Research Spotlight - October 2022

Autumn is here and we’re happy to present our October issue of Research Spotlight!

This month, we highlight potential therapeutic technologies for treating various disorders. One study developed an exosome-based nanoplatform that enables CRISPR-Cas9 genome-editing therapy for liver diseases.

Other studies discuss non-drug approaches, such as proper sleeping patterns for preserving hematopoietic stem cell function and diversity, metabolic subtyping for guiding ketogenic therapy in colon cancer, and manual therapy for easing neuropathic pain.

In addition, several research papers examine the regulatory mechanisms of Tweak Receptor Fn14, miR21, IL-33, and NDRG1-OT1, the related signaling pathways, and the effects on different disorders or cancers.

Scroll down to learn more about these publications!

Sleep Exerts Lasting Effects on Hematopoietic Stem Cell Function and Diversity

Authors: McAlpine, C.S., Kiss, M.G., Zuraikat, F.M., Cheek, D., Schiroli, G…
Journal: Journal of Experimental Medicine

A sleepless night may feel awful in its aftermath, but sleep’s revitalizing powers are substantial, perpetuating the idea that convalescent sleep is a consequence-free physiological reset. Although recent studies have shown that catch-up sleep insufficiently neutralizes the negative effects of sleep debt, the mechanisms that control...

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Cited Boster Product(s): Mouse IL-6 ELISA Kit PicoKine® (EK0411); Mouse TNF Alpha ELISA Kit PicoKine® (EK0527); Mouse IL-1B ELISA Kit PicoKine® (EK0394); Mouse M-CSF ELISA Kit PicoKine® (EK0445); Mouse G-CSF / CSF3 ELISA Kit PicoKine® (EK0361)

Exosome-Mediated Delivery of Cas9 Ribonucleoprotein Complexes for Tissue-Specific Gene Therapy of Liver Diseases

Authors: Wan, T., Zhong, J., Pan, Q., Zhou, T., Ping, Y., Liu, X.
Journal: Science Advances

CRISPR-Cas9 gene editing has emerged as a powerful therapeutic technology, but the lack of safe and efficient in vivo delivery systems, especially for tissue-specific vectors, limits its broad clinical applications. Delivery of Cas9 ribonucleoprotein (RNP) owns competitive advantages over other options; however, the large size of RNPs...

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Cited Boster Product(s): Anti-TIP60 KAT5 Antibody (A01393)

Metabolism-Based Molecular Subtyping Endows Effective Ketogenic Therapy in p53-Mutant Colon Cancer

Authors: Tang, M., Xu, H., Huang, H., Kuang, H., Wang, C., Li, Q., Zhang, X…
Journal: Advanced Science

Although targeting cancer metabolism is a promising therapeutic strategy, clinical success depends on accurate molecular and metabolic subtyping. Here, this study reports two metabolism-based molecular subtypes associated with the ketogenic treatment of colon cancer: glycolytic (glycolysis+/ketolysis−) and ketolytic...

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Cited Boster Product(s): Plus RIPA Lysis Buffer (AR0102-100); SDS-PAGE Protein Loading Buffer 5X (Reducing) (AR1112)

Proteolytically Generated Soluble Tweak Receptor Fn14 is a Blood Biomarker for γ-Secretase Activity

Authors: Güner, G., Aßfalg, M., Zhao, K., Dreyer, T., Lahiri, S., Lo, Y…
Journal: EMBO Molecular Medicine

Fn14 is a cell surface receptor with key functions in tissue homeostasis and injury but is also linked to chronic diseases. Despite its physiological and medical importance, the regulation of Fn14 signaling and turnover is only partly understood. Here, we demonstrate that Fn14 is cleaved within its transmembrane domain by the protease...

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Cited Boster Product(s): Human TNFRSF12A/TWEAKR ELISA Kit PicoKine® (EK1753)

PVA/Pectin Composite Hydrogels Inducing Osteogenesis for Bone Regeneration

Authors: Hu, Z., Chen, J., Xu, S., Cheng, X., Zhao, J., Low, Z.W.K., Chee, P.L…
Journal: Materials Today Bio

Hydrogels composed from biomolecules have gained great interests as biomaterials for tissue engineering. However, their poor mechanical properties limit their application potential. Here, we synthesized a series of tough composite hydrogels from poly (vinyl alcohol) (PVA) and pectin for bone tissue engineering. With a balance of scaffold...

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Cited Boster Product(s): RIPA Lysis Buffer (AR0105-100)

miR21 Modulates the Hippo Signaling Pathway via Interference with PP2A Bβ to Inhibit Trophoblast Invasion and Cause Preeclampsia

Authors: Hu, M., Zheng, Y., Liao, J., Wen, L., Cheng, J., Huang, J., Huang, B…
Journal: Molecular Therapy: Nucleic Acids

Preeclampsia (PE) is a pregnancy-specific disorder attributed to deficient extravillous trophoblast (EVT) invasion into the uterus, but the mechanism of EVT invasion remains unclear. In this study, we found significantly elevated expression of miR21, which negatively regulates trophoblast invasion and migration, in preeclamptic...

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Cited Boster Product(s): DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) For Nucleic Acid Staining (AR1176)

Interleukin-33 Mediates Both Immune-Related and Non-Immune-Related Inhibitory Effects against Hepatitis B Virus

Authors: Gao, Z., Shen, Z., Wu, J., Song, Y., Liu, N., Deng, Q., Xie, Y., Liu, J.
Journal: Antiviral Research

Chronic infection by hepatitis B virus (HBV) is associated with high risks of liver fibrosis, cirrhosis and hepatocellular carcinoma. HBV covalently closed circular DNA (cccDNA) in the nucleus of infected hepatocyte serves as transcription template. Neither natural resolution of acute infection nor current treatment options for chronic...

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Cited Boster Product(s): Human IL-33 ELISA Kit PicoKine® (EK0929); Mouse IL-33 ELISA Kit PicoKine® (EK0930)

Regulatory Mechanisms and Function of Hypoxia-Induced Long Noncoding RNA NDRG1-OT1 in Breast Cancer Cells

Authors: Chao, H.H., Luo, J.L., Hsu, M.H., Chen, L.H., Lu, T.P., Tsai, M.H…
Journal: Cell Death   Disease

Hypoxia is a classic feature of the tumor microenvironment that has profound effects on cancer progression and is tightly associated with poor prognosis. Long noncoding RNAs (lncRNAs), a component of the noncoding genome, have been increasingly investigated due to their diverse roles in tumorigenesis. Previously, a hypoxia-induced...

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Cited Boster Product(s): Anti-Argonaute 2 AGO2 Rabbit Monoclonal Antibody (M00189)

Transcriptome Profiling of microRNAs Reveals Potential Mechanisms of Manual Therapy Alleviating Neuropathic Pain Through microRNA-547-3p-Mediated Map4k4/NF-κb Signaling Pathway

Authors: Yao, C., Ren, J., Huang, R., Tang, C., Cheng, Y., Lv, Z., Kong, L…
Journal: Journal of Neuroinflammation

Background: Local neuroinflammation secondary to spinal nerve compression in lumbar disk herniation (LDH) is a key driver contributing to neuropathic pain. Manual therapy (MT), a widely used nonsurgical therapy, can relieve LDH-mediated pain by reducing inflammation. MT has attracted extensive attention; however, its mechanism remains...

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Cited Boster Product(s): Rat TNF Alpha/Tumor Necrosis Factor ELISA Kit PicoKine® (EK0526); Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (EK0393); Rat IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0412)

Production of High Affinity Monoclonal Antibody and Development of Indirect Competitive Chemiluminescence Enzyme Immunoassay for Gentamicin Residue in Animal Tissues

Authors: Dai, P., Zhang, Y., Hong, Y., Xiong, J., Du, H., Duan, L., Wang, D…
Journal: Food Chemistry

To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin...

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Cited Boster Product(s): HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (BA1050)