Ccdc80 Antibodies

Coiled-coil domain-containing protein 80 (Ccdc80)

Promotes cell adhesion and matrix assembly. .

.

Gene Information

Rat
Gene Name:	Ccdc80
Uniprot:	Q6QD51
Entrez:	64387

Family

Belongs to:
CCDC80 family

Alternative Names

CCDC80; coiled-coil domain containing 80; down-regulated by oncogenes protein 1; DRO1; SSG1; Steroid-sensitive protein 1; URB

Molecular Weight

Mass (kDA):
107.691 kDA

Genomic Context

Rat
Location:	11q21
Sequence:	11;

Tissue Specificity

Isoform 2 is expressed in uterus, liver, lung, spleen, kidney, heart, bladder, skeletal muscle and brain (at protein level). Isoform 2 is expressed very low in mammary gland and intestine (at protein level). Isoform 2 is expressed in lactating mammary glands and mammary tumors (at protein level). Ubiquitous (isoform 1). Isoform 2 is expressed in ovary, uterus, mammary glands, liver, lung, spleen, kidney, heart, bladder, intestine, skeletal muscle and brain.

Diseases

• Neoplasms
• Obesity
• Dermatitis
• Dermatitis, Atopic
• Muscular Atrophy
• Glioblastoma
• Atrophy
• Hypoxia
• Malignant Neoplasms
• Nervousness

• Hyperglycemia
• Mammary Neoplasms
• Impaired Glucose Tolerance
• Dermatologic Disorders

Pathways

• Secretion
• Glucose Homeostasis
• Reverse Transcription
• Cell Proliferation
• Cell Cycle
• Axon Guidance
• Insulin Secretion
• Somitogenesis

• Feeding Behavior
• Energy Homeostasis
• Muscle Atrophy

PTMs

• Glycoprotein
• Isopeptide bond

• Phosphoprotein
• Ubl conjugation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Ccdc80

Best Uses Of The CCDC80 Marker

To measure CCDC80 levels in plasma, you need to know how the blood circulates. This article will talk about the benefits of this marker, as well the ELISA analysis performed by Boster Bio's CCDC80Marker. We'll also be discussing IGF1, another marker of CCDC80. We'll discuss its use in the IVF research field and its relationship to Boster Bio's CCDC80 Marker.

Human plasma contains CCDC80 protein

Boster Bio's CCDC80 proteins have been shown to circulate within human plasma and have systemic effects. CCDC80's association with high levels C-reactive protein (a marker of atherosclerosis) and thick carotid intima media suggests that it may play an important role modulating glucose and energy homeostasis. The protein is also a candidate adipokine that may act on the metabolism of fat, a possible role in the prevention of cardiovascular disease.

The Human CCDC80 ELISA kits provides quantitative measurement for the CCDC80 protein content in serum and cell cultures supernatants. The human CCDC80 genome is located at 3q13.2. It is classified under the peroxiredoxin family. The kit contains reagents suitable for 96 testing. The kit includes 96 strips and wells. Biotinylated goat antigen-binding antibody was used in the creation of this kit.

The anti-inflammatory effects of CCDC80 have been studied in association to a number of conditions such as insulin secretion, circulating insulin, and blood neutrophil counts. The study suggests that the circulating CCDC80 protein may be indicative of an inflammatory state that is independent of BMI. In addition, serum CCDC80 levels have been associated with inflammatory and metabolic liver diseases, as well as an increase in hepatic steatosis.

Researchers found that CCDC80 protein concentrations were significantly higher in obese than in lean subjects in VAT depots. Although CCDC80 protein was detected in both lean and obese subjects, its concentration was 3.9 times higher in the VAT depots of obese patients than in their lean counterparts. CCDC80 protein expression was also restricted to 150kDa species in plasma.

Benefits of using CCDC80 Marker

The serum levels CCDC80 protein were found to be correlated with liver and carotid steatosis. The levels were compared using box plots that reflected the presence of moderate, severe and severe hepatic impairments and the external carotid media thickness. Furthermore, the levels were found to be independently related to the anthropometric characteristics of the morbidly obese group and the normal weight cohort.

In a group of obese patients, serum CCDC80 levels were measured using enzyme-linked immunosorbent (ELISA). Serum CCDC80 levels were not significantly higher in obese patients than those of lean patients. They were negatively correlated with insulin production during OGTT as well as glucose levels 30 min later. The CCDC80 level was not related to BMI or free IGF-1.

ELISA analysis of Boster Bio CCDC80 Marker

To maximize the sensitivity of your ELISA experiments, you must be familiar with the Western Blot Principle. This guide will show you how to avoid artifacts from appearing on your Western Blot. Artifacts on Western Blots are most commonly caused by uneven buffer coating, membrane drying, or aggregates of antibodies. In addition to the Western Blot Principle, sample preparation is also very important.

The Human URB/CCDC80 ElISA Kit includes 96-well plates that have removable strips for measuring CCDC80. The kit detects CCDC80 in serum and cell culture supernatants. CCDC80 is a gene mapped to 3q13.2 and is classified as a peroxiredoxin. To perform the ELISA analysis, you must use a Microplate Reader capable of reading absorbance at 450 nm.

IGF1

CCDC80 is a highly expressed gene that can be found in blood and urine of patients with cancer. There are several ways to measure the level of CCDC80 in human and mouse blood samples. The most widely used method for CCDC80 measurement is the enzyme–linked immunosorbent asay (ELISA). This method employs a monoclonal antibody that contains detection antibodies against the full length recombinant protein in order to identify the protein.

A study of obese individuals measured the serum CCDC80 level. This marker was not significantly different from those of lean individuals. It negatively correlated with IGF-1 and acute insulin response to glucose levels. It was positive associated with the blood count of neutrophils. The study concluded that serum CCDC80 levels are associated with the risk of diabetes and obesity.

CCDC80's function is not completely understood. Previous research suggests that it may be involved regulating glucose and energy homeostasis. However, CCDC80 was not detected in serum in previous studies in mice. Interestingly, CCDC80 immunoblotting in control human plasma revealed two major species, one of 75 kDa and the other of 150 kDa. This latter form is secreted into human blood.

CCDC80 is one of many markers that can be used. It has been associated with metabolic disorders. CCDC80 protein levels were higher in obese patients. CCDC80 also positively associated with glucose tolerability derangements, inflammation markers, and adipokines. These results suggest that CCDC80 could be a useful biomarker for the fight against obesity and chronic diseases.

CD14

The CCDC80 mRNA mark is a highly-specific marker that binds with the amino acids sequence 840-854 within the OVCA Gene. It is expressed in OVCA-induced adipogenesis cells and human ovarian cancer cells. We used this mRNA in the present study for identification of the target gene CCDC80 from the mouse OVCA line 3T3L1.

The expression of CCDC80 in OVCA tissues was compared to that of non-OVCA tissues using the CIBERSORT algorithm. The results revealed that high CCDC80 expression was associated with the fraction of infiltrating naive B-cells and a score on B-cell-related pathways. CCDC80 could be a tumor suppressor through its effects on B-cells. Further studies are needed in order to discover if CCDC80 may be associated with tumor-specific immune systems.

CCDC80 has been identified as a key marker in the development and progression of pulmonary disease. Its expression can be linked to pulmonary vessel tone and regulates endothelin-1-induced collagen expression. This protein could also affect the tone of the pulmonary arteries. Its role is unknown in endothelial function. Its role in PAH-induced lung disease is crucial for clinical diagnosis.

The CCDC80 marker is a crucial protein in preadipocyte differentiation and is responsible for a switch in Wnt/b-catenin signaling. Modifying Ccdc80 gene expression in different cells may allow for new strategies to combat obesity and prevent it. Ccdc80 promotes cell differentiation when expressed correctly in adipocytes. CCDC80 inhibits TOPFLASH reporter from human adipocytes by activating it in human adipocytes.