, only the memory B cells with higher affinity can be stimulated.
The affinity maturation limits were determined at physiological temperatures, and a representative set of 50 Abs was used from the last two vaccinations. The data were arranged around clearly defined mean values. The geometric mean for kon remained the same, while that for koff increased by 2.4-fold. This corresponds to a decrease in the average interaction, t1/2. These data are crucial for your research.
ELISA tests can be performed during antibody development. The antibodies are then incubated alongside biotinylated SARS-2RBD immobilized with magnetic beads. The binding assays showed that both high affinity and neutralizing antibodies bound the SARS-2 RBD in vivo. These results are a composite of three independent experiments.
If you are interested to learn more about Steve Boster's past, you have come to the right spot. PicoKine(tm), a company-owned ELISA platform, provides a comprehensive database of public records that includes Steve Boster’s current and past addresses, mobile numbers, email addresses, and known family members. You can filter the results according to state and age to find out more about Steve Boster.
Steve Boster passed away on June 26, 2022. He was born in Joliet, IL, and passed away in Madison, WI, after a long battle with COVID-19. His children were Donald Sr., Nina Mae Hall and David Boster. Crystal Boster is survived by two sisters, Kimberly Blanton (and Tammy Boster), as well several nieces/nieces.
PMID: 10205266 by Mackay D., et al. Connexin46 mutations in autosomal dominant congenital cataract.
PMID: 10746562 by Rees M.I., et al. Further evidence of autosomal dominant congenital zonular pulverulent cataracts linked to 13q11 (CZP3) and a novel mutation in connexin 46 (GJA3).