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DGCR8 Antibodies
AND DGCR8 ELISA kits, DGCR8 Proteins
Many biological assays use antibodies to detect DGCR8, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DGCR8 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DGCR8 antibodies...
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DGCR8 Infographic by Boster Bio
All DGCR8 Products
Product
Figures
Specificity
Applications
Price
Human, Mouse
WB
$481
Cat # P00475
100 µl
Cat # P00475
100 µl
Human, Mouse, Rat
IP, IF, ICC, WB
$370
Cat # M00475
100 μl
Cat # M00475
100 μl
Human, Mouse, Rat
ELISA, IF, IHC, ICC, WB
$370
Cat # A00475-1
100 μg/vial
Cat # A00475-1
100 μg/vial
DGCR8 Overview
Facts about Microprocessor complex subunit DGCR8.
Microprocessor complex subunit DGCR8 (DGCR8)
Part of the microprocessor complex that acts as a RNA- and heme-binding protein that's involved in the first step of microRNA (miRNA) biogenesis. Part of the microprocessor complex that's required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus.
Within the microprocessor complex, DGCR8 be the molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs (PubMed:26027739, PubMed:26748718). The heme-bound DGCR8 dimer binds pri-miRNAs as a combined trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active.
Gene Information
| Human | |
|---|---|
| Gene Name: | DGCR8 |
| Uniprot: | Q8WYQ5 |
| Entrez: | 54487 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
C22orf12; DGCRK6chromosome 22 open reading frame 12; DiGeorge syndrome critical region 8; DiGeorge syndrome critical region gene 8; Gy1; microprocessor complex subunit DGCR8; pasha
Molecular Weight
Mass (kDA):
86.045 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 22q11.21 |
| Sequence: | 22; NC_000022.11 (20080232..20111877) |
Tissue Specificity
Ubiquitously expressed.
Subcellular Localizations
Nucleus. Nucleus, nucleolus. Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies (PubMed:17159994).
Diseases
- Malignant Neoplasms
- Digeorge Syndrome
- Neoplasms
- Schizophrenia
- Carcinoma
- Cell Transformation, Neoplastic
- Virus Diseases
- Melanoma
- Neoplasm Metastasis
- Malignant Squamous Cell Neoplasm
- Sarcoma
- Liver Carcinoma
- Hepatitis
Interacting Proteins
- DROSHA
- MEOX2
- SRPK1
Pathways
- Rna Interference
- Cell Proliferation
- Gene Silencing
- Translation
- Cell Cycle
- Pathogenesis
- Localization
- Rna Processing
- Aging
- Neurogenesis
- Skin Development
- Cell Differentiation
- Regeneration
PTMs
- Cleavage
- Reduction
Research Areas
- Epigenetics
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DGCR8
bosterbio.com/bosterbio-gene-info-cards/DGCR8
References
PMID: 12705904 by Shiohama A., et al. Molecular cloning and expression analysis of a novel gene DGCR8 located in the DiGeorge syndrome chromosomal region.
PMID: 15589161 by Landthaler M., et al. The human DiGeorge syndrome critical region gene 8 and its D. melanogaster homolog are required for miRNA biogenesis.