Exonuclease V (EXO5)

Single-stranded DNA (ssDNA) bidirectional exonuclease involved in DNA repair. Probably involved in DNA repair following ultraviolet (UV) irradiation and interstrand cross-links (ICLs) damage.

Has both 5'-3' and 3'-5' exonuclease activities with a strong preference for 5'-endings. Acts as a sliding exonuclease that loads at ssDNA ends and then slides across the ssDNA prior to cutting; however the sliding and the 3'-5' exonuclease activities are abolished upon binding to the replication protein A (RPA) complex that enforces 5'-directionality activity.