CDR2 Antibodies

Cerebellar degeneration-related protein 2 (CDR2)

Gene Information

Human
Gene Name:	CDR2
Uniprot:	Q01850
Entrez:	1039

Family

Belongs to:
CDR2 family

Alternative Names

CDR62; cerebellar degeneration-related protein (62kD); cerebellar degeneration-related protein 2; cerebellar degeneration-related protein 2, 62kDa; Major Yo paraneoplastic antigen; Paraneoplastic cerebellar degeneration-associated antigen; PCD17; Yo paraneoplastic antigen; Yo

Molecular Weight

Mass (kDA):
51.855 kDA

Genomic Context

Human
Location:	16p12.2
Sequence:	16; NC_000016.10 (22345936..22374619, complement)

Diseases

• Paraneoplastic Cerebellar Degeneration
• Malignant Neoplasms
• Neoplasms
• Autoimmune Reaction
• Paraneoplastic Syndromes
• Candidiasis
• Cerebellar Diseases
• Autoimmune Diseases
• Ovarian Neoplasm
• Carcinoma
• Hiv Infections
• Adenocarcinoma

• Lupus Erythematosus, Systemic
• Lymphoma
• Infective Disorder
• Nervousness
• Paraneoplastic Syndromes, Nervous System
• Mammary Neoplasms

Interacting Proteins

• ANKRD36BP1
• C1orf216
• C2CD6
• C4orf46
• CCDC153

• CDR2L
• CHIC2
• COX5B
• EIF4E2
• ENKD1

• FAM110A
• FAM161A
• HAUS1
• HDAC4
• HGS

• KANSL1
• KIFC3
• LENG1
• LIN37
• MORF4L2

• MRPL28
• PKN1
• RCOR3
• RSPH14
• SCNM1

• SH2D4A
• SMARCE1
• TBC1D22B
• TCHP
• TRIM23

• TTR
• TXLNA
• WDYHV1

Pathways

• Drug Resistance
• Immune Response
• Pathogenesis
• Gene Conversion
• Transport
• Cell Cycle
• Mitosis
• Glycosylation
• Localization
• Cell Adhesion
• Cell Activation
• Cytokinesis
• Cell Division

• Secretion
• Cell Recognition
• Cell Death
• T Cell Activation
• Reverse Transcription
• Interphase
• Hypersensitivity

PTMs

• Glycosylation
• Phosphorylation
• Cleavage
• Biotinylation

• Deamidation
• Hydroxylation
• Dephosphorylation
• Ubiquitination

Research Areas

• Cancer
• Immune System Diseases
• Immunology
• Neuroscience

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CDR2

Best Uses of the CDR2 Marker

For more information about Boster's background and the latest CDR2 protein markers, read our Bio: The History of the CDR2 Protein Marker. We also discuss how to make high-affinity prima antibodies and the best format for CDR3 inserts. In addition, we cover a few key techniques for detecting the CDR2 protein marker. Let's take an in-depth look at these three techniques.

History of Steven Boster

Steve Boster was a Joliet native, IL resident, who passed away in Madison, WI, on June 26, 2022. He was a former retail sales manager and a U.S. Army veteran. His parents were James Meier (and Evelyn Meier) and he was a member Concordia Hall, Staunton. Steve is survived by Nancy Boster, his wife and two daughters, Natosha and Crystal Peck, as well as 6 grandchildren, 4 brothers, Jack and David Boster, and Sandra Blanton, his sister. There are many nieces, nephews, and cousins.

Steve was devoted to his family above all. He was devoted to his family and loved his two sons above all else. He was the first person he called when his car broke down at 2 a.m., and he often showed up in sub-zero weather. His generosity extended to his friends as well. He treated them like family, and was always available for them. Steve was a champion at Chess and he continued to be competitive in the game of golf after learning about his mortality.

High-affinity primary antibodies

This method can increase the number high-affinity antibodies by using genetic engineering tools and phage-display technology. The CDR2 marker is a highly conserved region in the human antibody molecule. This mutation makes high-affinity antibodies easier to make in bacteria. The CDR2 marker is also highly conserved in mouse antibodies.

Toxin-conjugated antibodies, or Nbs, can work effectively in various biological applications. These fragments are able to be taken up by the targeted cell via endocytosis and killed. For example, scFv may be conjugated with bacterial toxin A or human cytotoxic elements such as granzyme B.

The first step to develop high-affinity antibodies was the design and expression of gene fusions. Next, they were separated and their affinities and Kinetic constants calculated. The authors were then able to calculate the affinities, kinetic constants and specificities of the antibodies. The CDR2-tagged antibodies were then tested on mice schizont cells.

Single-chain variable domains can be developed that retain the functional activity of parent Abs and maintain the affinity of parent Abs. The single variable domain was first discovered in cartilaginous fish. It was a breakthrough for single domain antibody research. These antibodies had low affinity, poor solubility and high production costs. Only a few single-chain variable domains for human use have been created.

AbD16714, an Anti-Trastuzumab Antibody, was affinity maturated by replacing its heavy chains CDR2 and using the library. This was achieved with conventional restriction cloning. CDR-diversified CDR librarians were generated using trinucleotide binding blocks and affinity maturity. The maturation library generated 313 anti–trastuzumab anti-trastuzumab proteins. 70 unique antibodies were discovered in sequences of 95 positive Clones.

The Nb lead gene must be tailored to specific applications. Genetic fusion of the lead Nb gene and another gene in a laboratory yields an exact construct. The domain's 5′ end is usually where the Nb gene for lead is kept. Once the DNA is fused together, it will form a Nb gene that binds Ag. These antibodies will be created through affinity maturation.

CDR3 Injections in their optimal format

Optimizing the CDR3 insertion format has been shown to improve the quality and yield of protein sequences. The process involves designing new CDRs based on a framework CDR1-CDR2 sequence. This method produces CDR sequences with a lower cosine to the nearest neighbour than the natural repertoire while still maintaining key statistical properties for variable-length sequences.

Detection M&R LE protein marker

M&RLE detection is a sensitive method that detects proteins having an M&R epitope embedded in their M&R like tails. M&RLE protein marker, which is typically between 15-120kDa in size, is formed through the fusion M2/R2 epitopes. This product is a combination of anti-rabbit and anti-mouse secondary antibodies that detect proteins. The M&R LE proteins marker has a high molecular-weight resolution and is an affordable tool for protein analysis.

This antibody allowed us to analyze CDR function in a more thorough way. The ELISA data was used to determine the protein's affinity coefficient. The affinity constant for the HCDR1–G9–L3–R2/K–LH antibody was 3.7 x 10 8 Liters/mol.