DAXX Antibodies

Death domain-associated protein 6 (DAXX)

Transcription corepressor proven to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription.

Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Appears to regulate transcription in PML/POD/ND10 atomic bodies together with PML and may affect TNFRSF6-dependent apoptosis thereby.

Gene Information

Human
Gene Name:	DAXX
Uniprot:	Q9UER7
Entrez:	1616

Family

Belongs to:
DAXX family

Alternative Names

BING2; CENP-C binding protein; DAP6; DAP6MGC126245; Daxx; death domain-associated protein 6; death-associated protein 6; death-domain associated protein; EAP1; ETS1-associated protein 1; Fas death domain-associated protein; Fas-binding protein; hDaxx; MGC126246

Molecular Weight

Mass (kDA):
81.373 kDA

Genomic Context

Human
Location:	6p21.32
Sequence:	6; NC_000006.12 (33318558..33323016, complement)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stre

Diseases

• Leukemia
• Acute Promyelocytic Leukemia
• Neoplasms
• Malignant Neoplasms
• Cytomegalovirus Infections
• Virus Diseases
• Malignant Paraganglionic Neoplasm
• Carcinoma
• Physiological Stress
• Cell Transformation, Neoplastic
• Prostatic Neoplasms
• Autoimmune Reaction

• Growth Arrest
• Myeloid Leukemia
• Ischemia
• Pancreatic Neoplasm
• Infective Disorder
• Mammary Neoplasms
• Neuroendocrine Tumors

Interacting Proteins

• AIRE
• AR
• ATRX
• CARD9
• CDCA7L

• CDKN2A
• CREBBP
• FAM9B
• FAS
• Fas

• FTH1
• GOLGA2
• H3F3A
• HDAC1
• HDAC2

• Hipk1
• HIST1H3A
• HIST2H3A
• HSPB1
• MAP3K5

• MCRS1
• MDM2
• NSD3
• PARK7
• Pax5

• PBXIP1
• PML
• PNMA1
• RASSF1
• RELA

• Ripk3
• SPOP
• SSX2IP
• STAT3
• SUMO1

• TCF7L2
• TGFBR2
• TP53
• TSG101
• UBC

• UBE2I
• USP7

Pathways

• Localization
• Cell Death
• Cell Cycle
• Pathogenesis
• Cell Proliferation
• Chromatin Remodeling
• Rna Interference
• Viral Replication
• Senescence
• Cell Growth
• Immune Response
• Mitosis
• Programmed Cell Death

• Methylation
• Nuclear Export
• Conjugation
• Cellular Localization
• Transport
• Induction Of Apoptosis
• Protein Sumoylation

PTMs

• Phosphorylation
• Sumoylation
• Acetylation
• Ubiquitination
• Methylation

• Cleavage
• Oxidation
• Deacetylation
• Reduction
• Dephosphorylation

Research Areas

• Apoptosis
• Cancer
• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DAXX

Best Uses Of The DAXX Marker From Boster Bio

Boster Bio's Anti-Daxx Antibody has many uses. But what is the most effective? This article will go over the advantages and the differences between each option. It will also explain what you can expect from an anti-daxx marker. Below are a few advantages. These are applicable to all scientists across the globe. In addition, you'll discover more about the Boster Bio Anti-Daxx antibody.

Boster Bio Anti-Daxx Antibody

Boster Bio Anti-Daxx Antibodies react to mouse, human, and rat sequences. They can be stored in a freezer for up to six months and are stable at temperatures of -20°C. The antibodies contain 5 mg BSA and 0.05 mg thimerosal. The DAXX marker is expressed as phospho-Syk.

Antibodies against phospho-Syk

Human Phosphosyk antibody identifies the phosphorylation at Y525/Y526, that is a DAXX markers for the phosphorylation. It is provided in a 0.2 um filtered solution in PBS that contains 0.09% sodium azide. It is then purified using a protein A column , and stored at 20°C. It is recommended to use small aliquots and avoid freezing and thawing cycles.

The role of Syk in the DAXX marker was examined by using a phosphorimager to detect and analyze phosphorylation Syk. Moreover, the anti-phospho-Syk monoclonal antibody AA4 resulted in a strong phosphorylation of Syk but only a weak signal for the release of histamine. Anti-Syk antibodies also block the activation and induction of cell death.

This study revealed that deficient syk cells in DT40 abrogated BCR-induced apoptosis, suggesting that constitutive Syk activity is essential for the process of apoptosis. Syk activity was not found to affect cell growth or increase the growth suppressive effects of anti-IgM. Furthermore, DT40 syk-deficient cells showed no differences in apoptosis, compared with controls.

Antibodies to the phosphogab2

PhosphoGab2 is a part of the Grb-associated binder (Gab) family of adaptor proteins that are recruited to the plasma membrane. Phosphorylation Tyr472 regulates the association of Gab1 and the p85-PI3 kinase. For binding to SHP2 the an phosphorylation process of Tyr627 or Tyr659 is essential.

Antibodies against phospho-PLCg2

Recent genome-wide associations studies have revealed links between rare codon variants in immune genes as well as the late-onset Alzheimer's disorder. One of these variants, a polymorphism in phospholipase Cgamma 2 (PLCG2) is protected against LOAD. PLCG2 is a critical component of signal transduction networks. This particular polymorphism (P522R) is believed to have an effect of protecting against LOAD.

This study has also shown that PLCg2 is highly enriched spatially within the microglia population. However, previously only mouse tissues was examined. The present study has clarified the local expression of phospho-PLCg2 the human brain. These findings have implications for AD research and management of the disease. It is possible that PLCg2 is involved in signaling pathways that stimulate TREM2 across a range of tissues including the brain.

PLCG2 is an phospholipase C family and is involved in transmembrane signaling. It is responsible for diacylglycerol, a secondary messenger, and inositol 1,4,5-triphosphate (IP3). There are four forms of PLCg2, PLCg2 or PLCg1. The four members of the group of the family, delta1 is the one that is the most prominent. It is found in the heart and lungs, as well as at higher levels in brain tissues.

Antibodies against phosphoryl-Akt

Boster Bio's anti-phospho-Akt antibody been tested in ELISA and Western blot on different types of cells. It binds to Zebrafish, Mouse, Rat, and Human. Numerous studies have proven its affinity to a high degree. Using this antibody will help you to conduct your research more efficiently. Its price is competitive too. Furthermore, it comes with a 100% money back guarantee.

The anti-phospho-Akt antibody came from rabbits that had been immunized with an KLH synthetic peptide conjugated to KLH. The antibodies were purified using affinity chromatography using an epitope specific phosphopeptide. The antibodies that are not phospho-specific were eliminated by chromatography. The antibodies for phospho-Akt are extremely specific and are available in various dilutions.

AGEs were used to determine the anti-AKT peptide antibody in HA-Akt cells. These cells were then left to starve for 16 hours before being stimulated with 1 mg/l insulin. The immunoprecipitated protein was then identified by Western blot. If the antibodies to phospho-Akt were shown to be inhibited by AGEs, HPA was the most important reason.

United States Biological is a prominent company in the field of life sciences. Its products include antibodies and recombinant proteins. The company also manufactures immunoassays. Its main focus is inborn immunity and inflammation as well as damage to cells due to pathogens. The company is dedicated to providing clinically relevant products and high-quality products for researchers. The company has more than three decades of experience marketing and producing antibodies and Recombinant proteins.

PP2A is a different Akt inhibitor. PP2A is a new signaling complex, and cAMP is vital for its function. Rap1b and Epac block Akt activity. PP2A is also required for Phosphorrylation dependent on cAMP. Therefore, PP2A inhibitors may inhibit Akt activity.