CD55 Antibodies

Complement decay-accelerating factor (CD55)

This protein acknowledges C4b and C3b fragments which float with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274).

Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents match damage (PubMed:28657829). .

Gene Information

Human
Gene Name:	CD55
Uniprot:	P08174
Entrez:	1604

Family

Belongs to:
receptors of complement activation (RCA) family

Alternative Names

CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD55; CR; CRdecay accelerating factor for complement (CD55, Cromer blood group system); CROMDAFcomplement decay-accelerating factor; DAF; decay accelerating factor for complement; TC

Molecular Weight

Mass (kDA):
41.4 kDA

Genomic Context

Human
Location:	1q32.2
Sequence:	1; NC_000001.11 (207321674..207360966)

Tissue Specificity

Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

Subcellular Localizations

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 7]: Cell membrane; Lipid-anchor, GPI-anchor.

Diseases

• Paroxysmal Nocturnal Hemoglobinuria
• Hemoglobinuria
• Hemoglobinuria, Paroxysmal
• Neoplasms
• Malignant Neoplasms
• Anemia
• Hemolysis (disorder)
• Inflammation
• Carcinoma
• Tissue Adhesions
• Aplastic Anemia
• Malignant Paraganglionic Neoplasm
• Leukemia

• Autoimmune Reaction
• Thrombosis
• Lymphoma
• Decreased Immunologic Activity [pe]
• Infective Disorder
• Anemia, Hemolytic
• Colorectal Cancer

Pathways

• Complement Activation
• Pathogenesis
• Cytolysis
• Localization
• Immune Response
• Cell Activation
• Secretion
• Complement-dependent Cytotoxicity
• Phagocytosis
• Inflammatory Response
• Cell Death
• Cell Adhesion
• Glycosylation

• Coagulation
• Tropism
• Cell Proliferation
• Regulation Of Complement Activation
• Transport
• Virulence
• Cell Cycle

PTMs

• Cleavage
• Glycosylation
• Gpi Anchoring
• Phosphorylation
• Acylation
• Biotinylation
• Dephosphorylation
• Reduction
• Deamination
• Methylation

• Geranylgeranylation
• Palmitoylation
• Deglycosylation
• Phosphorothioation
• Ubiquitination
• Iodination
• Deacetylation
• Acetylation
• Oxidation
• Myristoylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CD55

Best Uses Of The CD55 Marker From Boster Bio

In this article, we'll examine the primary antibodies available from Boster Bio. Boster's antibodies have been validated for immunohistochemistry and Western blotting and are trusted by the research community. These antibodies are made from recombinant proteins and polypeptides, and each represents an epitope of the antigen. In addition, Boster primary antibodies are highly specific and robust in a wide range of applications.

Boster's primary antibodies are trusted by the research community

Founded in 1993, Boster Biologicals is a life science research product supplier that offers primary antibodies, ELISA kits, and phospho-peptides. They are a well-respected name in the research community for their high-quality products that are validated on multiple matrices. All Boster products have undergone stringent quality control processes, and their primary antibodies are validated on multiple matrices.

Primary antibodies are used in many applications, including immunocytochemistry. Immunocytochemistry can be a powerful tool, and has been responsible for many breakthrough discoveries. However, some results from immunocytochemistry are inconsistent and may not be representative of the true extent of the disease. Sometimes investigators are unable to determine the problem, and further investigation has revealed that the results were incorrect. Then, they spend additional time on research, which may lead to the discovery that their primary antibody was not accurate.

CiteAb data is based on analyses of hundreds of thousands of antibody citations. This data can be used to gauge the market for research antibodies. It also reflects the reproducibility of the results. The scientific community has recently focused on reproducibility. Using CiteAb, Boster's primary antibodies are trusted by the research community. The company's quality initiative addresses this need. The company has received numerous awards and is committed to continuing to improve its quality and reliability.

To use primary antibodies, you must use a transfected cell line that expresses the antigen of interest. Then, fix the cells using the same methods as the experimental samples. These cells will have no unbound primary antibodies to act as a control. Therefore, a negative control is used when primary antibodies do not detect the protein in an experimental sample. Once the antibody binds to a tissue sample, it loses its function.

They are validated on Western Blotting, Immunohistochemistry and ELISA

The Boster Bio CD55 marker has been validated on Western Blotting, Immunochemistry and ELISA. The Boster CD55 antibody is a highly specific and highly sensitive tool that enables researchers to detect the expression levels of CD55 protein in tissue samples. The antibodies have been cited extensively in the scientific literature and have been validated on Western Blotting, Immunohistochemistry, and ELISA.

Western blotting is a commonly used method for protein detection. It involves the transfer of proteins from gel to membrane and subsequent selective immunodetection of immobilized antigen. Although Western blotting is a popular method for quantitative analysis of protein concentrations, it is limited by a large number of drawbacks, such as mediocre reproducibility and time-consuming manual operation. Moreover, it measures only one protein at a time and requires a large sample volume. Hence, it is not ideal for multiple protein measurements. Modern techniques for immunoblotting use miniaturized electrophoresis and capillary electrophoresis.

Whether you want to analyze a single sample or thousands of samples, an IHC test will be a valuable asset. Using Boster Bio's IHC antibodies can allow you to detect CD55 protein in any sample without the need for protein extraction or gel staining. In contrast to ELISA, IHC is a method that uses antibodies to detect a specific antigen in cells.

To validate Boster Bio CD55 markers on Western Blotting, Immunohistochemical and ELISA methods. This method requires the use of a suitable loading control antibody. It has been shown to detect lysozyme in human plasma at a concentration of 50 pg/mL. These results are highly relevant for the diagnosis and management of cancer patients.

The results of a Western blotting experiment were reported in a technical report. The results were compared across three methods. In Western blotting, proteins were extracted from the human breast cell line MDA-MB-231 and analyzed using various blotting membranes and visualization methods. The results showed that nitrocellulose and PVDF membranes provided clearer bands than the nylon and PVDF membranes. The PVDF membrane, however, gave clear bands on the back of the membrane and likely caused confusion.

They are optimized for Western Blotting

Optimizing your experiments is a vital part of achieving high-quality results. Luckily, Boster Bio offers many optimization guides and tips. These guides will help you determine how to optimize your experiments and get the best results possible. One of the most critical parts of sample preparation is choosing the right protein extraction method. A specialized extraction kit from Boster can make the difference between a blank blot and a beautiful blot. We recommend using the Boster guide to determine the correct extraction method for your experiments.

The primary antibodies used in western blotting are of high affinity and have been validated on a variety of methods, including ELISA and Western Blotting. The ELISA method is preferred by many researchers because it allows for single-isotype detection. The fluorescent secondary antibodies used in this method extend the dynamic range of quantitation, improve the correlation between the quantity of protein, and improve the overall quality of results.

Using the Boster Bio CD55 markers in your experiment is the easiest way to ensure quality data. These antibodies have been optimized for Western Blotting with the highest sensitivity possible. They are compatible with a wide variety of reagents and are compatible with all types of membranes. While using Boster Bio CD55 antibodies, you need to avoid common errors associated with Western Blotting to ensure reproducible results.

The primers for western blotting are designed for faster and slower protein transfer. The results are obtained by comparing the bands of proteins that were transferred with the four different blotting membranes. The proteins were analyzed with the help of two different visualization methods: nitrocellulose membrane and PVDF membrane. The nitrocellulose membrane presented more distinct bands than the other two. The PVDF membrane showed clear bands on the back of the membrane, which is probably what caused the confusion.

CD59 is a membrane complement regulatory protein. High levels of CD59 expression inhibit the formation of the complement attack complex and therefore cause the immune system to escape tumor cells. A previous study identified a peptide that binds to CD59 molecules. The resulting complex results in the targeted delivery of special drugs into tumor cells. You can even use CD55 markers in immunodiagnostic procedures as an adjunct to the CD55 antibody.

They are optimized for Immunohistochemistry

Optimum performance is crucial for any project, and Boster Bio CD55 markers are the key to achieving that goal. Boster's patented technology, based on the unique interaction of avidins and signal molecules, allows researchers to easily locate their protein of interest while ensuring high sensitivity and specificity. The company offers comprehensive technical resources, including blogs and disease information, as well as web-based digital tools. Additionally, Boster offers high-quality lysis buffers, which ensure good results and minimize cross-linking intensity.

For optimal results, sample preparation is crucial. Boster Bio has optimized its CD55 markers for immunohistochemistry by offering detailed protocols that walk you through the entire process step by step. The guide includes information on how to fix, embed, and slice samples for optimal results. It also provides troubleshooting tips. In addition to detailed information on sample preparation, Boster also offers comprehensive technical resources, including blogs and disease-specific disease information.