Research Spotlight - November 2022

For our November issue of Research Spotlight, we’re happy to present 10 recent publications from scientists whom Boster has served.

Two research studies highlight new mechanisms by which BAX and serine metabolism orchestrate macrophage maturation as therapeutic strategies for macrophage-mediated diseases. For signaling pathways, scientists provided further insight into the roles of PPARs, AKT1, HOXA5, CDK12, and TOP2A in regulating cell differentiation, proliferation, and apoptosis as well as tumorigenesis.

In this issue, researchers also discussed the strong link between long-term exposure to atmospheric air pollutants and microplastics on neurotoxicity and cognitive dysfunctions.

Scroll down to find out more about each new publication!

Brain Milieu Induces Early Microglial Maturation Through the BAX-Notch Axis

Authors: Zhao, F., He, J., Tang, J., Cui, N., Shi, Y., Li, Z., Liu, S., Wang, Y…
Journal: Nature Communications

Microglia are derived from primitive myeloid cells and gain their early identity in the embryonic brains. However, the mechanism by which the brain milieu confers microglial maturation signature remains elusive. Here, we demonstrate that the baxcq55 zebrafish and Baxtm1Sjk mouse embryos exhibit similarly defective early microglial...

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Cited Boster Product(s): Anti-RBPJK/RBPJ Picoband™ Antibody (A00767-1)

Serine Metabolism Orchestrates Macrophage Polarization by Regulating the IGF1–p38 Axis

Authors: Shan, X., Hu, P., Ni, L., Shen, L., Zhang, Y., Ji, Z., Cui, Y., Guo, M…
Journal: Cellular & Molecular Immunology

Serine metabolism is reportedly involved in immune cell functions, but whether and how serine metabolism regulates macrophage polarization remain largely unknown. Here, we show that suppressing serine metabolism, either by inhibiting the activity of the key enzyme phosphoglycerate dehydrogenase in the serine biosynthesis pathway...

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Cited Boster Product(s): Mouse IGF-1 ELISA Kit PicoKine® (EK0378)

A Germline-Specific Regulator of Mitochondrial Fusion is Required for Maintenance and Differentiation of Germline Stem and Progenitor Cells

Authors: Zhang, R., Yu, Y.X., Ye, D., Gu, Z., Chen, Z.X., Sun, Y.
Journal: Advanced Science

Maintenance and differentiation of germline stem and progenitor cells (GSPCs) is important for sexual reproduction. Here, the authors identify zebrafish pld6 as a novel germline-specific gene by cross-analyzing different RNA sequencing results, and find that pld6 knockout mutants develop exclusively into infertile males...

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Cited Boster Product(s): Anti-Caspase-3/CASP3 Antibody Picoband™ (PB9188)

Effects of Legacy and Emerging Per- and Polyfluoroalkyl Substances on PPARα/β/γ Regulation and Osteogenic/Adipogenic Differentiation

Authors: Qin, H., Niu, Y., Luan, H., Li, M., Zheng, L., Pan, Y., Liu, W.
Journal: Environment International

As the primary molecular target, there is still a gap between the peroxisome proliferator-activated receptors (PPARs) regulation and the adverse health effects caused by per- and polyfluoroalkyl substances (PFASs). The effects of PFASs on cellular differentiation regulated by PPARs is likely significant given the association of PFASs...

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Cited Boster Product(s): DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) For Nucleic Acid Staining (AR1176)

Decrotonylation of AKT1 Promotes AKT1 Phosphorylation and Activation During Myogenic Differentiation

Authors: Qian, Z., Ye, J., Li, J., Che, Y., Yu, W., Xu, P., Lin, J., Ye, F., Xu, X…
Journal: Journal of Advanced Research

Introduction: Myogenic differentiation plays an important role in pathophysiological processes including muscle injury and regeneration, as well as muscle atrophy. A novel type of posttranslational modification, crotonylation, has been reported to play a role in stem cell differentiation and disease. However, the role of crotonylation...

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Cited Boster Product(s): HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (BA1050); HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054)

Evaluation of Neurotoxicity in BALB/c Mice following Chronic Exposure to Polystyrene Microplastics

Authors: Jin, H., Yang, C., Jiang, C., Li, L., Pan, M., Li, D., Han, X., Ding, J...
Journal: Environmental Health Perspectives

Background: The toxicity of microplastics (MPs) has attracted wide attention from researchers. Previous studies have indicated that MPs produce toxic effects on a variety of organs in aquatic organisms and mammals. However, the exact neurotoxicity of MPs in mammals is still unclear. Objectives: We aimed to confirm the neurotoxicity of...

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Cited Boster Product(s): Goat Anti-Rabbit IgG (H+L) Secondary Antibody, Unconjugated (BA1039); Goat Anti-Mouse IgG (H+L) Secondary Antibody, Unconjugated (BA1038)

Atmospheric Particulate Matter Impairs Cognition by Modulating Synaptic Function via the Nose-to-Brain Route

Authors: Liang, C., Jiang, Y., Zhang, T., Ji, Y., Zhang, Y., Sun, Y., Li, S., Qi, Y...
Journal: Science of The Total Environment

Atmospheric particulate matter (PM), a ubiquitous air pollutant, is the leading environmental risk factor for mortality worldwide. Experimental and epidemiological studies consistently suggest a strong link between long-term exposure to PM2.5 (<2.5 μm, fine PM) and cognitive impairment. The neuroinflammatory response is presumed to...

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Cited Boster Product(s): Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (EK0394); Mouse TNF Alpha/Tumor Necrosis Factor ELISA Kit PicoKine® (EK0527); Mouse IL-6/Interleukin-6 ELISA Kit PicoKine® (EK0411)

HOXA5 inhibits the proliferation of extrahepatic cholangiocarcinoma cells by enhancing MXD1 expression and activating the p53 pathway

Authors: Xiong, F., Liu, W., Wang, X., Wu, G., Wang, Q., Guo, T., Huang, W...
Journal: Cell Death & Disease

Homeobox A5 (HOXA5) is a transcription factor in mammalian and can regulate cell differentiation, proliferation, and apoptosis as well as tumorigenesis. However, little is known on whether and how HOXA5 can regulate the malignant behaviors of cholangiocarcinoma. The methylation levels of HOXA5 were evaluated by methylation microarray...

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Cited Boster Product(s): Anti-AIF/AIFM1 Antibody Picoband™ (Monoclonal, 2I5) (M01571-1); HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054); HRP Conjugated AffiniPure Goat Anti-Mouse IgG (H+L) (BA1050)

CDK12 orchestrates super-enhancer-associated CCDC137 transcription to direct hepatic metastasis in colorectal cancer

Authors: Dai, W., Wu, J., Peng, X., Hou, W., Huang, H., Cheng, Q., Liu, Z...
Journal: Clinical and Translational Medicine

Background: Hepatic metastasis is the primary and direct cause of death in individuals with colorectal cancer (CRC) attribute to lack of effective therapeutic targets. The present study aimed to identify potential druggable candidate targets for patients with liver metastatic CRC. Methods: The transcriptional profiles of super-enhancers...

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Cited Boster Product(s): Anti-CDK13 Antibody Picoband™ (A05292-1)

DNA Topoisomerase II-α Regulated by miR-22-5p Promotes Hepatocellular Carcinoma Invasion and Migration through the Hippo Pathway

Authors: Zhao, H., Chen, C., Song, H., Qin, R., Wang, X., He, Q., Li, F…
Journal: Oxidative Medicine and Cellular Longevity

DNA topoisomerases (TOPs) are dysregulated in various types of cancer. However, how TOP II-alpha (TOP2A) contributes to hepatocellular carcinoma (HCC) progression remains elusive. Cohort analysis revealed that the increased expression of TOP2A was associated with poor clinical outcomes and TOP2A was significantly upregulated in HCC...

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Cited Boster Product(s): RIPA Lysis Buffer (AR0105-100); SDS-PAGE Protein Loading Buffer 5X (Reducing) (AR1112); Anti-GAPDH Antibody Picoband™ (A00227-1)