FZR1 Antibodies

Fizzy-related protein homolog (FZR1)

Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase.

The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle don't accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C.

Gene Information

Human
Gene Name:	FZR1
Uniprot:	Q9UM11
Entrez:	51343

Family

Belongs to:
WD repeat CDC20/Fizzy family

Alternative Names

CDC20C; CDH1; Cdh1/Hct1 homolog; CDH1CDC20-like 1b; fizzy/cell division cycle 20 related 1 (Drosophila); Fzr; FZR2; FZRCDC20-like protein 1; hCDH1; HCDHFYR; KIAA1242fizzy-related protein homolog

Molecular Weight

Mass (kDA):
55.179 kDA

Genomic Context

Human
Location:	19p13.3
Sequence:	19; NC_000019.10 (3506263..3538334)

Tissue Specificity

Isoform 2 is expressed at high levels in heart, liver, spleen and some cancer cell lines whereas isoform 3 is expressed only at low levels in these tissues.

Subcellular Localizations

[Isoform 2]: Nucleus.; [Isoform 3]: Cytoplasm.

Diseases

• Neoplasms
• Carcinoma
• Malignant Neoplasms
• Cell Transformation, Neoplastic
• Tissue Adhesions
• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Endoreduplication
• Genomic Instability
• Neoplasm Metastasis
• Melanoma
• Aneuploidy

• Infertility
• Renal Cell Carcinoma
• Malignant Paraganglionic Neoplasm
• Neurodegenerative Disorders
• Carcinogenesis
• Alzheimer's Disease
• Stomach Neoplasms

Interacting Proteins

• CDC27
• CLSPN
• MAD2L2
• MAK
• RAD17

• RRM2
• SKP2
• VHL

Pathways

• Cell Cycle
• Mitosis
• Anaphase
• Meiosis
• Prophase
• Meiosis I
• Spindle Assembly
• Cell Division
• Cell Proliferation
• Proteolysis
• Spindle Assembly Checkpoint
• Metaphase
• Cell Differentiation

• Dna Replication
• Meiosis Ii
• Localization
• Exit From Mitosis
• S Phase
• Chromosome Segregation
• G1 Phase

PTMs

• Phosphorylation
• Ubiquitination

• Methylation
• Farnesylation

• Deacetylation

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FZR1

Best Uses Of The FZR1 Marker

If you are searching for the best antibody available to detect Fizzy related protein homologs, you have come to right place. Boster Bio offers the best ELISA kits for FZR1. Boster Bio also offers custom services and BeNeLux delivery. Bosterbio.com/fzr1 can be used to purchase this product.

Homolog for Fizzy-related protein homolog

This antibody detects FZR1, which is the mouse ortholog of Fizzy's related protein. It is ideal for IHC, IF, and WB applications. It reacts to both Human and Mouse protein. APC–Cdc20 is a substrate specific adapter protein that activates the APC/C during anaphase. It reacts with FZR1, which makes it useful for detecting FZR1 from various biological samples.

Adapter for anaphase promoting complex/cyclosome E3 ubiquitin protein ligase complex

The APC/C is a core component of the mitotic checkpoint and is activated during mitosis by the CDC20 substrate adaptor protein. APC/C activation requires phosphorylation of CDC20. However, this is not fully understood due to its many phosphorylation locations. The complexity of activating APC/C due to the numerous mutations in this complex makes it difficult to perform systematic molecular mutagenesis.

The APC/C complex is composed of at least twelve subunits, which recruit a host of substrates for degradation by the proteasome. These proteins are essential to the progression of the cell's cycle, including the divisions of the chromosomes. APC/C facilitates proper microtubule attachement to chromosomes by kinetochoromes. This is essential for the proper congress of the chromosomes to the metaphase plate.

The APC/C member of the RING family is an E3 ubiquitin ligase. The RING is a family of proteins that have two substrates. These substrates act as a scaffold to the E3 ubiquitin ligase-protein ligase compound. The APC/C-complex catalyzes the transfer of ubiquitin by joining a K11 linked ubiquitin chain.

The APC/C is a heterodimer. It uses two E2 Families to regulate its activity. Conformational changes of Apc8B domains and C-boxdomains are believed to mediate activation. This results in a catalytically-friendly conformation, where E2Ub is loaded into the APC/C.

APC/C (multi-subunit ubiquitinprotein ligasae) regulates cell cycle via binding mitoticallyphosphorylated Cdc20. The APC/C has multiple sites phosphorylated by Cdk1, but these sites have not yet been identified. Site-directed mutation has revealed the mechanism for Cdk1 phosphorylation in APC/C, which triggers the onset of anaphase.

NBs with an APC/C mutant are characterized by an accumulation of Miranda pericentrosomal in location and a reduced basally localised protein. Mutants in centrosomin which is a component APC/C, have normal Miranda location and anaphase promoting complex/cyclosome complex (APC/C E3 Ubiquitin-protein Ligase complex) Miranda accumulation in the pericentrosomal regions. NBs with an intact centrosomal function did not accumulate Miranda in the pericentrosomal region.

Monoclonal antibody

Boster Bio can provide you with an anti FZR1 antibody. The company offers several high-affinity antibodies that have been validated in Western blotting, Immunohistochemistry, and ELISA. The company offers anti FZR1 products to meet your research needs. Here are some of the best options:

First, take a look at the name. Its main function, however, is to recognize BARD1, BCL2L1 or atopaxin. The cellular homing receptor BARD1 is part of the BCR protein family. Its other names are EZH2, EG5, hHR6A. It is also associated HIVEP3/KRC or HMGA1.

For research purposes, polyclonal antibodies can be acceptable. They are often the first step towards creating a monoclonal antigen. Although any antigen can create polyclonal immune responses, this approach doesn't allow the amplification and enhancement of the best clones. Polyclonal antibodies are typically used to detect secretive proteins and biomarkers. Polyclonal antibodies bind to a target protein with a higher affinity and low cross-reactivity.

While WB is the initial validation step, WB results can't serve as absolute evidence of a monoclonal antibody's specificity. IHC and flowcytometry can also be used to validate the specificity of monoclonal antibodies. These techniques also benefit from negative controls, which allow you to determine whether secondary antibodies are interacting with the antigens in a non-specific manner or creating false positives.

Picogram sensitivity ELISA kit

The Picokine ELISA kits are proprietary formulations that achieve high sensitivity at a picogram level and low background. These kits are tested thoroughly and documented. This allows for consistency between batches and transparency in sharing validation data. They also go through extensive screening, quality control, laboratory testing, and a lot more. You can be confident in the quality of these kits. Picokine ELISA kits offer many benefits.