AFAP1 Antibodies

AND AFAP1 ELISA kits, AFAP1 Proteins

Many biological assays use antibodies to detect AFAP1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with AFAP1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop AFAP1 antibodies...

We validate the specificity of these antibodies to AFAP1 by testing them on tissues known to express AFAP1 positively and negatively. Browse below to find the AFAP1 antibody that suites your experiment. We have 6 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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AFAP1 Infographic by Boster Bio

All AFAP1 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-AFAP1 Antibody

Human, Mouse

ELISA, WB

$449
Cat # A05258
0.1 mg

Anti-AFAP1 Antibody

Human, Mouse, Rat

IF, ICC, WB

$405
Cat # A05258-1
100ul

Anti-AFAP/AFAP1 Antibody Picoband®

Human, Mouse, Rat

ELISA, Flow Cytometry, IHC, WB

$370
Cat # A05258-3
100 μg/vial

Human AFAP Recombinant Protein

SDS-PAGE, Denatured

$250
Cat # PROTQ8N556
Starting from 10ug

Anti-AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI2G9]

Human, Mouse, Rat

IHC, WB

$523
Cat # M05258
100 µl

AFAP (AFAP1) (NM_021638) Human Over-expression Lysate

Human

$628
Cat # LS060312
100 µg

AFAP1 Overview

Facts about Actin filament-associated protein 1.

AFAP1 3D structure. Source: alphafold

Actin filament-associated protein 1 (AFAP1)

Can cross-link actin filaments into both network and package structures (By similarity). May regulate changes in actin filament integrity and cause lamellipodia formation.

May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.

Gene Information

Human
Gene Name:	AFAP1
Uniprot:	Q8N556
Entrez:	60312

Family

Belongs to:

No superfamily

Alternative Names

actin filament associated protein 1; AFAP; AFAP1; AFAP-110; FLJ56849

Molecular Weight

Mass (kDA):
80.725 kDA

Genomic Context


Human

Location:	4p16.1
Sequence:	4; NC_000004.12 (7758713..7939926, complement)

Tissue Specificity

Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF- 7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma.

Subcellular Localizations

Cytoplasm, cytoskeleton, stress fiber.

Diseases

Myopathy
Neuroblastoma
Neoplasm Metastasis
Malignant Neoplasms
Fetal Diseases
Neuromuscular Diseases

Pathways

Cell Proliferation
Cellular Localization
Cell Motility
Intracellular Signal Transduction
Localization

PTMs

Phosphorylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/AFAP1

References

PMID: 15485829 by Han B., et al. Conversion of mechanical force into biochemical signaling.

PMID: 17520695 by Dorfleutner A., et al. AFAP-110 is required for actin stress fiber formation and cell adhesion in MDA-MB-231 breast cancer cells.