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- Table of Contents
Facts about Complement C1r subcomponent.
Human | |
---|---|
Gene Name: | C1R |
Uniprot: | P00736 |
Entrez: | 715 |
Belongs to: |
---|
peptidase S1 family |
C1r; complement C1r subcomponent; Complement component 1 subcomponent r; complement component 1, r subcomponent; Complement Component C1r; EC 3.4.21; EC 3.4.21.41
Mass (kDA):
80.119 kDA
Human | |
---|---|
Location: | 12p13.31 |
Sequence: | 12; NC_000012.12 (7080219..7092445, complement) |
Secreted.
If you're new to immunology, it may seem overwhelming to choose a high-affinity primary antibody. Boster has many options. Boster offers more than just its highly-regarded antibody selection. They also offer a variety other products such as C1R marker, Fab fragments and high-affinity secondary antibodies. Let's look at these options and see what they offer.
Flow cytometry requires high-affinity primary antibodies. This process allows scientists the ability to examine the composition of a sample including cells, molecules and particles. Boster Bio offers monoclonal, and polyclonal antibodies to help scientists perform flow cytometry. These antibodies are highly cited, and have been around for more than 25 years. It is important that you understand the purpose and function of these antibodies in order to maximize their potential.
Boster Bio has developed a proprietary PCD platform to generate high-affinity clones. These clones exhibit the highest specificity, making them ideal for Diagnostics & Therapeutics targets. Boster Bio PCD technology employs flow cell cytometry combined with proprietary chemistry to prevent B cells (and other cells) from secreting antibodies. They are also retained on their cell membranes. After the antibodies are isolated, splenocytes and plasma cells are incubated with flurochrome-conjugated antigens and the brightest clones are separated for downstream screening.
To make high-affinity primary antibodies, scientists must first create the antigens. In some cases, polyclonal antibodies can be used as a stepping stone to monoclonal antibodies. Because polyclonal antibodies can be used in scientific research, they are acceptable to be tested and a good start in the right direction of immunological diagnostics. Aside from monoclonal antibodies, polyclonal primary antibodies also have a wide range of potential uses, including the detection and purification of biomarkers and secreted proteins.
ELIS assays work in the same manner as other immunoassay techniques: specific antibodies bind specifically to a target protein. A detection system then determines whether the antigen has been bound. High-affinity antibodies are essential for enzyme-linked immunosorbent assays, and Boster Bio is an expert at this process. Scientists around the globe trust these antibodies. So, if you're working with ELISAs, consider using Boster Bio high-affinity primary antibodies to enhance your success rate and sensitivity.
To confirm the specificity of an antibody, the manufacturer must validate the product. The customer then selects the three most promising clones from supernatant. Each clone includes ELISA data as well as a map of vector and purified antibody. Alternatively, the customer may choose to select the best three clones for testing purposes. They also receive 100 ng of heavy-chain plasmid DNA.
Researchers can use a variety assays with the unique combination, high-affinity secondary antibodies. Primary antibodies are the core of many experiments and assays like Western Blotting. There are also immunoassays such as immunohistochemistry or histopathology. Flow cytometry measures the number a particular cell type. Researchers can learn more about specific cells by using these antibodies.
Researchers are not the only ones who have ever wondered how to use C1R Marker in their experiments. There are many flow methods and questions to consider when using C1R Marker in experiments. Boster Bio optimization tips can help you design and optimize experiments. Learn more about this biomarker. Follow the appropriate protocol for each experiment.
PMID: 3021205 by Leytus S.P., et al. Nucleotide sequence of the cDNA coding for human complement C1r.
PMID: 3030286 by Journet A., et al. Cloning and sequencing of full-length cDNA encoding the precursor of human complement component C1r.