Dynamin-2 Antibodies

Dynamin-2 (DNM2)

Microtubule-associated force-producing protein involved in producing microtubule bundles and can bind and hydrolyze GTP. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity).

Plays an important role in vesicular trafficking processes, in particular endocytosis. Involved in cytokinesis (PubMed:12498685).

Gene Information

Human
Gene Name:	DNM2
Uniprot:	P50570
Entrez:	1785

Family

Belongs to:
TRAFAC class dynamin-like GTPase superfamily

Alternative Names

CMTDI1CMT2M; CMTDIBdynamin II; DI-CMTB; DYN2EC 3.6.5.5; dynamin 2; dynamin-2; DYNII

Molecular Weight

Mass (kDA):
98.064 kDA

Genomic Context

Human
Location:	19p13.2
Sequence:	19; NC_000019.10 (10718053..10831910)

Tissue Specificity

Ubiquitously expressed.

Subcellular Localizations

Cytoplasm. Cytoplasm, cytoskeleton. Cell junction. Membrane, clathrin-coated pit. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Midbody. Cell projection, phagocytic cup. Cytoplasmic vesicle, phagosome membrane; Peripheral membrane protein. Colocalizes with CTTN at the basis of filopodia in hippocampus neuron growth zones (By similarity). Microtubule-associated. Also found in the postsynaptic density of neuronal cells. Co-localizes with PIK3C3 and RAB5A to the nascent phagosome (By similarity).

Diseases

• Myopathy
• Congenital Structural Myopathy
• Charcot-marie-tooth Disease
• Weakness
• Congenital Myopathy (disorder)
• Muscle Weakness
• Peripheral Neuropathy
• Blepharoptosis
• Ophthalmoplegia
• Atrophy
• External Ophthalmoplegia
• Nervousness

• Neuromuscular Diseases
• Muscle Hypotonia
• Malignant Neoplasms
• Hypertrophy
• Demyelinating Diseases
• Inherited Neuropathy
• Hereditary Motor And Sensory Neuropathies

Interacting Proteins

• AHI1
• CTTN
• GRB2
• ITSN1
• MYO1E

• NME2
• PACSIN2
• SH3BP4
• SH3GL2
• SH3KBP1

• SNX9

Pathways

• Endocytosis
• Clathrin-mediated Endocytosis
• Transport
• Localization
• Myelination
• Pathogenesis
• Rna Interference
• Autophagy
• Spermatogenesis
• Nuclear Transport
• Myelin Maintenance
• Translation
• Interphase

• Exocytosis
• Meiosis
• Receptor-mediated Endocytosis
• Membrane Tubulation
• Vesicle Transport
• Dna Repair
• Glomerular Filtration

PTMs

• Acylation

Research Areas

• Apoptosis
• Membrane Trafficking and Chaperones
• Neuronal Cell Markers
• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DNM2

Best Uses Of The DNM2 Marker From Boster Bio

The DNM2 marker can be used to determine DNM2 activities in biological samples. This biomarker is an organic substance found in liver cells. It is part of the cytokine family, and is abundant in many tissues and cells. Boster Bio's DNM2 antibody is a good choice to detect DNM2 activity in many different biological samples.

Membrane staining increases protein transfer efficiency

Boster Bio's Protein Transfer System utilizes an Immobilon-P membrane or a nitrocellulose membrane PVDF as a medium for transport. The proteins are transferred at an 80mA in the Tris–glycine buffer. This buffer contains 48 mM Tris and 39 Glycine. It also contains 0.037 percent SDS and 20 Methanol. Protein bands can be detected using Coomassie Brilliant Blue R-250 dye.

Every protein has a unique transfer efficiency. It depends on the ability of the protein to move out of the gel, and then bind to the membrane, composition of the buffer and place of the electrodes. The composition and size, strength of the electric field, and presence of detergents and alcohol in the buffer all affect the transfer efficiency. To maximize the efficiency of transfer you should use a gel with high efficiency that has high rates of transfer of proteins.

Alternately you can use an autoradiography film to record the results in the darkroom. Based on the molecular weight of the proteins, the pore size of the NC membrane has to differ. Typically, NC membranes are used in the range of 0.45 um to 0.2 um. For proteins that have a maximum of 20KD it is recommended to use the NC membrane is suggested. For small molecular weight proteins make use of a PVDF membrane. This material is more sensitive resolution, resolution, and also a higher affinity.

Boster Bio Protein Transfer System's main antibody is highly reactive to a vast range of cytotoxic agents. For instance, if are looking at the transfer efficiency of antigens, you should be looking for a signal to indicate the presence of antigen on the membrane. If you're unable to find an antigen, you might need to use secondary antibodies. For this purpose, BosterBio has several other options.

It is easy to identify the presence of proteins on membranes. However, there are numerous problems that come with wet transfer. Wet transfer requires lots of effort and time. Semi-dry blotting is easy, but it's not as efficient than wet. Semi-dry blotting is also delicate, and can erase small molecular proteins once the membrane is washed. PVDF and nitrocellulose membranes, however are more durable.

ECL chemiluminescent detection system

A chemiluminescent detection system records the light signal generated by an enzymatic reaction within the membrane. The enhanced chemiluminescent substrates of the system (ECLs) emit light at 425nm. They are typically two-component systems. The light is only emitted during the reaction between the target and enzyme. Signal output ceases after the enzyme has exhausted all substrates

The C-DiGit (r) Blot Scanner eliminates the necessity for film, darkroom expenditures and development reagents. Furthermore, the CDGit blot scanner's high dynamic range means fewer exposures are necessary for optimal results. The iBright Imaging system uses a 9.1MP cooled CCD camera that has a high sensitivity as the dynamic range.

The Clarity Max substrates are compatible with any horseradish peroxidase conjugate. These ECL detection systems are perfect to western blot experiments that require abundant proteins. They are also highly sensitive and suitable for optimization studies. The Clarity Max is ideal for western Blot applications. The name suggests that they are designed to enhance the sensitivity of detection of proteins.

The SuperSignal ECL substrates manufactured by Thermo Scientific offer excellent western blotting performance. Both substrates can be used with a range of labeling systems. Additionally, the SuperSignal ECL substrates can be used on nitrocellulose membranes. There are a myriad of SuperSignal ECL substrats that are available. Select the one best suited to your needs for experimentation.

DAB chromogenic detection device

The DAB chromogenic detection method from Boster Bio allows for the visualisation of antibodies in samples. This method makes use of an enzyme that converts a substrate that is soluble into an insoluble, colored product that is deposited on the site of antigen expression. The enzymes commonly used are horseradish peroxidase. These enzymes convert an insoluble compound called 3,3'-diaminobenzidine into a colored substance that is able to be detected by the system. The enzymes are generally biotin-based , and employ an avidin-biotin complex to detect the antigen. Another method is the use of a micropolymer or a polymer complex that reacts with the antigen.

Boster Bio DAB chromogenic Substrat Kit is used to chromatograph cells and sections. This kit has a long shelf-life and is recommended for use in immunohistochemistry and the cytochemistry. It will last for a year at -20 degrees Celsius. In addition to Boster Bio's BCIP/NBT Chromogenic Substrate Kit, you also have to purchase an option that has nitro-blue Tetrazolium.