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15 Citations 2 Q&As
Facts about Collagen alpha-2(IV) chain.
Human | |
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Gene Name: | COL4A2 |
Uniprot: | P08572 |
Entrez: | 1284 |
Belongs to: |
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type IV collagen family |
Collagen alpha-2(IV) chain
Mass (kDA):
167.553 kDA
Human | |
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Location: | 13q34 |
Sequence: | 13; NC_000013.11 (110307284..110513209) |
Secreted, extracellular space, extracellular matrix, basement membrane.
If you're looking to find canstatin in various cell types You can purchase boster bio's COL4A2 marker. This marker can be used in vitro and is suitable for a variety of different applications. Learn more about the advantages and requirements of a boster COL4A2 marker and how to use it in your next research project.
Recombinant canstatin, a type IV collagen molecule that has an NC1 domain, is an effective inhibitor of lymphangiogenesis as well as angiogenesis. It hinders the growth of CT-26 cells under hypoxia-stimulated conditions and also blocks migration and tube formation in HUVEC and lymphatic cells treated with rhAngpt-1. Canstatin also inhibits the proliferation of HEK-293 cells.
Recombinant canstatin hinders cell migration and LEC tube formation by diminuting HUVEC migration by 47.9% and 55% respectively. The inhibitor did not have any effect on LEC proliferation or basal tube formation. However, it decreased LEC activity in migration and reduced the amount of HUVEC. This suggests that canstatin may inhibit the proliferation of certain cancerous cells. The results of this study are not conclusive.
Recombinant Canstatin blocks colon cancer cell growth in mice , and in vitro. It also inhibits VEGFR-3 as well as Tie-2, which are key cancer-promoting proteins. Canstatin is also thought to suppress cell death by inhibiting the activity of integrin-dependent FAK, a family of proteins that are vital for the development of cancer. This suggests that canstatin may be beneficial in a range of applications that include CNV and cancer.
Canstatin is not only a deterrent to the growth of tumors, but also blocks the development of new tumors. Canstatin blocks angiogenesis associated with tumors by binding to endothelial cells. In vitro, it induces apoptosis in some tumor cells. This effect is mediated by the process of apoptosis. This is crucial for cancer patients. However clinical trials are required to verify the efficacy of canstatin.
In addition to suppressing the proliferation and promoting a decrease in phosphorylation of FAK in HUVEC. LEC is more affected by canstatin, which inhibits FAK phosphorylation through rhAngpt-1 at 40 mg/ml. This suggests that canstatin can target both preformed and angiogenic cells.
The affinity chromatography method was employed to isolate the recombinant protein from 293 cells. Immunoblot analysis revealed that the monomer was present in the main peak. The molecule binds to a monomer approximately 24 kDa by using canstatin antibodies. Further purification of the recombinant protein is done. These studies have uncovered the unique mechanism of action for canstatin.
Boster's COL4AA2 marker offers many advantages beyond the fact that it detects the protein with high accuracy. This marker also permits scientists to report results for species-specific or applications-specific samples, as being able to receive product credits for their results. These advantages are available to all scientists across the globe. These benefits allow scientists to conduct their research more efficiently.
Nordic Bioscience ELISA is designed to detect canstatin. It detects COL4A1/A2-mediated degradation in a variety cell types. Using an ELISA kit biotinylated synthetic propeptides were added to 96-well plates and washed five time with 20 mmol/L Tris/50 mmol/L NaCl pH and the standard peptide was then added to each well.
COL4A2 is a marker that can be found in ECM of PDLCs or BMCs. It binds bone morphogenetic and angiogenic proteins, presenting them in osteoprogenitor-like cells and triggering osteogenesis. COL4A2 is found in both PDLCs as well as BMCs. It plays a role in the osteogenic development and development of PDLSCs.
Type IV collagen A2 is a part of the structural structure of the basement membrane. It forms a heterotrimer along with its COL4A1 counterpart COL4A1 to participate in a variety of dynamic biological processes. Its expression in cancer cells can cause cerebral hemorrhage as well as small vessels disease. It helps to promote the differentiation of embryonic stem cells into endothelial cell types.
PMID: 3198637 by Hostikka S.L., et al. The complete primary structure of the alpha 2 chain of human type IV collagen and comparison with the alpha 1(IV) chain.
PMID: 3345760 by Brazel D., et al. Human basement membrane collagen (type IV). The amino acid sequence of the alpha 2(IV) chain and its comparison with the alpha 1(IV) chain reveals deletions in the alpha 1(IV) chain.
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