This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Cytokine receptor common subunit beta.
Human | |
---|---|
Gene Name: | CSF2RB |
Uniprot: | P32927 |
Entrez: | 1439 |
Belongs to: |
---|
type I cytokine receptor family |
beta, low-affinity; CD131 antigen; CD131; CDw131; colony stimulating factor 2 receptor, beta, low-affinity(granulocyte-macrophage); Common beta Chain; CSF2RB; cytokine receptor common subunit beta; GM-CSF R beta; GM-CSF/IL-3/IL-5 receptor common beta subunit; GM-CSF/IL-3/IL-5 receptor common beta-chain; IL-3 R beta; IL3RB; IL-5 R beta; interleukin 3 receptor/granulocyte-macrophage colony stimulating factor 3receptor, beta (high affinity)
Mass (kDA):
97.336 kDA
Human | |
---|---|
Location: | 22q12.3 |
Sequence: | 22; NC_000022.11 (36913574..36940449) |
Membrane; Single-pass type I membrane protein.
Consider the quality of the primary antibodies if you are looking for a Human GM–CSF ELISA Kit. Boster offers high-affinity antibodies that have been extensively cited in the research community. They are backed by a range of validation methods, including Western Blotting, Immunohistochemistry, and ELISA. The CSF2RB biomarker is a well-established marker in the central nerve system.
One of the best ways to analyze CSF2 RNA is through an ELISA. It is a highly sensitive test that is available for a wide variety of samples. Boster Bio ELISA kits have been validated for specific samples. They are available in a wide range of formats, including ELISA, Western Blotting, Immunohistochemistry, and more.
The Human GM–CSF ELISA Kit, a solid-phase ELISA that measures levels of recombinant GM–CSF (serum or plasma), is a solid phase ELISA. It contains recombinant GM–CSF from humans and antibodies that were raised against it. The results show linear curves. The kit is suitable for diagnostic and research purposes. You can order a Human GM–CSF ELISA Kit through our online shop.
The Human GM–CSF ELISA ELISA Flex kit is ideal if you prefer a flexible ELISA setup. It includes a matched pair if capture and detection antibodies, as well as a standard reconstitution buffer. This kit is ideal if your goal is to test human GM–CSF using a small sample. It also contains Streptavidin HRP and biotin conjugated anti.
Human GM-CSF ELISA kits uses a sandwich ELISA approach to detect endogenous GMCSF. A micro ELISA plate is coated first with a monoclonal antibody against GM-CSF. Next, standards and test samples are added. The second antibody is then biotin-conjugated with a goat anti-Biotin-Peroxidase (HRP). After washing the microplate, use TBS buffer or PBS buffer to remove any unbound conjugates. Finally, wash the microplate with a stop solution. Finally, after the kit has developed, it will display a yellow hue.
The Human GM–CSF genes are located on the human chromosomes 5q21-5q32. They have been implicated in many diseases, including 5q Syndrome and acute myelogenous lymphoma. A complementary DNA cloned by GM–CSF was isolated in yeast and purified. The purified protein is capable of stimulating peripheral blood monocytes.
The Human GM–CSF ELISA Kit contains a reagent mixture, pre-coated strips plates, a secondary antibodies, and buffers to detect GM–CSF in biological samples. The kit also includes instructions for performing the test and storage guidelines. Although the assay can be completed in three hours, it is possible to obtain quantitative results in as little time as three days.
The Human GM–CSF ELISA Kit can be used to determine the levels in serum, cell culture supernatants, plasma and other fluids. It should be used for research purposes only and should not be used for diagnostic purposes. If you are using it to conduct clinical research, please consult your physician. Order your Human GM–CSF ELISA Kit online.
The Human GM–CSF ELISA Kit for Blood is a simple and easy-to-use test to detect GM–CSF in blood samples. GM-CSF is a glycoprotein that induces the proliferation of myeloid progenitor cells and differentiation of mature neutrophils and eosinophils. CSF2 encodes the human GM–CSF protein gene. The human gene shares approximately 65% identity to the mouse version and 63 percent with the canine counterpart.
Spectrophotometry is used to determine optical density for the Human GM–CSF. The wavelength at which the light reaches the sample is 450nm. The concentration of GM-CSF can then be calculated by comparing absorbance of the unknown sample with the standard curve. This value is multiplied then by the dilution number.
PMID: 1702217 by Hayashida K., et al. Molecular cloning of a second subunit of the receptor for human granulocyte-macrophage colony-stimulating factor (GM-CSF): reconstitution of a high-affinity GM-CSF receptor.
PMID: 17828305 by Kao C.J., et al. CBAP interacts with the un-liganded common beta-subunit of the GM- CSF/IL-3/IL-5 receptor and induces apoptosis via mitochondrial dysfunction.