BHMT Antibodies

Betaine--homocysteine S-methyltransferase 1 (BHMT)

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively.

This reaction is also required for the irreversible oxidation of choline. .

Gene Information

Human
Gene Name:	BHMT
Uniprot:	Q93088
Entrez:	635

Family

Belongs to:
No superfamily

Alternative Names

betaine homocysteine methyltransferase; betaine-homocysteine methyltransferase; betaine--homocysteine S-methyltransferase 1; betaine--homocysteine S-methyltransferase; BHMT1; EC 2.1.1; EC 2.1.1.5

Molecular Weight

Mass (kDA):
44.998 kDA

Genomic Context

Human
Location:	5q14.1
Sequence:	5; NC_000005.10 (79111809..79132288)

Subcellular Localizations

Cytoplasm.

Diseases

• 5,10-methylenetetrahydrofolate Reductase Deficiency
• Hyperhomocysteinemia
• Cardiovascular Diseases
• Malignant Neoplasms
• Liver Carcinoma
• Vascular Diseases
• Carcinoma
• Neoplasms
• Folic Acid Deficiency
• Fatty Liver
• Diabetes Mellitus
• Congenital Abnormality
• Thrombosis

• Cleft Palate
• Liver Diseases
• Steatosis
• Liver Neoplasms
• Carcinogenesis
• Cleft Lip
• Disease Of Adrenal Medulla

Pathways

• Methylation
• Dna Methylation
• Transsulfuration
• Transport
• Secretion
• Proteolysis
• Pathogenesis
• Excretion
• Cell Death
• Autophagy
• Aging
• Macroautophagy
• Cell Proliferation

• Response To Alcohol
• Localization
• Dna Hypomethylation
• Amino Acid Homeostasis
• Nodulation
• Reverse Transcription
• Mrna Transcription

PTMs

• Methylation
• Oxidation

• Reduction
• Cleavage

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/BHMT

Best Uses Of The BHMT Marker

Boster offers a variety of primary antibodies that are highly specific for BHMT. Boster antibodies are known for their reliability in research and are widely cited and trusted by the scientific community. Their antibodies are validated on Western Blotting, Immunohistochemistry, and ELISA. They are used for studies in cell culture as well as tissue samples and cancer cells. Boster antibodies are available in ELISA kits or as primary antibodies of high affinity.

Kits for the ELISA

ELISA Kits for BHM Marker measure BHLHB3 levels in plasma, serum homogenates of tissues, and cell lymphosates. These kits can also be used to measure BHLHB3 levels within cell culture supernats or other biological fluids. All of these tests require high levels of BHLHB3 in order to measure the amount of the marker present in a sample.

The MBS2104954 microwell strip-plate ELISA Kit is used to detect the BHMT marker within biological samples. This kit is able to detect the native BHMT2 antigen. It is suitable for use with body fluids that are not diluted or tissue homogenates. It can be used to conduct routine research or specialized research and has a sensitivity that is greater than 99.9%.

WB/IHC Compatible Antibodies

If you are looking for WB/IHC-compliant antibodies, Boster Bio is the place to turn. Boster Bio, a company that specializes in immunological reagents is a leader in the supply of high-quality antibodies. Boster Bio offers many services, including custom mouse monoclonal antibodies and multiplex cytokine assays. Boster Bio offers a wide assortment of WB/IHC-compatible antibody and ELISA kits.

Based on epitope recognition, monoclonal antibodies have low affinity, a high background and high cross-reactivity. However, this versatility often results in significant technical expertise. Monoclonal antibody production has been done in labs using DNA sequences. They are more resistant to harsh washing conditions and less sensitive to antigen differences. They are also more likely to show variations from batch to bath than polyclonal antibodies.

Immunohistochemistry utilizes antibodies to detect antigens in tissue. A fluorescent dye or colored enzyme substrate is used to show the interaction between an antibody and an antigen. Although immunohistochemistry cannot provide quantitative information, the patterns of protein expression can be helpful diagnostic tools for pathologists. However, proper staining and high-quality specific reagents are essential for the best results.

Monoclonal antibodies are the most popular type. They are helpful in testing for antibodies in tissues that are infected with a specific antigen. They are difficult to wash out because they are not able to be thoroughly washed. Boster Bio WB/IHC Compliant Antibodies could be a boon. You'll be amazed at how much easier it is to recognize cancer using these antibodies.

Primary antibodies with high affinity

Boster Bio high-affinity prima antibodies are a good choice for those who need to test specific antibodies for biological activity. They offer rabbit polyclonal antibodies and are approved for use in IHC and WB. Additionally, they offer an additional antibody for free with the purchase of an initial antibody. They have been referenced in numerous scientific publications and have a long shelf-life.

To test this hypothesis, researchers used a representative set of 50 Abs from the two previous vaccinations. The data were arranged around clearly defined mean values. The temperature variation did not affect the geometric mean of kon, but it did alter the geometric mean of koff by 2.4-fold. This results in a decrease of the average interaction t1/2. However, the mean affinity of koff was unaffected by temperature.

High-affinity primary antibodies from pigs have been identified with significant affinity for the SARS-CoV-2 receptor binding domain. They were analyzed using flow cytometry. The antigen-specific proteins were immobilized to magnetic beads, and the antibodies bound to them. Three independent experiments were conducted to determine the half-maximal effective levels of high-affinity antibodies (12H2 13I1), and the positive control antibody (CB6).

The process of developing primary antibodies of high affinity usually takes years. Scientists immunize host animals using antigens before removing antibodies from their sera or eggs. Typically, the antibodies are produced by a process known as polyclonal. This method is used to create antibodies that have high affinity for targets like COVID-19. They are multi-functional and can be utilized in a variety of applications.

Using a fluorescent-conjugated anti-human IgG1 as a control, Boster Bio's high-affinity primary antibodies have excellent affinity. This means you can use them in flow cytometry. This technique lets you evaluate the effectiveness of your research by detecting specific cells. Boster Bio's high affinity primary antibodies are extremely useful for research and can be used in any laboratory setting.

ExpiCHO cells produced the mAb5D5 using a mouse IgG1 fusion proteins and AVITag as its N terminal. After purification 5D5 Fab was biotinylated according to the procedure described. In addition, scFab constructs were designed with two light chains joined by a linker of 72 residues. The monoclonal antibody resulting was isolated using KappaSelect affinity chromatography.

The company also provides customized services for antibodies. Using a eukaryotic expression system it produces polyclonal antibodies and monoclonal antibody for use in animal models. It also provides high-throughput antibody manufacturing. They also offer antibody development services, like humanization, custom Fc and Fv optimisation. Biogenes is a good option if you need a monoclonal anti-body cell line. They can produce antibodies in rabbits, chicken or human. They can also be conjugatedto allow you to use them in your laboratory and then test the antibody for your personal use.