CAPN1 Antibodies

Calpain-1 catalytic subunit (CAPN1)

Calcium-regulated non-lysosomal thiol-protease that catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. .

Gene Information

Human
Gene Name:	CAPN1
Uniprot:	P07384
Entrez:	823

Family

Belongs to:
peptidase C2 family

Alternative Names

Calcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; Calpain mu-type; calpain, large polypeptide L1; calpain-1 catalytic subunit; Calpain-1 large subunit; CANP 1; CANP; CANPL1EC 3.4.22.52; Cell proliferation-inducing gene 30 protein; cell proliferation-inducing protein 30; EC 3.4.22; micromolar-calpain; muCANPCANP1; muCL

Molecular Weight

Mass (kDA):
81.89 kDA

Genomic Context

Human
Location:	11q13.1
Sequence:	11; NC_000011.10 (65181373..65212006)

Tissue Specificity

Ubiquitous.

Subcellular Localizations

Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. In granular keratinocytes and in lower corneocytes, colocalizes with FLG and FLG2 (PubMed:21531719).

Diseases

• Sore To Touch
• Neoplasms
• Cholangiocarcinoma
• Malignant Neoplasms
• Nerve Degeneration
• Tissue Adhesions
• Alzheimer's Disease
• Malaria
• Endocrine Gland Neoplasms
• Infarction
• Multiple Endocrine Neoplasia Type 1
• Multiple Endocrine Neoplasia

• Carcinoma
• Muscle Tenderness
• Brain Injuries
• Abnormal Degeneration
• Ischemia
• Diabetes Mellitus
• Inflammation

Interacting Proteins

• EZR
• PTPN1
• STAT3

Pathways

• Cell Death
• Proteolysis
• Aging
• Cell Migration
• Pathogenesis
• Cell Adhesion
• Hyperphosphorylation
• Localization
• Autophagy
• Secretion
• Cell Motility
• Cell Differentiation
• Cell Proliferation

• Muscle Atrophy
• Platelet Aggregation
• Cell Cycle
• Skeletal Muscle Atrophy
• Myoblast Proliferation
• Mammary Gland Involution
• Membrane Organization

PTMs

• Cleavage
• Phosphorylation
• Oxidation

• Acetylation
• Dephosphorylation
• Ribosylation

• Ubiquitination

Research Areas

• Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CAPN1

Best Uses Of The CAPN1 Marker

The most effective method to determine whether a cell contains Caspase 1 is to perform an ELISA test. The cell membrane carries the Caspase 1 marker, which is known as the CAPN1 marker. We will explore the functions Caspase 1 does and how it influences cell proliferation in this article. We will also discuss the importance of Caspase 2 3, 4 and.

Caspase 1

The expression of Caspase-1 has been shown to play a significant role in the progression of breast cancer. It has been discovered to impact the proliferation and apoptosis and invasion of breast cancer cells. Because it is a low expression marker, Caspase-1 may provide a novel target molecule for drugs. Additionally the molecule's low levels are not affected by competing interests.

The protein is present in a variety of tissues, including tissue and cells. Biological assays employ monoclonal and polyclonal antibodies to identify Caspase-1. This protein is involved in a variety functions such as defense against pathogens, and also promoting apoptosis. The enzyme is located in the liver as well as the spleen, small intestine, and peripheral blood leukocytes.

Caspase 2

Boster Bio offers ELISA kits based upon the CAPN1 biomarker. These kits can be used to determine biomarkers in the field of developmental biology, inflammation, cancer and neurosciences. They come with a variety of highly sensitive antibodies comprising a single molecule to thousands of Recombinant proteins. The biomarker kits can achieve levels of sensitivity that are picogram-level. Boster Bio immunological Reagents are available from tebu-bio.

In addition to biomarker research in addition to biomarker research CAPN1 gene is also involved in the process of meat tenderization. It has been linked to the development of postmortem aging and melanoma. Utilizing this marker to assess the expression of CAPN1 in meat is a first step in better understanding how the protein affects the quality of meat. It is important to know more about the function of this gene in the food processing.

Caspase 3

The CAPN1 marker is a single nucleotide polymorphism located in the genome of cattle. It is involved with the tenderization of meat and the associated postmortem aging. Boster Bio has information about this marker and other boster marks used for various research objectives. It is also a good choice for custom services. It is recommended to speak with the manufacturer regarding the most effective methods and controls.

Antibodies for the CAPN1 Marker are produced by Boster Bio. They are extremely specific for this biomarker, and are optimized for IHC and ELISA. They have an excellent affinity and are tested against 250 different tissues. Additionally, they are examined quantitatively against known recombinant proteins , as well as untransfected cells.

Caspase 4

CAPN1 plays a major role in meat tenderness when it is studied in cattle genomes. A variety of studies have revealed that it is associated with better meat tenderness in beef and pork. The role of the CAPN1 genes is more complex. The CAPN1 gene is responsible for regulating the expression of specific proteins and is highly preserved in cattle species. This means that the CAPN1 gene is involved in a variety of processes, such as tenderization of meat and postmortem ageing.

Boster Bio has developed the CAPN1 antibody ELISA Kit to identify biomarkers in multiple fields. These ELISA kits are highly sensitive and can detect levels of CAPN1 right down to the picogram level. Boster Bio has developed several immunological reagents to detect this molecule available through its distributor, tebu-bio. Boster Bio's product CAPN1 is available in various concentrations and spectrums.

Caspase 5

Caenorhabditis elegans, a member of the Caenorhabditis elegatans superfamily, is a well-known cancer biomarker. Boster Bio provides ELISA kits for detection of the protein. The products have been tested against 250 human tissues to ensure sensitivity to immunochemicals. They have been tested against recombinant proteins in known quantities to ensure their accuracy and quality. Boster Bio also offers immunological Reagents through its online store, Tebu-bio.

The tumorigenesis of cancer is mediated by the CAPN1 gene. Thus knockdown of CAPN1 could trigger the apoptosis process to occur. The flow cytometry technique was used to determine whether CAPN1 knockdown was occurring. The proportion of Annexin V and PI-positive cells in calpain1 cells transfected with siRNA was 12+-0.16 percent and 14+-0.51% in siRNA-negative cells. The two populations did exhibit significant differences (P=0.8593).

Caspase 6

Boster Bio's ELISA and research antibodies to detect CAPN1 were developed to aid in the identification of biomarkers in cancer, neurosciences and development. The kits are sensitive to the level of a picogram. Boster Bio offers immunological reagents as well as ELISA kits. Boster Bio products can be purchased from their website tebu.bio.

Caspase 7

Boster Bio is an antibody manufacturer that produces high-quality ELISA kits and research antibodies. These kits detect biomarkers that are related to cancer, development and neurosciences, as well as inflammation. Their immunological reagents have been validated against a panel of over 250 tissues. The antibodies in Boster Bio kits have a high affinity for the target protein and have been confirmed quantitatively against the known quantities of recombinant proteins. The company's line ELISA kits are available at tebu.bio.

Below are the most frequent applications of the CAPN1 gene in food and animal nutrition production.

Caspase 8

The CAPN1 marker is used for a variety of purposes in the field of research and medicine. It is used to identify proteins that can aid in the diagnosis of neurodevelopmental biology, inflammation and various other disorders. It can be used to determine postmortem ageing, cancer and other purposes, based on the specific application. The boster bio-based ELISA kit is designed to identify specific molecules within the body.

Caspase 9

The CAPN1 marker has recently been identified as a potential gene for cancer and is a useful biomarker that can be used to assess the aging process of meat. It has been linked with meat tenderness after postmortem aging. Boster Bio: Best Uses for The CAPN1 Marker

Caspase 10

Caspase-10 isoforms have been identified as crucial factors in FasL and TRAIL-mediated cell death. Both are extremely resistant to apoptosis, but the distinct modulation of expression of caspase-10 could help them be more sensitive to apoptosis-inducing medications. Which one of them should be used in clinical trials? Let's take a closer look at these two isoforms.

The Caspase-10 kit analyzes the amount of Caspase-10 within cells in a straightforward and easy way. The kit works through hydrolysis, releasing pNA out of the labeled substrate. This moiety has a maximum absorbance of 405 nanometers and therefore the pNA concentration can be calculated. The kit includes the pNA dye standard microplate reader, and the spectrophotometer for measuring Caspase-10 activity.