Caspase-4 Antibodies

Caspase-4 (CASP4)

Inflammatory caspase (PubMed:7797510, PubMed:23516580, PubMed:25119034). Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in reaction to non-canonical inflammasome activators (PubMed:24879791, PubMed:25964352).

Plays a vital role in the limitation of Salmonella typhimurium replication in colonic epithelial cells during infection (PubMed:25121752). In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately leads to pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion.

Gene Information

Human
Gene Name:	CASP4
Uniprot:	P49662
Entrez:	837

Family

Belongs to:
peptidase C14A family

Alternative Names

apoptotic cysteine protease Mih1/TX; CASP4; CASP-4; caspase 4, apoptosis-related cysteine peptidase; caspase 4, apoptosis-related cysteine protease; Caspase4; Caspase-4; EC 3.4.22; EC 3.4.22.57; ICE(rel)II; ICE(rel)-II; ICEREL-II; ICH2; ICH-2; Mih1/TX; Protease ICH-2; Protease TX; TX

Molecular Weight

Mass (kDA):
43.262 kDA

Genomic Context

Human
Location:	11q22.3
Sequence:	11; NC_000011.10 (104942866..104968596, complement)

Tissue Specificity

Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.

Subcellular Localizations

Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted. Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630).

Diseases

• Malignant Neoplasms
• Neoplasms
• Endoplasmic Reticulum Stress
• Carcinoma
• Physiological Stress
• Inflammation
• Malignant Tumor Of Colon
• Blind Vision
• Leukemia
• Malignant Paraganglionic Neoplasm
• Alzheimer's Disease
• Cell Transformation, Neoplastic
• Melanoma

• Malignant Neoplasm Of Breast
• Mammary Neoplasms
• Malignant Squamous Cell Neoplasm
• Ovarian Neoplasm
• Malignant Neoplasm Of Lung
• Malignant Neoplasm Of Ovary
• Neuroblastoma

Pathways

• Cell Death
• Pathogenesis
• Induction Of Apoptosis
• Translation
• Cell Cycle
• Cell Proliferation
• Programmed Cell Death
• Immune Response
• Proteolysis
• Inflammatory Response
• Sensitization
• Protein Folding
• Rna Interference

• Localization
• Drug Resistance
• Antigen Processing And Presentation
• Cell Growth
• Angiogenesis
• Autophagy
• Secretion

PTMs

• Cleavage
• Phosphorylation
• Glycosylation

• Dephosphorylation
• Deglycosylation
• Ubiquitination

Research Areas

• Apoptosis
• Cancer
• Caspases

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CASP4

Boster Bio - Best Uses Of The CASP4 Marker

You have come to the right spot if you are looking for an affordable marker anti-caspase 4 Boster Bio’s Anti-Caspase 4/CASP4 marker is a perfect addition to the Picokine ELISA platform. Continue reading to learn more about the CASP4 markers benefits and features. It's a revolutionary new tool for ELISA researchers, and it's applicable to scientists world-wide.

Boster Bio's Anti-Caspase 4/CASP4 Marker

The Anti-Caspase 4/caspase-4 marker was tested by WB on human. The product contains five micrograms of BSA, 0.05mg Thimerosal, which reacts to the caspase 4 gene. The resulting protein can be expressed in one cytosolic DNA chromosome. The anti-Caspase 4 antibody was created by Boster Bio and is a product of the Bio-Caspase-Caspase-Caspases research community.

CASP4 belongs to the caspase group and has been linked with the development more effective anticancer therapies. CASPs are targeted by anticancer drugs that act upstream of their substrates. Although CASP4 has not been linked to a prognostic significance in ccRCC, high levels of CASP4 mRNA were associated with a worse overall survival (OS) than patients with a normal CASP4 expression level.

CASP4 expression is also implicated in the tumor cell's sensitivity to cancer drugs. This marker may be a potential therapeutic target in cancer treatments for increasing drug sensitivity or overcoming drug resistance. Researchers now have the Anti-Caspase 4/CASP4 marker available for clinical trials.

Boster Bio's Anti-Caspases 4/CASP4 antibody marker is clinically useful for the diagnosis and treatment of cancer patients. The antibody is compatible with both serological and immunohistochemical methods. Anti-Caspase 4/CASP4 marker (BAC), detects CASP4 expression in high levels of sensitivity. This antibody is a new immunohistochemical antigen to help patients with lung cancer.

Poor prognosis is associated to high levels of CASP4 in renal cells. Data from the Cancer Genome Atlas and Oncomine databases were used to evaluate the expression levels of CASP4 in ccRCC patients. The data were also used in identifying pathways and determining CASP4's role in ccRCC. The 95% confidence range is represented by the shaded region.

Boster Bio's Picokine ELISA Platform

Picokine(tm), an ELISA platform that measures protein and cytokine levels at picogram-level sensitivity allows researchers to measure a protein in just minutes. The kits can be used to validate a variety if samples. The validation procedures and images are available upon request. The Picoband platform is powered by insights into immunogen design and technical support from BeNeLux distributor Sanbio.

Sword ELISA Booster works in any ELISA to improve sensitivity. It's a proprietary technology, which interacts with the streptavidin and detection antibody. Once it is exposed to a light photon, it becomes a Raman active molecule. Raman-active molecules refract a second photon, which results in a strong signal derived at a low HRP level. This patented technology improves sensitivity as well as precision at the low end ELISA spectrum.

Benefits of using CASP4 Marker

CASP4 expression reduces the survival and spread of B. Cenocepacia infected individuals. This marker is also associated to increased inflammatory cytokine and biomarker MPO production. It also decreases survival of the host. These are just a couple of the benefits of the CASP4Marker. These benefits should help researchers better understand CASP4’s role in the immune system's response to infection.

The CASP4 gene plays a role in the regulation of innate immunity as well as T cell immune responses. The activity of CASP4 modulates actin polymerization in L. pneumophila-infected macrophages, which aids in the transport of phagosomes to lysosomes. CASP4 activity in the trafficking and transport of phagosomes to lysosomes is significantly lower than that of nonpathogenic Escherichia.