Homeobox protein DLX-5 Antibodies

Homeobox protein DLX-5 (DLX5)

Transcriptional factor involved in bone growth. Acts as a direct early BMP-responsive transcriptional activator essential for osteoblast differentiation.

Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation.

Gene Information

Human
Gene Name:	DLX5
Uniprot:	P56178
Entrez:	1749

Family

Belongs to:
distal-less homeobox family

Alternative Names

distal-less homeo box 5; distal-less homeobox 5; DLX5; homeobox protein DLX-5

Molecular Weight

Mass (kDA):
31.54 kDA

Genomic Context

Human
Location:	7q21.3
Sequence:	7; NC_000007.14 (97020396..97024831, complement)

Subcellular Localizations

Nucleus.

Diseases

• Crest Syndrome
• Rett Syndrome
• Craniofacial Abnormalities
• Hypertrophy
• Nervousness
• Osteopenia
• Autistic Disorder
• Neoplasms
• Limb Deformities, Congenital
• Malignant Neoplasms
• Congenital Abnormality
• Congenital Foot Deformity

• Fracture
• Congenital Hand Deformities
• Cleft Palate
• Ectrodactyly
• Lymphoma
• Craniosynostosis

Pathways

• Osteoblast Differentiation
• Ossification
• Cell Proliferation
• Regeneration
• Bone Development
• Cell Differentiation
• Pathogenesis
• Methylation
• Chondrocyte Differentiation
• Ear Development
• Neurogenesis
• Dna Methylation
• Forebrain Development

• Limb Development
• Endochondral Ossification
• Inner Ear Development
• Bone Resorption
• Chromatin Remodeling
• Localization
• Cell Death

PTMs

• Phosphorylation
• Methylation
• Demethylation
• Sulphation

• Sumoylation
• Reduction
• Hydroxylation
• Cleavage

• Ubiquitination

Research Areas

• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DLX5

Best Uses Of The DLX5 Marker

DLX5 Cell Based ELISA Kit measures DLX5 expression in cells. It is a fast and easy to use assay. Using this kit, you can monitor the effects of treatment or activators on DLX5 protein levels in cells. This kit is only available for Boster products and is not available individually. These are just a few of the many uses that this kit can be used for.

DLX5 Cell Based ELISA Test Kit

The DLX5 ELSA Kit Cell Based is a simple and fast assay for measuring DLX5 levels in cells. It can be used for screening for the effects of different inhibitors and treatments on DLX5 gene expression. The ELISA Kit remains stable at -20°C and 4°C for six months.

Boster Bio DLX5 Cell based ELISA Kit measures DLX5 Protein levels in cells. It is compatible with most cell types and mediates the result in the form of a real-time, automated reaction. It can also detect a wide variety of metabolites. DLX5 a novel cancer drug with anti-leukemia properties. It has been shown that it affects the leukemogenic PI3K/Akt signaling pathway.

DLX5 Marker in Down syndrome

A new study suggests that the DLX5Marker could be a potential therapy for Down syndrome. This study was performed in mice and showed that Down syndrome patients had altered neuronal activity of two classes inhibitory cells in their brains. These cells regulate neuronal activity and are associated for impaired cognitive function. The authors propose that this altered activity might influence the activity of cortical networks in Down syndrome patients.

SPSS and GraphPad Prism 5.0 were used for this study. We performed Mann-Whitney's U-tests to compare continuous and categorical variables. Spearman test was used to examine the correlation of DLX5 methylation with expression. To determine the association between methylation of DLX5 and its expression, we used the receiver operating characteristic (ROC), curve. The area under ROC curve was used as a measure of effectiveness.

DLX5 cell-based ELISA kit for Down syndrome

The DLX5 Cell Based ELSA Kit is a simple and efficient assay to determine DLX5 proteins expression in cells. This kit measures the relative levels of DLX5 protein in cultured cells. It is a great screening tool for studying drug treatments and activators. This kit is only compatible to Boster products and is not available separately.

The ELISA Kit and the PCR mastermix (Applied Biosystems) were used to determine the DLX5 level. The PCR conditions for the ELISA kit were 95°C, 60°C, for 60 s. A 4-fold dilution standard curve and 95°C was used. The average of two separate PCRs using different amounts RNA were used to calculate the values for each sample. The DLX5 cell based ELISA kit for Down syndrome was developed using primer sequences that target human DLX5 (and its complement receptor, NOTCh2) as well as a 6FAM probe.

MYC Promoter is the binding location for DLX5. Mutations at the D1 and D4 bindings sites inhibit MYC transcriptal activation. In order to measure MYC's activity, the MYC promoter was transfected into DLX5 cellular cells. The D4 binding site mutation inhibited MYC promoter activity, but the D1-M1 mutation did not have any effect.

DLX5 is a powerful screening tool for Down Syndrome. Transient transfection of the gene-targeting siRNA caused a 3.5-fold increase cell proliferation in human lung cancer cells. The siRNA transfection of non-targeting siRNA reduced MYC expression to 80%. Transfection efficiency almost 100% when siRNA targeting MYC are transfected into cells containing DLX5.

The DLX5 cell-based ELSA Kit in Down syndrome is available at many hospitals and has many applications. Molecular diagnosis of Down syndrome is a vital step in diagnosis and treatment. It is vital to detect the genetic defect in time to monitor the disease's progress. The DLX5 gene is a gene that controls regional development in the branchlets.

Patients with Down syndrome have a poor prognosis due to DLX5-overexpression. It is associated with a poor prognosis among patients with nonsmall cell lung carcinoma (NSCLC). Specific siRNA against DLX5 prevents the proliferation NSCLC cells. If you are concerned about the likelihood of developing Down Syndrome, you can order a DLX5 Cell Based ELISA Kit.

The DLX5 gene is important in the development of bone tissue. DLX5 activates RUNX2 proteins in the bone morphogenetic pathway. In addition to this, DLX5 promotes osteoblast maturation. Mice lacking DLX5 have severe malformations and a delay in ossification. DLX5 may be a candidate gene that regulates bone formation and fracture repair.