D-aminoacyl-tRNA deacylase 1 (Dtd1)

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl- tRNA(Ala), protecting cells from glycine mischarging by AlaRS.

Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids at the active site. By recycling D-aminoacyl-tRNA into D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps apply protein L-homochirality.