Dopamine beta-hydroxylase Antibodies

Dopamine beta-hydroxylase (DBH)

Conversion of dopamine to noradrenaline. .

Gene Information

Human
Gene Name:	DBH
Uniprot:	P09172
Entrez:	1621

Family

Belongs to:
copper type II ascorbate-dependent monooxygenase family

Alternative Names

DBH; DBM; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine betaHydroxylase; Dopamine beta-Hydroxylase; Dopamine beta-monooxygenase; DOPBHY; EC 1.14.17.1

Molecular Weight

Mass (kDA):
69.065 kDA

Genomic Context

Human
Location:	9q34.2
Sequence:	9; NC_000009.12 (133636363..133659329)

Subcellular Localizations

[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted.; Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein.

Diseases

• Nervousness
• Disease Of Adrenal Medulla
• Hypertensive Disease
• Depressive Disorder
• Schizophrenia
• Attention Deficit Hyperactivity Disorder
• Varicosity
• Pheochromocytoma
• Malnutrition
• Physiological Stress
• Neoplasms
• Neuroblastoma
• Hypotension Adverse Event

• Parkinson Disease
• Bipolar Disorder
• Psychotic Disorders
• Stress, Psychological
• Mental Disorders
• Pituitary Diseases
• Essential Hypertension

Pathways

• Innervation
• Localization
• Secretion
• Transport
• Pathogenesis
• Excretion
• Exocytosis
• Regeneration
• Aging
• Catecholamine Secretion
• Translation
• Reflex
• Neurogenesis

• Transpiration
• Glycosylation
• Sensitization
• Vasoconstriction
• Regulation Of Blood Pressure
• Regulation Of Gene Expression
• Cell Death

PTMs

• Phosphorylation
• Hydroxylation
• Cleavage
• Reduction
• Glycosylation
• Oxidation
• Amidation

• Deamination
• Decarboxylation
• Dephosphorylation
• Biotinylation
• Deiodination
• Acetylation
• Methylation

• Deglycosylation

Research Areas

• Cancer
• Lipid and Metabolism
• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DBH

Best Uses Of The DBH Marker

For scientists using the DBH marker is a useful instrument to determine the amount of DBH in different tissues. Scientists can present their results for different species, applications and special samples. They may also be eligible for product credits. The best part is that these results can be submitted by scientists from all over the world. There is no limit on the number of results one can submit. It doesn't matter if the scientist is located in the US, Europe, or Asia.

Anti-Dopamine beta Hydroxylase/DBH antibody

The Boster Bio Anti-Dopamine beta Hydroxylase (DBH) antibody is a polyclonal antibody that reacts with human, mouse and the rat DBH. The antibody is part of the Picoband(tm) antibody library. Boster Bio's validated validation process ensures its high affinity. Its affinity for DBH is higher than 99 percent in both Human and mouse.

The mRNA level of Th, Dbh, and Pnmt was detected by real-time PCR. Western blotting was used to measure the protein levels. Western Blotting was used to determine the levels of DA, DBH, and CRF in the plasma of porcine. The results were then analyzed using t-test. In a subsequent study, miR 375 was found to increase the expression of DA and DBH by up to 30 percent.

Previously, Boster Bio has produced an anti-Dopamine beta Hydroxylase antibody that recognizes DBH protein in a variety of samples. The antibody was tested on mice and human beings suffering from a variety of psychiatric conditions such as schizophrenia, schizoaffective disorder depression anxiety, depression, and Parkinson's disease. The company also makes a variety DBH antibodies that can be used in variety of research projects.

High-affinity primary antibody

Boster Bio High-affinity Primary Antibody is an immunoglobulin that is specifically designed to attract a specific antigen. Specificity and affinity determines the quality of an antibody. The affinity of the antibody is greater, and the antibody will not bind to unintended antigens. High-affinity primary antibodies are capable of detecting, purifying and measure the antigen that is targeted.

Boster Bio has over 1,000 ELISA kits available for all your high-affinity antibody needs. These kits are extremely sensitive and specific, long-lasting and thoroughly validated. Boster Bio Picokine(tm) ELISA kits, formulated with boster high-affinity primary antibodies, are used by more than 14,000 scientists. They are designed to detect the native forms of proteins that are present, these ELISA kits are sensitive to subpicogram and picogram levels of sensitivity.

To ensure that this reagent is suitable for the intended use, it should be confirmed by the International Working Group for Antibody Validation (IWG). Orthogonal analysis is a method to determine the presence of antibodies. This assures that the antibodies won't cause a signal when they are tested using knockouts for the target protein. The antibody must be tested for specificity by generation of antibodies to multiple epitopes of the same protein.

A high-affinity primary antibody is vital for sandwich ELISA. High-affinity antibodies can remove non-specific materials. This makes it a powerful instrument to test specific analytes in crude preparations. However, it can limit the dynamic range and sensitivity of the assay when used in the same way. Your research will be successful if choose the best primary antibody. You can anticipate the best results when you employ the right antibodies.

Detection of DBH in a variety of tissues

Detection of DBH in various tissues requires the use of a sensitive test. The precision of the test can be determined by analyzing the variance of DBH concentrations using duplicate analyses of three different types of tissue. Variability coefficients (CVs) of various tissues varied from 1% to 8 percent. Variability coefficients (CVs) of different tissues vary in iWAT, with it having a greater CV than the foliage.

The MBS2019107 kit, a microwell strip-plate ELISA for Dopamine Beta Hydroxylase (DbH), is ready-to-use. The test kit can detect native DbH and NE in the samples of different tissues. It also comes with quality control assays to guarantee the accuracy of the test.

CD86 can be detected in a variety tissues

Detection of CD86 in varying tissues has a variety of clinical and research applications. It has been discovered that the differing expression of CD86 in different types of PE cells could help in determining the prognosis of BC patients. It is believed that the molecule plays a crucial role in suppressing T-cell activation. Although the precise role of CD86 is still unclear, it is believed to have an important impact on the prognosis of those suffering from BC.

Studies on molecular biology have revealed CD86 expression on IPE cells is greater than that of RPE and CBPE cells. Therefore, it is likely that these IPE cells block T cell activation by a contact-dependent mechanism. Additionally, the presence CD86 in these tissues supports the notion that they inhibit T cells activation via a CD86-dependent mechanism.

Although further research is required, we already have some data to guide us in our search for a suitable imaging agent for CD80/CD86 within human tissue. 111In-DOTA-belatacept is a radiolabeled compound that accumulates in CD80/CD86-positive tissues. This molecule can be used as a benchmark for future imaging agents that target CD80/CD86.

It is important to know that anti-CD86 antibodies cannot completely reverse the suppressive effects of IPE. However anti-CD86 antibodies did help to lessen the suppression of IPE-activated cells, suggesting that IPE and CD86 directly interact with T cells. Anti-CD86 antibody is extremely specific for IPE and have a strong positive correlation with anti-CD86 capabilities.

Applications in pharmacology

Application of the DBH marker in pharmacological research is based on a high-performance liquid chromatography-fluorometric method, which allows for the measurement of DBH activity in plasma samples. This method is highly sensitive, allowing for the detection of tiny amounts of DBH product, even as low as one pmol. All measurements were performed in duplicate and average enzyme activities were reported in nanomoles/minute/ml plasma.

This type of study can be conducted using a variety analysis software. Some of these software include Cytoscape, Pajek, VisANT, GUESS, WIDAS, PATIKA, PATIKAweb, and CADLIVE. The DBH marker is also an excellent tool for analyzing network pharmacology. This marker is beneficial in pharmacology as many diseases are complex and can be caused by a variety of factors. Thus, a single medication is not likely to cure various diseases.