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- Table of Contents
Facts about Short-chain specific acyl-CoA dehydrogenase, mitochondrial.
less than 6 carbon atoms. .
Human | |
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Gene Name: | ACADS |
Uniprot: | P16219 |
Entrez: | 35 |
Belongs to: |
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acyl-CoA dehydrogenase family |
ACAD3; acyl-CoA dehydrogenase, C-2 to C-3 short chain; acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain; Butyryl-CoA dehydrogenase; EC 1.3.99; EC 1.3.99.2; mitochondrial short-chain specific acyl-CoA dehydrogenase; SCADshort-chain acyl-CoA dehydrogenase; short-chain specific acyl-CoA dehydrogenase, mitochondrial; unsaturated acyl-CoA reductase
Mass (kDA):
44.297 kDA
Human | |
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Location: | 12q24.31 |
Sequence: | 12; NC_000012.12 (120725826..120740008) |
Mitochondrion matrix.
The ACADS Marker ELISA marker is a highly sensitive and unique ELISA probe for protein levels within SO-RB50 cells. This article will explain the science behind the ACADS Marker, and highlight some of Boster Bio's best-selling products. Here are the best uses for the ACADS Marker. Continue reading to find out more. You will also find out how Boster Bio can be funded through research grants, financial aid, and other financial aid.
This assay was performed in Transwell chambers using a standard transfection method. The apical sides of the chambers were coated in Matrigel. Invasion assays were performed with the same procedures as migration assays. To act as a chemoattractant DMEM was added to a basal compartment with 20% FCS. Cells were allowed to migrate for 24 h before being scraped off using a wet cotton swab. To determine migration towards DNP, flow cytometry was used. The cells were stained by 0.2% crystal violet.
The use of ACADS-based Western blotting kits requires proper sample preparation. Samples are prepared using specialized cell lysis buffers, and protease inhibitors. The method of extraction used depends on the sample type. The most commonly used methods of tissue preparation are homogenization or sonication. However, other techniques include detergent and osmotic shocked. For proteins present in mitochondria, radioimmunoprecipitation assay buffers are better suited. Denatured samples might not be detectable with antibodies so specialized buffers are required.
The gel should contain equal amounts of protein. Next, the gel is electrophoretically transferred with equal amounts of samples to a membrane. This step is critical as mistakes can cause poor recovery and degradation of proteins. Towbin buffer, the standard transfer buffer, can be adjusted to match the target protein. If you carefully follow the protocol, you should be capable of detecting the protein.
The Bradford assay is a common method for quantitative Western Blotting. It measures the amount of protein in the gel. This method exploits the interaction between proteins and dyes. These are detected using spectrophotometers. After calculating the target protein's content, the result is compared with that of the housekeeping proteins. The resulting analysis is usually highly quantitative, especially when using fluorescent Westerns.
SDS concentrations, buffer compositions, and blotting techniques can all influence the sensitivity to detection. Low detection efficiency can also lead to lower sensitivity. Semi-dry transfers can also fail to detect higher molecularweight proteins. A discontinuous buffer system, however, can improve the transfer rate of higher molecular mass proteins. This is not recommended in quantitative Western.
Nitrocellulose-based membranes are also available. They are not compatible with SDS-containing transfer buffers and can result in protein binding to the membrane. These membranes are also not suitable for fluorescent Westernblots. The problem with Nitrocellulose-based membranes, however, is their fragility and tendency to break easily when they are handled often. There are two main types: 0.45 mm or 0.2 mm.
The ACADS marker was used to measure levels of extracellular protein band 6, also known as ABAS, within SO-RB50 cells. This band is responsible to the secretion ABAS, a cytokine important for fibroblast differentiation. This ABAS marker is highly specific for this protein. Its molecular mass, 40.4 kDa.
B. bronchiseptica RB50 secretes a wide variety of proteins. Bsp22, which is found in both iron rich and iron deplete conditions, was the most abundant supernatant protein. The protein bands were extracted from SO-RB50 cultures and analyzed using reverse-phase liquid chromatography-tandominant mass spectrometry. LC-MS/MS confirmed the presence of BB1617 protein.
So-Rb50 were grown on poly-d–lysine-coated cover slips and incubated with 1% oxygen or 2% oxygen. After incubation SORB50 cells had been fixed with PEDF (parapheresis inhibitor). The SO-Rb50 cell were then incubated with a 100 nmol/L of PEDF solution for 10 h. We used an anti-HIF-1a antibody purchased from Santa Cruz Biotechnology and Image Plus Pro 6.0 software to perform the experiments.
Knockdown LINC00152 causes RB cells to become less proliferative, migrate, invade, and apoptosis-resistant. The production of Bcl-2 and Ki-67 as well as MMP-2 is significantly reduced by knocking down LINC00152. These proteins are known to reduce RB tumor growth both in vitro, and in vivo.
A previous study had shown that the RNA of LINC00152 in RB50 cell culture is overexpressed in inflammatory fibroblasts. However, results showed inconsistent results between laboratory-adapted and clinical strains. It is important for you to know that RNA expression does not directly correlate to cytokine levels. LINC00152 overexpression can also occur in a bacterial cell line.
LINC00152 a 828 base-pair lncRNA is associated with multiple kinds of cancers. It is not clear what role this lncRNA plays in RB cells. However, it has been shown to regulate a variety of RNAs. We examined LINC00152's expression in SO-RB50 cells and its molecular mechanism.
Students can get funding for their education through the Bosterbio Scholarship Program. It is open to students in four year academic programs and medical schools. Boster Bio products include buffers, lysates, and extraction kits. The scholarship is open to students interested in medicine, biology, or veterinary medicine. If students are graduating from highschool, they can also apply for the scholarship.
The company produces its own antibodies as well as ELISA kits. Boster Bio's proven track record for high-quality products is backed by more than 20 years experience and more 14000 product citations. Boster Bio also conducts rigorous testing to make sure its products work as they are promised. Its antibodies have been tested on human tissues as well as recombinant proteins. Boster Quality Guarantee assures that every product performs as promised.
PMID: 2565344 by Naito E., et al. Molecular cloning and nucleotide sequence of complementary DNAs encoding human short chain acyl-coenzyme A dehydrogenase and the study of the molecular basis of human short chain acyl-coenzyme A dehydrogenase deficiency.
PMID: 9383286 by Corydon M.J., et al. Structural organization of the human short-chain acyl-CoA dehydrogenase gene.