DHX9 Antibodies

ATP-dependent RNA helicase A (DHX9)

Multifunctional ATP-dependent nucleic acid helicase that unwinds DNA and RNA in a 3' to 5' direction and that plays important roles in many processes, such as DNA replication, transcriptional activation, post-transcriptional RNA regulation, mRNA translation and RNA-mediated gene silencing (PubMed:9111062, PubMed:11416126, PubMed:12711669, PubMed:15355351, PubMed:16680162, PubMed:17531811, PubMed:20669935, PubMed:21561811, PubMed:24049074, PubMed:25062910, PubMed:24990949, PubMed:28221134). Requires a 3'-single-stranded tail as entrance site for acid nuclei unwinding activities in addition to the binding and hydrolyzing of some of the four ribo- or deoxyribo- nucleotide triphosphates (NTPs) (PubMed:1537828).

Unwinds numerous nucleic acid substrates like double-stranded (ds) DNA and RNA, DNA:RNA hybrids, DNA and RNA forks composed of partially complementary DNA duplexes or DNA:RNA hybrids, respectively, and also DNA and RNA displacement loops (D- and R-loops), triplex- helical DNA (H-DNA) structure and DNA and RNA-based G-quadruplexes (PubMed:20669935, PubMed:21561811, PubMed:24049074). Binds dsDNA, single-stranded DNA (ssDNA), dsRNA, ssRNA and poly(A)-containing RNA (PubMed:9111062, PubMed:10198287).

Gene Information

Human
Gene Name:	DHX9
Uniprot:	Q08211
Entrez:	1660

Family

Belongs to:
DEAD box helicase family

Alternative Names

DDX9ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9 (RNA helicase A, nuclear DNAhelicase II; leukophysin); DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; EC 3.6.1; EC 3.6.1.15; EC 3.6.4.13; FLJ17406; leukophysin; LKP; NDH II; NDH2; NDHII; Nuclear DNA helicase II; RHA

Molecular Weight

Mass (kDA):
140.958 kDA

Genomic Context

Human
Location:	1q25.3
Sequence:	1; NC_000001.11 (182839347..182887982)

Subcellular Localizations

Nucleus. Nucleus, nucleoplasm. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleoplasmic shuttling protein (PubMed:10198287, PubMed:16375861, PubMed:10207077, PubMed:9162007). Its nuclear import involves the nucleocytoplasmic transport receptor Importin alpha/Importin beta receptor pathway in a Ran-dependent manner (PubMed:16375861). In interphase, localizes in nuclear stress granules and at perichromatin fibrils and in cytoplasmic ribonucleoprotein granules (PubMed:10198287). Colocalizes with WRN and H2AX at centrosomes in a microtubule-d

Diseases

• Malignant Neoplasms
• Neoplasms
• Immunologic Deficiency Syndromes
• Lupus Erythematosus, Systemic
• Hiv Infections
• Malignant Neoplasm Of Breast
• Chromosomal Translocation
• Osteosarcoma
• Cell Transformation, Neoplastic
• Virus Diseases
• Autoimmune Reaction
• Leukemia

• Werner Syndrome
• Sarcoma
• Carcinoma
• Mammary Neoplasms
• Rheumatoid Arthritis
• Influenza
• Autoimmune Diseases

Interacting Proteins

• EIF2AK2
• NXF1
• PRMT1
• RELA
• XPO1

• YBX1
• ZMAT3

Pathways

• Localization
• Rna Processing
• Transport
• Translation
• Nuclear Transport
• Gene Silencing
• Nuclear Export
• Dosage Compensation
• Dna Repair
• Methylation
• Cell Proliferation
• Cellular Localization
• Pathogenesis

• Aging
• Cell Cycle
• Mrna Stabilization
• Nuclear Import
• Rna Splicing
• Drug Resistance
• Regulation Of Translation

PTMs

• Phosphorylation
• Cleavage
• Methylation

• Biotinylation
• Acetylation
• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DHX9

The Best Uses Of The DHX9 Marker

If you want to find out how the DHX9 marker functions and what its applications are this article will prove beneficial. We'll review its function and its specificity, then we'll discuss its applications. If you're a scientist, check out these tips and information that will assist you in getting the most from this marker. These are the most important points to consider when evaluating the DHX9 mark.

DHX9

In addition to its function in DNA repair, DHX9 has also been demonstrated to be involved in maintaining the genomic stability. It is closely related to the gene gH2AX that is a key factor in NHEJ-mediated genome repair. DHX9 interacts with gH2AX through its helicase-helicase core domain and has been observed to accumulate in nuclear bodies adjacent to the gH2AX focal points.

DHX9 is a helicase which unwinds double-stranded RNA. It prefers substrates with short 3' tails and translocates from 3' to 5'. It utilizes all dNTPs and RNTPs, and is capable of unwinding DNA and RNA hybrids. It is not equipped with the Q-motif, which is responsible for many functions.

The DHX9 gene is expressed in many tissues and is often linked to cell's rRNA production. The presence of the gene in the nucleus indicates it is involved in a post-transcriptional process. It could also fulfill an the RNA-surveillance checkpoint. It may induce RNA-protein and RNARNA rearrangements and may even stimulate ribosome recycling.

The DHX9 gene regulates transcription, translation and microRNA biogenesis. It is involved in many human diseases and treatments. This review will provide a brief overview of DHX9 biochemistry and structure as well as the potential applications of this gene for drug discovery. Multidisciplinary approaches are essential to achieve the goal of better understanding and treating illnesses and conditions. So what can DHX9 be utilized in clinical studies?

DHX9 interacts with LMX1B it is a LIM homeodomain transcription component, in addition to its role as an expression regulator. This transcription factor is essential for the development of dopaminergic neuronal cells in the mesodiencephalon. This gene is crucial for the regulation of genes in a variety of diseases and is involved in various cell functions. The DHX9 gene is extremely conserved across all species of humans. It is highly conserved within human species due to its extended RGG-box and overlap nuclear localization/export signal.

Functions

DHX9 is a gene found on the long arm of chromosome 1. The N-terminus in the gene's core domain is home to two double-stranded domains for RNA binding. The minimal transactivation domain is next to the helicase core and allows interaction with RNA polymerase 2. The nuclear localization and export signals are in sync with those of the other DHX9 gene family members.

During development during development, during development, the DHX9 protein is expressed in the nucleus and shuttles to the cytoplasm to facilitate processing of miRNA and translational regulation. It is released from the nucleus during the process of mitosis. It first appears during prophase of the cell cycle, which is marked by chromosomal condensation and the breakdown of the nuclear envelope. In metaphase, DHX9 disappears from the nucleus, and is reinstated in the telophase.

The inhibition of the DHX9 interaction with the ETS transcription factor family provides potent anti-tumor effects. Inhibition of DHX9 activity reduces the growth and progression of several kinds of cancer. While there isn't any conclusive evidence linking DHX9 and prostate carcinogenesis (prostate cancer) and binding of AR to DHX9 promoter has been associated with bone metastasis, osteolytic formation, as well as the growth of DHX9 activity. Although the precise mechanisms behind this interaction aren't fully understood however, it is likely that it will occur in prostate cancer.

While DHX9 is a helicase it is also a strand-specific DNA/RNA resolution enzyme. It prefers triplex DNA structures with the single-strand tail of 3 and displaces the third strand. These strands could be unwound by DHX9. While DHX9 is an efficient helicase, it is generally structure-specific, preferring complex nucleic acid structures.

Specificity

DHX9 is an unwinding enzyme that can be found in both DNA and RNA. It prefers substrates with short 3' tails, which may be an anchor to bind the enzyme. It also prefers multi-stranded Nucleic Acid complexes. It is therefore essential to differentiate between DHX9 protein and an one-way protein. This can lead to inaccurate gene expression. This article will concentrate on the specificity and the purpose of the DHX9 marker.

The biochemical properties of DHX9 are comparable to those of the SV40 large T-antigen. It is also similar to RecQ Helicases which play a vital role in maintaining the stability of genomics. They break DNA structures, like D-loops and R-loops. This makes DHX9 an ideal candidate for the development of a genetic test for multiplex chromosome analysis.

Studies on molecular biology have demonstrated that DHX9 is linked to DNA damage sites, and that the localization of DHX9 for these sites is highly specific. Furthermore, the protein is in contact with the polycomb group protein PHF1, a protein that binds to double-stranded breaks. Although DHX9 can be used to detect chromosomal abnormalities in the chromosomal region, it is not recommended for routine use in the clinical setting.

In addition to regulating CREB-dependent transcription, DHX9 may also play an important role in transcription of transcripts. In addition, it's a bridging factor between CREB as well as RNA polymerase II. CREB is a promoter-specific molecule that interacts with specific genes with the cAMP responsive element, and DHX9 activates the RNA polymerase I to the CREB/CBP/p300 complex.

Immunoblotting of extracts of total cells was used to regulate HA-DHX9 transgene expression. DnsP4 chikV replicons were transfected into parental (WT) and HEK293T cells. After eight hours of transfection cells lysates could be examined. The intensity of each band was determined using ImageJ software. The intensity of the DHX9 bands was then normalized to GAPDH signals , and then expressed as a percentage for GFP condition.

Applications

The DHX9 gene encodes an RNA- and DNA- G-quadruplexes that unwind enzymes. It is also known that DHX9 can unwind D-loops and RNA forks. These enzymes can speed up transcription by resolving the abnormal structure. The first evidence of the role played by DHX9 was from Drosophila. MLE, DHX9's protein product that regulates dosage compensation is expressed by both genders, and bound to hundreds on the X the chromosome.

The DHX9 gene encodes a protein with an N-terminal helicase region. This domain spans amino acid residues 380-830, which is for humans. It also has two double-stranded RNA binding domains. Molecular analysis of DHX9’s interaction with RNA indicates that it interacts both with RNA polymerase II, rRNA, and the nuclear location and export signal. The DHX9 gene also contains an RGG-box containing glycine that is able to bind a single-stranded nucleotide binding fold.

For the association of the protein with PCE, the NTPase/helicase activation of DHX9 must be activated. DHX9 is associated with PCE in both the cytoplasm and nucleus, and thus, DHX9 could serve as an RNA surveillance checkpoint in cells. In addition, DHX9 can trigger RNA-protein rearrangements, which may enhance the rate of protein production. Furthermore the DHX9 gene can stimulate the process of recycling ribosomes.

The DHX9 gene has an extremely high degree of conservation across species. Human DHX9 shares 93 percent of its homology with the murine and bovine, mouse and Drosophila homologues. However, a significant portion of its homologs are shared by bovine, mouse and C. elegans. Sequence alignments were generated using BoxShade and T-Coffee.

Available

DHX9 is a protein that interacts with the ETS transcription family and modulates it activity. The activation of DHX9 boosts the transcription of genes targeted by. Ewing sarcoma cells grow less when DHX9 is depleted in PC cells. DHX9 inhibition reduced tumor growth and increased sensitivity for treatments. Additionally, DHX9 helicase activity prevents genomic instability and aids in maintaining DNA integrity.

DHX9 has multiple functions, including transcriptional regulation and DNA replication, as well as genome stability. It is expressed in all cell types including human primary fibroblasts. Knockdown of DHX9 leads to premature senescence. DHX9 has a helicase-like domain, as well as a bidirectional nuclear transport domain, that shuttles between the cytoplasm and nucleus.

The DHX9 protein is transported to the cell's surface and cytoplasm , where it binds to aptamer S-1. The protein is located on the 1q25 chromosome, which is a key locus for prostate cancer susceptibility. However, its relationship with colon cancer is unclear. We don't have a lot of information about the mechanism behind this marker. Its low-level of toxicity and high affinity make it an effective tool in tumor imaging.

The DHX9 gene is strongly associated with the genesis of DNA replication. Therefore, DHX9 suppression can result in cell cycle progress and DNA replication to be hindered. It also inhibits transcription. In the end, DHX9 plays a pivotal role in ensuring stability of the genomic regions. The DHX9 gene is multifunctional protein that aids in the progression of cells and the process of senescence.