Centromere protein H Antibodies

Centromere protein H (Cenph)

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and can be involved in incorporation of newly synthesized CENPA to centromeres.

Required for chromosome congression and efficiently align the chromosomes on a metaphase plate (By similarity). .

Gene Information

Mouse
Gene Name:	Cenph
Uniprot:	Q9QYM8
Entrez:	26886

Family

Belongs to:
CENP-H/MCM16 family

Alternative Names

CENP-H; centromere protein H; ICEN35; Interphase centromere complex protein 35; kinetochore protein CENP-H; NNF1; PMF1

Molecular Weight

Mass (kDA):
28.135 kDA

Genomic Context

Mouse
Location:	13 53.23 cM|13 D1
Sequence:	13;

Tissue Specificity

Abundantly expressed in thymus, spleen, uterus, ovary, testis and muscle, and weakly expressed in small intestine, lung and stomach. Barely detectable expression in kidney, liver, skin and prostate gland. Not detected in brain, heart or adrenal gland. Also expressed weakly in various hematopoietic cell lines.

Diseases

• Malignant Neoplasms
• Carcinoma
• Neoplasms
• Aneuploidy
• Malignant Paraganglionic Neoplasm
• Cancer Of Nasopharynx
• Colorectal Neoplasms
• Colorectal Cancer
• Malignant Squamous Cell Neoplasm
• Nasopharyngeal Neoplasms
• Stomach Neoplasms
• Sclerosis

• Neoplasm Metastasis
• Mutation, Out-of-frame
• Malignant Neoplasm Of Tongue
• Malignant Neoplasm Of Breast
• Refractory Cytopenia With Multilineage Dysplasia
• Diffuse Scleroderma

Pathways

• Mitosis
• Localization
• Chromosome Segregation
• Cell Cycle
• Metaphase
• Interphase
• Rna Interference
• Cell Division
• Cell Proliferation
• Kinetochore Assembly
• Response To Paclitaxel
• Cell Cycle Arrest
• Cytokinesis

• Chromatin Organization
• Spindle Assembly Checkpoint
• Spindle Assembly
• Chromatin Remodeling
• G2 Phase
• Dna Methylation
• Methylation

PTMs

• Acetylation

• Methylation

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Cenph

Boster Bio - Best Uses Of The CENPH Marker

Boster Bio's high affinity and specificity may be something you are considering if you're using primary antibodies in your research. This article will provide information on why high-affinity antibodies are important and the benefits of the CENPHMarker. We will also discuss how the CENPH Marker can be used to increase the power of your research.

Boster Bio has high-affinity, primary antibodies

Flow cytometry can be used to measure the activity of cells and particles in a variety of applications. Boster Bio supplies high-affinity, primary antibodies against CENPH marker. These have been validated for use across a range of applications. Their proprietary ELISA platform uses proprietary trade secrets to deliver highly sensitive ELISA kits. Boster Bio can provide an antibody against CENPH for tissue-based or cell-based analysis.

Primary antibodies are immunoglobulins that bind to the antigen in question. Their quality is determined primarily by their affinity or specificity. This refers to the strength of non-covalent bonds between the antibody (and the antigen). A high-affinity primary antibody will recognize the antigen of interest and bind to it with a low amount of unintended binding. High-affinity primary antibodies provide the best and most reliable way to detect, purify and measure the CENPH marker.

Boster Bio provides antibodies that recognize CENPH markers, but also antisense clones. These clones can be used in a variety if applications. The CENPH marker was found in catfish, which is a model organism for autoimmune disorders. A human immune response to AIDS-related antigens can be triggered by affinity maturation in follicular dendritic cells.

A secondary antibody is used together with a primary to detect a particular antibody. Typically, these antibodies are used as core components in immunoassays. The ELISAs utilize antibodies to bind to antigens. Examples of immunoassays are Western Blotting, immunohistochemistry, and histopathology. Flowcytometry, however measures the presence in a certain cell type.

IgNAR is a novel antigen receptor with few loci with single VNAR-elements. Adult nurse sharks express two loci. An analysis of 51 zebrafish immunoglobulins by high-throughput sequencing showed that somatic mutations were more common with age than with previous exposure to disease. If you are interested in finding out if this antigen causes autoimmune reactions, high-affinity prima antibodies should be your best option.

Optimize your experiments by using primary antibodies

You need to select the right antibody for your experiments with primary antibodies. This will ensure that you optimize your experiments. To ensure the antibody is compatible with the target, you need to perform antibody validation. You will need to search for relevant research papers and published articles. However, you should be cautious while selecting antibodies, as inaccurate information has contributed to the current reproducibility crisis. Also, it is important to seek input from other researchers and colleagues.

The first step in hypothesis driven research is to get a rough idea of the function of your target protein within cells. After you have discussed your ideas with collaborators, you are ready to start designing your experiments. This process involves hypothesis refinement as well as experimental design. Using the CENPH marker as a reference may provide additional insight into the biology of the target. After determining the target protein, you can further optimize your experiments with the antibodies.

Secondary antibodies can also be conjugated to fluorescent substrates. It is important to choose an antibody that recognizes both your target protein and the fluorophore. For example, if your target protein is a bacterial bacterium, you should use an antibody conjugated to it. This is the best method to multiplex the experiment, and it also eliminates the risk of false positive results.

Benefits of using CENPH Marker

CENPH can be used to detect if a tumor is overexpressing CENPH. This gene is important in cell cycle regulation as well as survival and is expressed in many human tumors. Interestingly, there have been reports that overexpression of CENPH in human cancer cells is associated with poor prognosis. CENPH genes are found in all human tissues. CENPH elevation has been linked both to aneuploidy as well as poor prognosis in many solid tumors.