CDK5 Antibodies

Cyclin-dependent-like kinase 5 (CDK5)

Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by activating abortive cell cycle re-entry. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins.

Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and boosts CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates like Rho and Ras family small GTPases (e.

Gene Information

Human
Gene Name:	CDK5
Uniprot:	Q00535
Entrez:	1020

Family

Belongs to:
protein kinase superfamily

Alternative Names

CDK5; CDKN5; Cell division protein kinase 5; cyclin-dependent kinase 5; EC 2.7.11; EC 2.7.11.22; LIS7; protein kinase CDK5 splicing; PSSALRE; Serine/threonine-protein kinase PSSALRE; Tau protein kinase II catalytic subunit; TPKII catalytic subunit

Molecular Weight

Mass (kDA):
33.304 kDA

Genomic Context

Human
Location:	7q36.1
Sequence:	7; NC_000007.14 (151053815..151057897, complement)

Tissue Specificity

Isoform 1 is ubiquitously expressed. Accumulates in cortical neurons (at protein level). Isoform 2 has only been detected in testis, skeletal muscle, colon, bone marrow and ovary.

Subcellular Localizations

[Isoform 1]: Cytoplasm. Cell membrane; Peripheral membrane protein. Perikaryon. Cell projection, lamellipodium. Cell projection, growth cone. Cell junction, synapse, postsynaptic density. In axonal growth cone with extension to the peripheral lamellipodia (By similarity). Under neurotoxic stress and neuronal injury conditions, CDK5R (p35) is cleaved by calpain to generate CDK5R1 (p25) in response to increased intracellular calcium. The elevated level of p25, when in complex with CDK5, leads to its subcellular misallocation as well as its hyperactivation. Colocalizes with CTNND2 in the cell bod

Diseases

• Alzheimer's Disease
• Neurodegenerative Disorders
• Nerve Degeneration
• Nervousness
• Neurofibrillary Tangles
• Malignant Neoplasms
• Neoplasms
• Sclerosis
• Amyotrophic Lateral Sclerosis
• Neurotoxicity Syndromes
• Primary Lateral Sclerosis
• Parkinson Disease
• Neuroblastoma

• Tissue Adhesions
• Tauopathies
• Inflammation
• Retinoblastoma
• Diabetes Mellitus
• Nervous System Disorder
• Ischemia

Interacting Proteins

• CABLES1
• CCNC
• CCND2
• CCND3
• CDK5R1

• CDKN1A
• CDKN1B

Pathways

• Cell Cycle
• Hyperphosphorylation
• Cell Death
• Pathogenesis
• Localization
• Cell Proliferation
• Transport
• Brain Development
• Secretion
• Neuroprotection
• Cell Division
• Neurogenesis
• Proteolysis

• Cell Migration
• Cell Cycle Arrest
• Synaptic Transmission
• Protein Phosphorylation
• Endocytosis
• Cell Differentiation
• Exocytosis

PTMs

• Phosphorylation
• Cleavage
• Dephosphorylation
• Ubiquitination
• Acetylation
• Methylation
• Nitrosylation
• Myristoylation

• Ribosylation
• Biotinylation
• Sumoylation
• Amidation
• Nitration
• Glycosylation
• Amination
• Oxidation

• Deglycosylation

Research Areas

• Cell Cycle and Replication
• Innate Immunity
• Phospho-Specific

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CDK5

Boster Bio - Best Uses Of The CDK5 Marker

This article will review the Boster Bio Anti CDK5 antibody. This article will describe the capabilities of the antibody for your research. It reacts with human Cyclin dependent kinase 4(CDK4) and is able to store at -20degC up to one year. The Immunofluorescence confirmed this antibody. It is available to purchase directly from Boster Bio, catalog # A00159-1.

Boster Bio Anti-CDK5 antibody

Boster Bio offers an anti-CDK5 antibody that is highly effective in IHC and ELISA applications. This antibody has been tested for specificity and is able to react with Rat, Mouse, and Human. It is available in PBS containing 0.09 percent sodium azide. It is made by rabbits that have been immunized with synthetic progesterone. It interacts with the CDK5 protein within cells, tissue, and other tissues.

In in vitro, this antibody detects phosphorylation of PIPKIg90 at Ser453. Human cells were co-transfected FLAG-PIPKIg90 and the protein was then immunoprecipitated using anti-FLAG agarose beads. In vitro, the anti-PIPKIg (pS453) antibody detects PIPKIg90 phosphorylation at Ser453. It is important to know that HGF and EGF are known to trigger the phosphorylation PIPKIg90 at Ser453 and Thr553, respectively.

Boster Bio Anti-Cyclin-dependent-like kinase 4 Cdk4 Marker

This antibody reacts with Human Cyclin-dependent Knase 4 (Cdk4) that is an important transcription factor in the progression of cells. It is stored at -20degC and can be used for a period of one year. This antibody has been confirmed by immunofluorescence and known positive and negative samples. It is therefore a good option for research into cell division.

CDK4 is an enzyme of the protein-serine kinase that regulates cell cycle in human cells. It is involved in controlling cell proliferation during the G1 phase. It is part of a group of proteins known as cyclin dependent kinases. In the process of cell division, CDK4 and D-type cyclins form complexes. Cell proliferation during the G1 phase is affected by Cdk4 Cyclins and D-type Cyclins. In addition, it is connected with p16 which is a cyclindependent inhibitor of kinase-2.

In vitro studies have shown that Cdk4 is involved in numerous malignancies, including melanoma, but not only. It is responsible for preventing cyclin D1 binding to CDK4/6, and inducing G1 cell cycle to stop. It also plays a role in triggering cellular senescence, an event in which cells lose their proliferative capabilities. Its absence has been linked to the development of hypoplasia, diabetes and pituitary dysfunction.

To determine CDK4, reverse transcription of breast cancer cells that express the 67NR mCDK4 gave three bands on agarose gel. Two bands included wt CDK4 and the third was a variant lacking the entire 234-bp extension 2. AceView assigned the bands c and B to the mCDK4–d and wt/DE2 heterodimers.

This antibody was produced against hCDK4 protein (wt CDK4). It showed a lower affinity to human proteins , however it showed higher affinity to smaller isoforms. It recognized multiple HA-tagged proteins , in addition to CCND1 antibody and heavy-chain mouse immuneglobulin, which is lower than the wt CDK4 antibodies.

Part 1 of this research designed to identify biomarkers that might enrich the patient population responsive to CDK4/6 inhibition. Part 2 was designed to determine whether the presence of these biomarkers could aid in the treatment of cancer patients. The results of this study suggest that the presence of these biomarkers will increase the chances of a successful treatment.

Boster Bio Anti-Cyclin-dependent-like kinase 5 Cdk5 Marker

The Boster Bio Anti-Cyclin Depend-Like Kinase 5 CDK5 Marker is a distinct antibody that targets the human protein Cdk5. The antibody recognizes the activity of Cdk5, which is an essential regulator of nerve growth and development. Cdk5 is abundantly expressed in the development process and is believed to play a part in the axon's outgrowth process in Drosophila embryos.

The kinase is also known as PSSALRE and is crucial in controlling cell cycle transitions in the eukaryotes. It phosphorylates tau and high-molecular-weight neurofilaments. It is essential for proper development and regulation of postmitotic neurogenesis in mammalian central nervous systems.

This antibody recognizes Cdk5 in the position S453 within PIPKIg90. The antigen is expressed in MDA-MB-231 cells. The immunoprecipitation process was carried out using agarose beads containing anti-FLAG. The antibody phosphorylates PIPKIg90 at Ser453.

Boster Bio Anti-Cyclin dependent-like Kinase 5 CDK5 marker is available as an anti-Cyclin antigen under the catalog number PA1548. This antibody has been tested in a variety of ways and can be stored at -20°C for up to a year. The Boster Bio Anticdk5 Marker reacts with Rat, Mouse and Human cells.

Cyclin-dependent Kinase 5 (CDK5) is involved in various neuronal roles. It is also believed to be a permanent activated kinase. It is an enzyme that's activity is contingent on its interaction with non-cyclin cofactors. CDK5 is active most in postmitotic neurons and is cleaved through the calpain. Moreover, abnormal expression of CDK5 has been suggested to be a factor in the progression of tumors and metastasis.

After Cdk5 elimination, PIPKIg90S453A expression was decreased in MDA-MB-231 cell lines. Cells that were PIPKIg90S453E deficient were not able to produce PIPKIg90S453E. This suggests that Cdk5 negatively regulates PIPKIg90 phosphorylation.

This antibody recognizes the human CDK5 protein (p35). The cyclin-dependent kinase family of proteins which includes CDK5 as well as PPARgamma-receptor kinase, regulates this protein. These proteins control nerve cell movement, neurite growth and spine morphogenesis.

Applications

Angiogenesis is a procedure that provides oxygen and nutrients to tumors that are growing. The CDK5 gene plays a crucial role in this process. Studies have suggested that CDK5 is an important therapeutic target in the treatment of neurodegenerative diseases, including Alzheimer's disease. It also phosphorylates CRMP2, which is a mediator protein of the collapsin reaction that has been linked with the development neurofibrillary knots.

CDK5 is a key protein that is essential for the formation of lymphatic vessels. Its overexpression and aberrant activation have been linked to neurodegenerative diseases like Parkinson's and Alzheimer's. It plays a role in regulating the expression of connexin 37 which is crucial for the development of lymphatic valves. In mice, knockout of CDK5 causes lymphedema as well as embryonic lethality.

In addition to being a critical component in cancer, CDK5 plays a essential regulator of a variety of biological processes, including apoptosis angiogenesis and migration, as well as immune escape. When cells are exposed to DNA damage, CDK5 increases the expression of genes such as the p35 and p39 genes. N-Myc and Menin are transcriptional factors directly involved in the production of p35. Both are involved in synaptic plasticity.

CDK5 regulates cell adhesion and cytoskeletal dynamic, two important functions during development and in the adult system. Aberrant activation of CDK5 by p25 causes neurodegenerative disorders and deregulation of the protein leads to disruption of cell positioning. Moreover the Cdk5 family of proteins is involved in cell division and has received considerable attention in the field of treatments for cancer. The CDK5 gene, which is involved in the development and maintenance of postmitotic neuron is a significant therapeutic target for neurodegenerative diseases.

CDK5 has been linked not just in tumorigenesis, but also in its function. It has been established that activation of DRP1 is associated with a lower prognosis in patients with glioblastoma. Further investigations are needed to determine the spatiotemporal dynamics of activation of CDK5. However, the CDKACT5 peptide biosensor library was created by researchers to identify Cyclin-dependent Kinases and actin band.